Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy

White tea (WT) presents high levels of catechins, which are known to reduce oxidative stress. WT is the least processed tea, unfermented and prepared only from very young tea leaves. The subject of this paper is the use of the spin trap method and electron paramagnetic resonance (EPR) spectroscopy a...

Full description

Bibliographic Details
Main Authors: Nurul Aini, Mohd Azman, Peiro, Sara, Fajari, Lluis, Julia, Luis, Almajano, María Pilar
Format: Article
Language:English
Published: ACS Publications 2014
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/6082/
http://umpir.ump.edu.my/id/eprint/6082/
http://umpir.ump.edu.my/id/eprint/6082/
http://umpir.ump.edu.my/id/eprint/6082/1/fkksa-2014-nurul-Radical%20Scavenging%20of%20White.pdf
id ump-6082
recordtype eprints
spelling ump-60822016-04-20T03:18:12Z http://umpir.ump.edu.my/id/eprint/6082/ Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy Nurul Aini, Mohd Azman Peiro, Sara Fajari, Lluis Julia, Luis Almajano, María Pilar TP Chemical technology White tea (WT) presents high levels of catechins, which are known to reduce oxidative stress. WT is the least processed tea, unfermented and prepared only from very young tea leaves. The subject of this paper is the use of the spin trap method and electron paramagnetic resonance (EPR) spectroscopy as the analytical tool to measure, for the first time, the radical scavenging activity of WT and its major catechin components, epicatechin (EC), epicatechin-3-gallate (ECG), epigallocatechin (EGC), and epigallocatechin-3-gallate (EGCG), against the methoxy radical, using ferulic acid as antioxidant pattern. The antioxidant activity has been measured by the decrease of the intensity of the spectral bands of the adduct DMPO−OCH in the EPR with the amount of antioxidant in the reactive mixture. Tea leaves and buds were extracted with waterless methanol. It has been proved that tea compounds with more antiradical activity against methoxy radical are those with the gallate group, EGCG and ECG. ACS Publications 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6082/1/fkksa-2014-nurul-Radical%20Scavenging%20of%20White.pdf Nurul Aini, Mohd Azman and Peiro, Sara and Fajari, Lluis and Julia, Luis and Almajano, María Pilar (2014) Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy. Journal of Agricultural and Food Chemistry, 62 (25). pp. 5743-5748. ISSN 0021-8561 (print); 1520-5118 (online) http://dx.doi.org/10.1021/jf501707p DOI: 10.1021/jf501707p
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nurul Aini, Mohd Azman
Peiro, Sara
Fajari, Lluis
Julia, Luis
Almajano, María Pilar
Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy
description White tea (WT) presents high levels of catechins, which are known to reduce oxidative stress. WT is the least processed tea, unfermented and prepared only from very young tea leaves. The subject of this paper is the use of the spin trap method and electron paramagnetic resonance (EPR) spectroscopy as the analytical tool to measure, for the first time, the radical scavenging activity of WT and its major catechin components, epicatechin (EC), epicatechin-3-gallate (ECG), epigallocatechin (EGC), and epigallocatechin-3-gallate (EGCG), against the methoxy radical, using ferulic acid as antioxidant pattern. The antioxidant activity has been measured by the decrease of the intensity of the spectral bands of the adduct DMPO−OCH in the EPR with the amount of antioxidant in the reactive mixture. Tea leaves and buds were extracted with waterless methanol. It has been proved that tea compounds with more antiradical activity against methoxy radical are those with the gallate group, EGCG and ECG.
format Article
author Nurul Aini, Mohd Azman
Peiro, Sara
Fajari, Lluis
Julia, Luis
Almajano, María Pilar
author_facet Nurul Aini, Mohd Azman
Peiro, Sara
Fajari, Lluis
Julia, Luis
Almajano, María Pilar
author_sort Nurul Aini, Mohd Azman
title Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy
title_short Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy
title_full Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy
title_fullStr Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy
title_full_unstemmed Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy
title_sort radical scavenging of white tea and its flavonoid constituents by electron paramagnetic resonance (epr) spectroscopy
publisher ACS Publications
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/6082/
http://umpir.ump.edu.my/id/eprint/6082/
http://umpir.ump.edu.my/id/eprint/6082/
http://umpir.ump.edu.my/id/eprint/6082/1/fkksa-2014-nurul-Radical%20Scavenging%20of%20White.pdf
first_indexed 2023-09-18T22:01:32Z
last_indexed 2023-09-18T22:01:32Z
_version_ 1777414430719475712