Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy
White tea (WT) presents high levels of catechins, which are known to reduce oxidative stress. WT is the least processed tea, unfermented and prepared only from very young tea leaves. The subject of this paper is the use of the spin trap method and electron paramagnetic resonance (EPR) spectroscopy a...
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ump-60822016-04-20T03:18:12Z http://umpir.ump.edu.my/id/eprint/6082/ Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy Nurul Aini, Mohd Azman Peiro, Sara Fajari, Lluis Julia, Luis Almajano, María Pilar TP Chemical technology White tea (WT) presents high levels of catechins, which are known to reduce oxidative stress. WT is the least processed tea, unfermented and prepared only from very young tea leaves. The subject of this paper is the use of the spin trap method and electron paramagnetic resonance (EPR) spectroscopy as the analytical tool to measure, for the first time, the radical scavenging activity of WT and its major catechin components, epicatechin (EC), epicatechin-3-gallate (ECG), epigallocatechin (EGC), and epigallocatechin-3-gallate (EGCG), against the methoxy radical, using ferulic acid as antioxidant pattern. The antioxidant activity has been measured by the decrease of the intensity of the spectral bands of the adduct DMPO−OCH in the EPR with the amount of antioxidant in the reactive mixture. Tea leaves and buds were extracted with waterless methanol. It has been proved that tea compounds with more antiradical activity against methoxy radical are those with the gallate group, EGCG and ECG. ACS Publications 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6082/1/fkksa-2014-nurul-Radical%20Scavenging%20of%20White.pdf Nurul Aini, Mohd Azman and Peiro, Sara and Fajari, Lluis and Julia, Luis and Almajano, María Pilar (2014) Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy. Journal of Agricultural and Food Chemistry, 62 (25). pp. 5743-5748. ISSN 0021-8561 (print); 1520-5118 (online) http://dx.doi.org/10.1021/jf501707p DOI: 10.1021/jf501707p |
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TP Chemical technology Nurul Aini, Mohd Azman Peiro, Sara Fajari, Lluis Julia, Luis Almajano, María Pilar Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy |
description |
White tea (WT) presents high levels of catechins, which are known to reduce oxidative stress. WT is the least processed tea, unfermented and prepared only from very young tea leaves. The subject of this paper is the use of the spin trap method and electron paramagnetic resonance (EPR) spectroscopy as the analytical tool to measure, for the first time, the radical scavenging activity of WT and its major catechin components, epicatechin (EC), epicatechin-3-gallate (ECG), epigallocatechin (EGC), and epigallocatechin-3-gallate (EGCG), against the methoxy radical, using ferulic acid as antioxidant pattern. The antioxidant activity has been measured by the decrease of the intensity of the spectral bands of the adduct DMPO−OCH in the EPR with the amount of antioxidant in the reactive mixture. Tea leaves and buds were extracted with waterless methanol. It has been proved that tea compounds with more antiradical activity against methoxy radical are those with the gallate group, EGCG and ECG. |
format |
Article |
author |
Nurul Aini, Mohd Azman Peiro, Sara Fajari, Lluis Julia, Luis Almajano, María Pilar |
author_facet |
Nurul Aini, Mohd Azman Peiro, Sara Fajari, Lluis Julia, Luis Almajano, María Pilar |
author_sort |
Nurul Aini, Mohd Azman |
title |
Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy |
title_short |
Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy |
title_full |
Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy |
title_fullStr |
Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy |
title_full_unstemmed |
Radical Scavenging of White Tea and Its Flavonoid Constituents by Electron Paramagnetic Resonance (EPR) Spectroscopy |
title_sort |
radical scavenging of white tea and its flavonoid constituents by electron paramagnetic resonance (epr) spectroscopy |
publisher |
ACS Publications |
publishDate |
2014 |
url |
http://umpir.ump.edu.my/id/eprint/6082/ http://umpir.ump.edu.my/id/eprint/6082/ http://umpir.ump.edu.my/id/eprint/6082/ http://umpir.ump.edu.my/id/eprint/6082/1/fkksa-2014-nurul-Radical%20Scavenging%20of%20White.pdf |
first_indexed |
2023-09-18T22:01:32Z |
last_indexed |
2023-09-18T22:01:32Z |
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1777414430719475712 |