Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria
This study concerned with the evaluation of antibacterial activity of four crude extracts of three parts (leaf, root and bark) of Neem (Azadirachta Indica) obtained by four solvents (chloroform, acetone, cold and hot ethanol). The antibacterial activity of these extracts was assessed against two mul...
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| Format: | Undergraduates Project Papers |
| Language: | English |
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2012
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| Online Access: | http://umpir.ump.edu.my/id/eprint/7815/ http://umpir.ump.edu.my/id/eprint/7815/ http://umpir.ump.edu.my/id/eprint/7815/1/NG_YIN_BOON.PDF |
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ump-7815 |
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eprints |
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ump-78152015-03-03T09:38:15Z http://umpir.ump.edu.my/id/eprint/7815/ Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria Ng , Yin Boon QR Microbiology This study concerned with the evaluation of antibacterial activity of four crude extracts of three parts (leaf, root and bark) of Neem (Azadirachta Indica) obtained by four solvents (chloroform, acetone, cold and hot ethanol). The antibacterial activity of these extracts was assessed against two multiple-drug-resistance bacteria strains, namely E. coli and S. aureus by the conventional well diffusion method. For the antibacterial bioassay, four concentrations (0. 1, 0.2, 0.4 and 0.8%) of each solvent and -part extract solutions were prepared; DMSO was used to solubilize the extract in water. 1% of DMSO was used as control. All extracts were active against both test organisms E. coli and S .aureus, the root acetone displayed overall more potent activity and reaches 52.5% and 51.88% respectively with concentration of 0.8% and the lowest activity observed with bark hot ethanol at concentration of 0.8%. Bioactive groups such as alkaloids and sesquiterpene lactones were screened by thin layer chromatography; and the results obtained were negative for alkaloids and positive for sesquiterpene lactones. The experimental results obtained from this study suggest that Neem (Azadirachta Indica) extracts are promising as natural antibacterial and this may warrant further research to determine the bioactive compound (s). 2012 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7815/1/NG_YIN_BOON.PDF Ng , Yin Boon (2012) Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria. Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:76334&theme=UMP2 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
Universiti Malaysia Pahang |
| building |
UMP Institutional Repository |
| collection |
Online Access |
| language |
English |
| topic |
QR Microbiology |
| spellingShingle |
QR Microbiology Ng , Yin Boon Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria |
| description |
This study concerned with the evaluation of antibacterial activity of four crude extracts of three parts (leaf, root and bark) of Neem (Azadirachta Indica) obtained by four solvents (chloroform, acetone, cold and hot ethanol). The antibacterial activity of these extracts was assessed against two multiple-drug-resistance bacteria strains, namely E. coli and S. aureus by the conventional well diffusion method. For the antibacterial bioassay, four concentrations (0. 1, 0.2, 0.4 and 0.8%) of each solvent and -part extract solutions were prepared; DMSO was used to solubilize the extract in water. 1% of DMSO was used as control. All extracts were active against both test organisms
E. coli and S .aureus, the root acetone displayed overall more potent activity and reaches 52.5% and 51.88% respectively with concentration of 0.8% and the lowest activity observed with bark hot ethanol at concentration of 0.8%. Bioactive groups such as alkaloids and sesquiterpene lactones were screened by thin layer chromatography; and the results obtained were negative for alkaloids and positive for sesquiterpene lactones. The experimental results obtained from this study suggest that Neem (Azadirachta Indica) extracts are promising as natural antibacterial and this may warrant further research to determine the bioactive compound (s). |
| format |
Undergraduates Project Papers |
| author |
Ng , Yin Boon |
| author_facet |
Ng , Yin Boon |
| author_sort |
Ng , Yin Boon |
| title |
Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria |
| title_short |
Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria |
| title_full |
Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria |
| title_fullStr |
Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria |
| title_full_unstemmed |
Antibacterial activity of neem (Azadirachta Indica) extracts against multiple drug resistant (MDR) bacteria |
| title_sort |
antibacterial activity of neem (azadirachta indica) extracts against multiple drug resistant (mdr) bacteria |
| publishDate |
2012 |
| url |
http://umpir.ump.edu.my/id/eprint/7815/ http://umpir.ump.edu.my/id/eprint/7815/ http://umpir.ump.edu.my/id/eprint/7815/1/NG_YIN_BOON.PDF |
| first_indexed |
2023-09-18T22:04:50Z |
| last_indexed |
2023-09-18T22:04:50Z |
| _version_ |
1777414638566113280 |