Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract
A standard protocol to develop type 1 diabetes in zebrafish is still uncertain due to unpredictable factors. In this study, an optimized protocol was developed and used to evaluate the anti-diabetic activity of Psychotria malayana leaf. The aims of this study were to develop a type 1 diabetic adu...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
MDPI
2019
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/ http://irep.iium.edu.my/72563/1/72563%20Optimization%20of%20Hyperglycemic%20Induction.pdf http://irep.iium.edu.my/72563/2/72563%20Optimization%20of%20Hyperglycemic%20Induction%20SCOPUS.pdf http://irep.iium.edu.my/72563/13/72563_Optimization%20of%20Hyperglycemic%20Induction%20in%20Zebrafish%20and%20Evaluation%20of%20Its%20Blood%20Glucose%20Level%20and%20Metabolite%20Fingerprint%20Treated%20with%20Psychotria%20malayana%20Jack%20Leaf%20Extract_wos.pdf |
| Summary: | A standard protocol to develop type 1 diabetes in zebrafish is still uncertain due to
unpredictable factors. In this study, an optimized protocol was developed and used to evaluate the
anti-diabetic activity of Psychotria malayana leaf. The aims of this study were to develop a type 1 diabetic
adult zebrafish model and to evaluate the anti-diabetic activity of the plant extract on the developed
model. The ability of streptozotocin and alloxan at a different dose to elevate the blood glucose
levels in zebrafish was evaluated. While the anti-diabetic activity of P. malayana aqueous extract
was evaluated through analysis of blood glucose and LC-MS analysis fingerprinting. The results
indicated that a single intraperitoneal injection of 300 mg/kg alloxan was the optimal dose to elevate
the fasting blood glucose in zebrafish. Furthermore, the plant extract at 1, 2, and 3 g/kg significantly
reduced blood glucose levels in the diabetic zebrafish. In addition, LC-MS-based fingerprinting
indicated that 3 g/kg plant extract more effective than other doses. Phytosterols, sugar alcohols,
sugar acid, free fatty acids, cyclitols, phenolics, and alkaloid were detected in the extract using GC-MS.
In conclusion, P. malayana leaf aqueous extract showed anti-diabetic activity on the developed type
1 diabetic zebrafish model. |
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