Waste Management Biotechnology for Alkaline Protease Production and Optimization

Environmental pollution is one of the major issues in either well developed or in developing countries. Pollution can also come from animal wastes such as livestock, wastewater and feed that have been wasted. This type of circumstances occurs when there are abundant of waste generated in animal farm...

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Main Authors: Thanoon, Raid D., Rubaaini, Subramaniam, Mahmood, Mahmood H., Munem, Osama A., Makky, Essam A., M. M., Yusoff
Format: Conference or Workshop Item
Language:English
Published: Universiti Malaysia Pahang 2017
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/17619/
http://umpir.ump.edu.my/id/eprint/17619/
http://umpir.ump.edu.my/id/eprint/17619/1/16.%20Waste%20management%20biotechnology%20for%20alkaline%20protease%20production%20and%20optimization.pdf
id ump-17619
recordtype eprints
spelling ump-176192020-02-28T01:27:01Z http://umpir.ump.edu.my/id/eprint/17619/ Waste Management Biotechnology for Alkaline Protease Production and Optimization Thanoon, Raid D. Rubaaini, Subramaniam Mahmood, Mahmood H. Munem, Osama A. Makky, Essam A. M. M., Yusoff TD Environmental technology. Sanitary engineering Environmental pollution is one of the major issues in either well developed or in developing countries. Pollution can also come from animal wastes such as livestock, wastewater and feed that have been wasted. This type of circumstances occurs when there are abundant of waste generated in animal farms in certain area that is difficult to be disposed. For example, the fish residues such as fish fin, viscera, and scales can be one of the sources of contamination that can be the major problem. Moreover, chicken feather (CF) is widely disposed that results in environmental pollution as it cannot be solved with ease. Hence both fish fin (FF) and CF can be utilized to produce alkaline protease (AP) enzyme by using microbial fermentation power. Protein from each sample was extracted and determined. Then, the extracted protein from both wastes was employed in the production media together with bacterial suspension which was incubated. Next, alkaline protease assay was carried out to test for presence of alkaline protease in the samples. Optimization parameters controlling alkaline protease productivity were screened and determined. The optimum pH of CF and FF was 9.0, the optimum temperatures recorded at 28 and 40°C for CF and FF respectively, the production incubation periods were 6 and 10 days for CF and FF respectively. While, galactose and glucose exhibited the optimum carbon sources for CF and FF respectively from one hand, and the optimum nitrogen sources recorded to be ammonium chloride and beef extract for CF and FF respectively from the other hand. Universiti Malaysia Pahang 2017 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17619/1/16.%20Waste%20management%20biotechnology%20for%20alkaline%20protease%20production%20and%20optimization.pdf Thanoon, Raid D. and Rubaaini, Subramaniam and Mahmood, Mahmood H. and Munem, Osama A. and Makky, Essam A. and M. M., Yusoff (2017) Waste Management Biotechnology for Alkaline Protease Production and Optimization. In: Proceedings of the FGIC 1st Conference on Governance & Integrity, 3-4 April 2017 , Yayasan Pahang, Kuantan, Malaysia. pp. 308-322.. ISBN 978-967-2054-37-5 http://fgic.ump.edu.my/images/docman/1st-FGIC-Proceedings.pdf
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TD Environmental technology. Sanitary engineering
spellingShingle TD Environmental technology. Sanitary engineering
Thanoon, Raid D.
Rubaaini, Subramaniam
Mahmood, Mahmood H.
Munem, Osama A.
Makky, Essam A.
M. M., Yusoff
Waste Management Biotechnology for Alkaline Protease Production and Optimization
description Environmental pollution is one of the major issues in either well developed or in developing countries. Pollution can also come from animal wastes such as livestock, wastewater and feed that have been wasted. This type of circumstances occurs when there are abundant of waste generated in animal farms in certain area that is difficult to be disposed. For example, the fish residues such as fish fin, viscera, and scales can be one of the sources of contamination that can be the major problem. Moreover, chicken feather (CF) is widely disposed that results in environmental pollution as it cannot be solved with ease. Hence both fish fin (FF) and CF can be utilized to produce alkaline protease (AP) enzyme by using microbial fermentation power. Protein from each sample was extracted and determined. Then, the extracted protein from both wastes was employed in the production media together with bacterial suspension which was incubated. Next, alkaline protease assay was carried out to test for presence of alkaline protease in the samples. Optimization parameters controlling alkaline protease productivity were screened and determined. The optimum pH of CF and FF was 9.0, the optimum temperatures recorded at 28 and 40°C for CF and FF respectively, the production incubation periods were 6 and 10 days for CF and FF respectively. While, galactose and glucose exhibited the optimum carbon sources for CF and FF respectively from one hand, and the optimum nitrogen sources recorded to be ammonium chloride and beef extract for CF and FF respectively from the other hand.
format Conference or Workshop Item
author Thanoon, Raid D.
Rubaaini, Subramaniam
Mahmood, Mahmood H.
Munem, Osama A.
Makky, Essam A.
M. M., Yusoff
author_facet Thanoon, Raid D.
Rubaaini, Subramaniam
Mahmood, Mahmood H.
Munem, Osama A.
Makky, Essam A.
M. M., Yusoff
author_sort Thanoon, Raid D.
title Waste Management Biotechnology for Alkaline Protease Production and Optimization
title_short Waste Management Biotechnology for Alkaline Protease Production and Optimization
title_full Waste Management Biotechnology for Alkaline Protease Production and Optimization
title_fullStr Waste Management Biotechnology for Alkaline Protease Production and Optimization
title_full_unstemmed Waste Management Biotechnology for Alkaline Protease Production and Optimization
title_sort waste management biotechnology for alkaline protease production and optimization
publisher Universiti Malaysia Pahang
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/17619/
http://umpir.ump.edu.my/id/eprint/17619/
http://umpir.ump.edu.my/id/eprint/17619/1/16.%20Waste%20management%20biotechnology%20for%20alkaline%20protease%20production%20and%20optimization.pdf
first_indexed 2023-09-18T22:24:27Z
last_indexed 2023-09-18T22:24:27Z
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