Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation

The mixed matrix membrane (MMM) concept is extended, for the first time, to produce a hydrophobic interaction chromatography (HIC) membrane using crushed Phenyl Sepharose™ (GE Healthcare Technologies, Uppsala, Sweden) resin and tested for use in whey protein fractionation. The HIC MMM had static bin...

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Main Authors: Syed Mohd Saufi, Tuan Chik, Fee, Conan J.
Format: Article
Published: Elsevier Ltd 2013
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/6215/
http://umpir.ump.edu.my/id/eprint/6215/
http://umpir.ump.edu.my/id/eprint/6215/
id ump-6215
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spelling ump-62152018-04-11T01:57:38Z http://umpir.ump.edu.my/id/eprint/6215/ Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation Syed Mohd Saufi, Tuan Chik Fee, Conan J. TP Chemical technology The mixed matrix membrane (MMM) concept is extended, for the first time, to produce a hydrophobic interaction chromatography (HIC) membrane using crushed Phenyl Sepharose™ (GE Healthcare Technologies, Uppsala, Sweden) resin and tested for use in whey protein fractionation. The HIC MMM had static binding capacities (membrane volume basis) of 18.419±0.605 mg mL−1 for β-lactoglobulin, 45.850±2.753 mg mL−1 for α-lactalbumin, 41.106±9.004 mg mL−1 for bovine serum albumin and 42.467±4.130 mg mL−1 for lactoferin in individual protein solutions. For flowthrough whey fractionation, the adsorption performance of the HIC MMM was similar to a HiTrap™ Phenyl (GE Healthcare Technologies) HIC column. However, the well-known high processing speeds and inherently low pressure drops of MMM chromatography may offer benefits over a conventional packed bed column. Elsevier Ltd 2013 Article PeerReviewed Syed Mohd Saufi, Tuan Chik and Fee, Conan J. (2013) Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation. Journal of Membrane Science, 444. pp. 157-163. ISSN 0376-7388 http://dx.doi.org/10.1016/j.memsci.2013.05.007 DOI: 10.1016/j.memsci.2013.05.007
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
topic TP Chemical technology
spellingShingle TP Chemical technology
Syed Mohd Saufi, Tuan Chik
Fee, Conan J.
Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation
description The mixed matrix membrane (MMM) concept is extended, for the first time, to produce a hydrophobic interaction chromatography (HIC) membrane using crushed Phenyl Sepharose™ (GE Healthcare Technologies, Uppsala, Sweden) resin and tested for use in whey protein fractionation. The HIC MMM had static binding capacities (membrane volume basis) of 18.419±0.605 mg mL−1 for β-lactoglobulin, 45.850±2.753 mg mL−1 for α-lactalbumin, 41.106±9.004 mg mL−1 for bovine serum albumin and 42.467±4.130 mg mL−1 for lactoferin in individual protein solutions. For flowthrough whey fractionation, the adsorption performance of the HIC MMM was similar to a HiTrap™ Phenyl (GE Healthcare Technologies) HIC column. However, the well-known high processing speeds and inherently low pressure drops of MMM chromatography may offer benefits over a conventional packed bed column.
format Article
author Syed Mohd Saufi, Tuan Chik
Fee, Conan J.
author_facet Syed Mohd Saufi, Tuan Chik
Fee, Conan J.
author_sort Syed Mohd Saufi, Tuan Chik
title Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation
title_short Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation
title_full Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation
title_fullStr Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation
title_full_unstemmed Mixed Matrix Membrane Chromatography Based on Hydrophobic Interaction for Whey Protein Fractionation
title_sort mixed matrix membrane chromatography based on hydrophobic interaction for whey protein fractionation
publisher Elsevier Ltd
publishDate 2013
url http://umpir.ump.edu.my/id/eprint/6215/
http://umpir.ump.edu.my/id/eprint/6215/
http://umpir.ump.edu.my/id/eprint/6215/
first_indexed 2023-09-18T22:01:48Z
last_indexed 2023-09-18T22:01:48Z
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