Effects of media components on arachidonic acid production by Candida krusei under submerged condition

Arachidonic acid (ARA) is a long chain polyunsaturated fatty acid (PuFA) of the omega-6 class (5,8,11,14-eico-satetraenoic acid). It plays an important role in the structure and function of biological membranes (Yu et al., 2002), which also as an essential fatty acid in human nutrition and a biogene...

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Main Author:	Laing, Penelope Ering
Format:	Undergraduates Project Papers
Language:	English
Published:	2013
Subjects:	QP Physiology
Online Access:	http://umpir.ump.edu.my/id/eprint/7237/ http://umpir.ump.edu.my/id/eprint/7237/ http://umpir.ump.edu.my/id/eprint/7237/1/Effects_of_media_components_on_arachidonic_acid_production.pdf

id	ump-7237
recordtype	eprints
spelling	ump-72372015-03-03T09:34:55Z http://umpir.ump.edu.my/id/eprint/7237/ Effects of media components on arachidonic acid production by Candida krusei under submerged condition Laing, Penelope Ering QP Physiology Arachidonic acid (ARA) is a long chain polyunsaturated fatty acid (PuFA) of the omega-6 class (5,8,11,14-eico-satetraenoic acid). It plays an important role in the structure and function of biological membranes (Yu et al., 2002), which also as an essential fatty acid in human nutrition and a biogenetic precursor. The aim of this study is to develop an optimum media component (glucose, potassium nitrate (KNO3), potassium phosphate monobasic (KH2PO4), and glutamate) to yield the highest production of ARA by Candida krusei under submerged condition. The media components are studied at different levels which are from 10 to 80 g/L for glucose, 1 to 5 g/L for KNO3, 1 to 5 g/L for KH2PO4 and 0 to 0.8 g/L for glutamatewhich are determined using Design Expert. The total ARA composition will be determined by gas chromatography (GC) with capillary column 0.25 mm x 30 m x 0.25 µm, using helium as the carrier gas and at initial column temperature at 185 °C which then will increase at a rate of 3.5 °C min-1 to 235 °C.From this study, the expected optimum conditions that will be achieved is with glucose concentration, 10.0 g/L, KH2PO4 concentration, 1.0 g/L, KNO3 concentration 1.0 g/L and concentration of glutamic acid, 0.8 g/L which resulted in maximum production of ARA(1.39 g/L). In a conclusion, the optimization of media to produce high amount of ARA using microorganism is a suitable alternative way to produce ARA which benefits everyone besides getting ARA from porcine liver which is prohibited in certain community such as the Muslims and vegetarians. 2013 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/7237/1/Effects_of_media_components_on_arachidonic_acid_production.pdf Laing, Penelope Ering (2013) Effects of media components on arachidonic acid production by Candida krusei under submerged condition. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:76108&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QP Physiology
spellingShingle	QP Physiology Laing, Penelope Ering Effects of media components on arachidonic acid production by Candida krusei under submerged condition
description	Arachidonic acid (ARA) is a long chain polyunsaturated fatty acid (PuFA) of the omega-6 class (5,8,11,14-eico-satetraenoic acid). It plays an important role in the structure and function of biological membranes (Yu et al., 2002), which also as an essential fatty acid in human nutrition and a biogenetic precursor. The aim of this study is to develop an optimum media component (glucose, potassium nitrate (KNO3), potassium phosphate monobasic (KH2PO4), and glutamate) to yield the highest production of ARA by Candida krusei under submerged condition. The media components are studied at different levels which are from 10 to 80 g/L for glucose, 1 to 5 g/L for KNO3, 1 to 5 g/L for KH2PO4 and 0 to 0.8 g/L for glutamatewhich are determined using Design Expert. The total ARA composition will be determined by gas chromatography (GC) with capillary column 0.25 mm x 30 m x 0.25 µm, using helium as the carrier gas and at initial column temperature at 185 °C which then will increase at a rate of 3.5 °C min-1 to 235 °C.From this study, the expected optimum conditions that will be achieved is with glucose concentration, 10.0 g/L, KH2PO4 concentration, 1.0 g/L, KNO3 concentration 1.0 g/L and concentration of glutamic acid, 0.8 g/L which resulted in maximum production of ARA(1.39 g/L). In a conclusion, the optimization of media to produce high amount of ARA using microorganism is a suitable alternative way to produce ARA which benefits everyone besides getting ARA from porcine liver which is prohibited in certain community such as the Muslims and vegetarians.
format	Undergraduates Project Papers
author	Laing, Penelope Ering
author_facet	Laing, Penelope Ering
author_sort	Laing, Penelope Ering
title	Effects of media components on arachidonic acid production by Candida krusei under submerged condition
title_short	Effects of media components on arachidonic acid production by Candida krusei under submerged condition
title_full	Effects of media components on arachidonic acid production by Candida krusei under submerged condition
title_fullStr	Effects of media components on arachidonic acid production by Candida krusei under submerged condition
title_full_unstemmed	Effects of media components on arachidonic acid production by Candida krusei under submerged condition
title_sort	effects of media components on arachidonic acid production by candida krusei under submerged condition
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/7237/ http://umpir.ump.edu.my/id/eprint/7237/ http://umpir.ump.edu.my/id/eprint/7237/1/Effects_of_media_components_on_arachidonic_acid_production.pdf
first_indexed	2023-09-18T22:03:46Z
last_indexed	2023-09-18T22:03:46Z
_version_	1777414571779162112

Effects of media components on arachidonic acid production by Candida krusei under submerged condition

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