Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design

Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from t...

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Main Authors:	Jamal, Parveen, Azmi, Saiful Mohammad Nizam, Amid, Azura
Format:	Conference or Workshop Item
Language:	English
Published:	2013
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://irep.iium.edu.my/33526/ http://irep.iium.edu.my/33526/1/ICBIOE_2.pdf

id	iium-33526
recordtype	eprints
spelling	iium-335262013-12-19T04:41:39Z http://irep.iium.edu.my/33526/ Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura TA Engineering (General). Civil engineering (General) Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from the leaves of Morinda elliptica was performed by using 70% methanol. Optimization of process parameters such as extraction temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of sample to solvent (1g/ml) at five levels was carried out using central composite design (CCD) for the improvement of activity to treat gout. The analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity was improved at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized conditions were verified and the percentage of XO inhibitory activity obtained was 88.93%. The results are encouraging to formulate food, nutraceutical or pharmaceutical products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to irresponsive synthetic XOI. 2013-07-02 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/33526/1/ICBIOE_2.pdf Jamal, Parveen and Azmi, Saiful Mohammad Nizam and Amid, Azura (2013) Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design. In: International Conference on Biotechnology Engineering (ICBioE 2013), 2 – 4 Jul 2013, Kuala Lumpur, Malaysia.
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
description	Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from the leaves of Morinda elliptica was performed by using 70% methanol. Optimization of process parameters such as extraction temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of sample to solvent (1g/ml) at five levels was carried out using central composite design (CCD) for the improvement of activity to treat gout. The analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity was improved at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized conditions were verified and the percentage of XO inhibitory activity obtained was 88.93%. The results are encouraging to formulate food, nutraceutical or pharmaceutical products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to irresponsive synthetic XOI.
format	Conference or Workshop Item
author	Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura
author_facet	Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura
author_sort	Jamal, Parveen
title	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_short	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_full	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_fullStr	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_full_unstemmed	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_sort	development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
publishDate	2013
url	http://irep.iium.edu.my/33526/ http://irep.iium.edu.my/33526/1/ICBIOE_2.pdf
first_indexed	2023-09-18T20:48:28Z
last_indexed	2023-09-18T20:48:28Z
_version_	1777409833816817664

Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design

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