Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract

A standard protocol to develop type 1 diabetes in zebrafish is still uncertain due to unpredictable factors. In this study, an optimized protocol was developed and used to evaluate the anti-diabetic activity of Psychotria malayana leaf. The aims of this study were to develop a type 1 diabetic adu...

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Main Authors:	Benchoula, Khaled, Khatib, Alfi, Quzwain, Fairuz M. C., Che Mohamad, Che Anuar, Wan Sulaiman, Wan Mohd Azizi, Abdul Wahab, Ridhwan, Ahmed, Qamar Uddin, Abdul Ghaffar, Majid, Saiman, Mohd Zuwairi, Alajmi, Mohamed Fahad, El-Seedi, Hesham
Format:	Article
Language:	English English English
Published:	MDPI 2019
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/1/72563%20Optimization%20of%20Hyperglycemic%20Induction.pdf http://irep.iium.edu.my/72563/2/72563%20Optimization%20of%20Hyperglycemic%20Induction%20SCOPUS.pdf http://irep.iium.edu.my/72563/13/72563_Optimization%20of%20Hyperglycemic%20Induction%20in%20Zebrafish%20and%20Evaluation%20of%20Its%20Blood%20Glucose%20Level%20and%20Metabolite%20Fingerprint%20Treated%20with%20Psychotria%20malayana%20Jack%20Leaf%20Extract_wos.pdf

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recordtype	eprints
spelling	iium-725632019-07-31T07:12:13Z http://irep.iium.edu.my/72563/ Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract Benchoula, Khaled Khatib, Alfi Quzwain, Fairuz M. C. Che Mohamad, Che Anuar Wan Sulaiman, Wan Mohd Azizi Abdul Wahab, Ridhwan Ahmed, Qamar Uddin Abdul Ghaffar, Majid Saiman, Mohd Zuwairi Alajmi, Mohamed Fahad El-Seedi, Hesham RS Pharmacy and materia medica A standard protocol to develop type 1 diabetes in zebrafish is still uncertain due to unpredictable factors. In this study, an optimized protocol was developed and used to evaluate the anti-diabetic activity of Psychotria malayana leaf. The aims of this study were to develop a type 1 diabetic adult zebrafish model and to evaluate the anti-diabetic activity of the plant extract on the developed model. The ability of streptozotocin and alloxan at a different dose to elevate the blood glucose levels in zebrafish was evaluated. While the anti-diabetic activity of P. malayana aqueous extract was evaluated through analysis of blood glucose and LC-MS analysis fingerprinting. The results indicated that a single intraperitoneal injection of 300 mg/kg alloxan was the optimal dose to elevate the fasting blood glucose in zebrafish. Furthermore, the plant extract at 1, 2, and 3 g/kg significantly reduced blood glucose levels in the diabetic zebrafish. In addition, LC-MS-based fingerprinting indicated that 3 g/kg plant extract more effective than other doses. Phytosterols, sugar alcohols, sugar acid, free fatty acids, cyclitols, phenolics, and alkaloid were detected in the extract using GC-MS. In conclusion, P. malayana leaf aqueous extract showed anti-diabetic activity on the developed type 1 diabetic zebrafish model. MDPI 2019-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/72563/1/72563%20Optimization%20of%20Hyperglycemic%20Induction.pdf application/pdf en http://irep.iium.edu.my/72563/2/72563%20Optimization%20of%20Hyperglycemic%20Induction%20SCOPUS.pdf application/pdf en http://irep.iium.edu.my/72563/13/72563_Optimization%20of%20Hyperglycemic%20Induction%20in%20Zebrafish%20and%20Evaluation%20of%20Its%20Blood%20Glucose%20Level%20and%20Metabolite%20Fingerprint%20Treated%20with%20Psychotria%20malayana%20Jack%20Leaf%20Extract_wos.pdf Benchoula, Khaled and Khatib, Alfi and Quzwain, Fairuz M. C. and Che Mohamad, Che Anuar and Wan Sulaiman, Wan Mohd Azizi and Abdul Wahab, Ridhwan and Ahmed, Qamar Uddin and Abdul Ghaffar, Majid and Saiman, Mohd Zuwairi and Alajmi, Mohamed Fahad and El-Seedi, Hesham (2019) Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract. Molecules, 24 (8). pp. 1-22. ISSN 1420-3049 https://www.mdpi.com/1420-3049/24/8/1506 /10.3390/molecules24081506
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Benchoula, Khaled Khatib, Alfi Quzwain, Fairuz M. C. Che Mohamad, Che Anuar Wan Sulaiman, Wan Mohd Azizi Abdul Wahab, Ridhwan Ahmed, Qamar Uddin Abdul Ghaffar, Majid Saiman, Mohd Zuwairi Alajmi, Mohamed Fahad El-Seedi, Hesham Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract
description	A standard protocol to develop type 1 diabetes in zebrafish is still uncertain due to unpredictable factors. In this study, an optimized protocol was developed and used to evaluate the anti-diabetic activity of Psychotria malayana leaf. The aims of this study were to develop a type 1 diabetic adult zebrafish model and to evaluate the anti-diabetic activity of the plant extract on the developed model. The ability of streptozotocin and alloxan at a different dose to elevate the blood glucose levels in zebrafish was evaluated. While the anti-diabetic activity of P. malayana aqueous extract was evaluated through analysis of blood glucose and LC-MS analysis fingerprinting. The results indicated that a single intraperitoneal injection of 300 mg/kg alloxan was the optimal dose to elevate the fasting blood glucose in zebrafish. Furthermore, the plant extract at 1, 2, and 3 g/kg significantly reduced blood glucose levels in the diabetic zebrafish. In addition, LC-MS-based fingerprinting indicated that 3 g/kg plant extract more effective than other doses. Phytosterols, sugar alcohols, sugar acid, free fatty acids, cyclitols, phenolics, and alkaloid were detected in the extract using GC-MS. In conclusion, P. malayana leaf aqueous extract showed anti-diabetic activity on the developed type 1 diabetic zebrafish model.
format	Article
author	Benchoula, Khaled Khatib, Alfi Quzwain, Fairuz M. C. Che Mohamad, Che Anuar Wan Sulaiman, Wan Mohd Azizi Abdul Wahab, Ridhwan Ahmed, Qamar Uddin Abdul Ghaffar, Majid Saiman, Mohd Zuwairi Alajmi, Mohamed Fahad El-Seedi, Hesham
author_facet	Benchoula, Khaled Khatib, Alfi Quzwain, Fairuz M. C. Che Mohamad, Che Anuar Wan Sulaiman, Wan Mohd Azizi Abdul Wahab, Ridhwan Ahmed, Qamar Uddin Abdul Ghaffar, Majid Saiman, Mohd Zuwairi Alajmi, Mohamed Fahad El-Seedi, Hesham
author_sort	Benchoula, Khaled
title	Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract
title_short	Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract
title_full	Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract
title_fullStr	Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract
title_full_unstemmed	Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract
title_sort	optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana jack leaf extract
publisher	MDPI
publishDate	2019
url	http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/1/72563%20Optimization%20of%20Hyperglycemic%20Induction.pdf http://irep.iium.edu.my/72563/2/72563%20Optimization%20of%20Hyperglycemic%20Induction%20SCOPUS.pdf http://irep.iium.edu.my/72563/13/72563_Optimization%20of%20Hyperglycemic%20Induction%20in%20Zebrafish%20and%20Evaluation%20of%20Its%20Blood%20Glucose%20Level%20and%20Metabolite%20Fingerprint%20Treated%20with%20Psychotria%20malayana%20Jack%20Leaf%20Extract_wos.pdf
first_indexed	2023-09-18T21:42:48Z
last_indexed	2023-09-18T21:42:48Z
_version_	1777413252920115200

Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract

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