Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation

Determination of kinetic parameters of enzymes is important in biotechnology research. It is also one of the most challenging processes in methanol production. The activity of enzyme is determined in term of initial rates at various substrate concentrations. The enzymatic hydrolysis of methanol by p...

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Main Authors:	Norazwina, Zainol, Siti Natrah, Ismail
Format:	Article
Language:	English
Published:	Department of Chemical Engineering, Diponegoro University 2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/25057/ http://umpir.ump.edu.my/id/eprint/25057/ http://umpir.ump.edu.my/id/eprint/25057/ http://umpir.ump.edu.my/id/eprint/25057/1/Evaluation%20of%20enzyme%20kinetic%20parameters%20to%20produce%20methanol.pdf

id	ump-25057
recordtype	eprints
spelling	ump-250572019-10-24T06:20:46Z http://umpir.ump.edu.my/id/eprint/25057/ Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation Norazwina, Zainol Siti Natrah, Ismail TP Chemical technology Determination of kinetic parameters of enzymes is important in biotechnology research. It is also one of the most challenging processes in methanol production. The activity of enzyme is determined in term of initial rates at various substrate concentrations. The enzymatic hydrolysis of methanol by pectin methyl esterase (PME) enzyme was investigated at 25 °C and pH 9 over the reaction time range from 0 to 90 min. In this study, the parameters of the enzyme's kinetic, KM and Vmax were directly determined using a modified Michaelis-Menten equation by applying the Lineweaver-Burk plots. Besides, nonlinear regression of Michaelis-Menten equation was calculated based on Euler’s and Runge-Kutta 4th order methods by using Solver supplement application. The result of kinetic constant was tested by comparing the experimental data with model predictions. It was found that Euler and Runge-Kutta method was successful in determining the kinetic parameter rather than Lineweaver-Burk plot. The application of the Michaelis-Menten equation describes the enzyme kinetic very well. From the kinetic analysis, it showed the good agreement between the result obtained and the predictions model in the production of methanol using PME enzyme. Department of Chemical Engineering, Diponegoro University 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25057/1/Evaluation%20of%20enzyme%20kinetic%20parameters%20to%20produce%20methanol.pdf Norazwina, Zainol and Siti Natrah, Ismail (2019) Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation. Bulletin of Chemical Reaction Engineering & Catalysis, 14 (2). pp. 436-442. ISSN 1978-2993 https://doi.org/10.9767/bcrec.14.2.3317.436-442 https://doi.org/10.9767/bcrec.14.2.3317.436-442
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Norazwina, Zainol Siti Natrah, Ismail Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
description	Determination of kinetic parameters of enzymes is important in biotechnology research. It is also one of the most challenging processes in methanol production. The activity of enzyme is determined in term of initial rates at various substrate concentrations. The enzymatic hydrolysis of methanol by pectin methyl esterase (PME) enzyme was investigated at 25 °C and pH 9 over the reaction time range from 0 to 90 min. In this study, the parameters of the enzyme's kinetic, KM and Vmax were directly determined using a modified Michaelis-Menten equation by applying the Lineweaver-Burk plots. Besides, nonlinear regression of Michaelis-Menten equation was calculated based on Euler’s and Runge-Kutta 4th order methods by using Solver supplement application. The result of kinetic constant was tested by comparing the experimental data with model predictions. It was found that Euler and Runge-Kutta method was successful in determining the kinetic parameter rather than Lineweaver-Burk plot. The application of the Michaelis-Menten equation describes the enzyme kinetic very well. From the kinetic analysis, it showed the good agreement between the result obtained and the predictions model in the production of methanol using PME enzyme.
format	Article
author	Norazwina, Zainol Siti Natrah, Ismail
author_facet	Norazwina, Zainol Siti Natrah, Ismail
author_sort	Norazwina, Zainol
title	Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_short	Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_full	Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_fullStr	Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_full_unstemmed	Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_sort	evaluation of enzyme kinetic parameters to produce methanol using michaelis-menten equation
publisher	Department of Chemical Engineering, Diponegoro University
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/25057/ http://umpir.ump.edu.my/id/eprint/25057/ http://umpir.ump.edu.my/id/eprint/25057/ http://umpir.ump.edu.my/id/eprint/25057/1/Evaluation%20of%20enzyme%20kinetic%20parameters%20to%20produce%20methanol.pdf
first_indexed	2023-09-18T22:38:17Z
last_indexed	2023-09-18T22:38:17Z
_version_	1777416742714212352

Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation

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