Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth

Background: A two level (23) factorial design of experiment (DOE) was employed to investigate the influence of nutrients concentrations and main operational parameters on the growth of Bacillus cereus (ATCC 14579) in a shake flask. The factorial models have been established from experimental design...

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Main Authors:	Ahmad, Mani Malam, Abdul Aziz, Mohd Azoddein, Mior Ahmad Khushairi, Mohd Zahari, Mazrul Nizam, Abu Seman
Format:	Article
Language:	English
Published:	AENSI Publishing 2016
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/17304/ http://umpir.ump.edu.my/id/eprint/17304/ http://umpir.ump.edu.my/id/eprint/17304/1/fkksa-2016-mior-Application%20Of%20Factorial%20Design%20To%20The%20Stress.pdf

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spelling	ump-173042018-01-22T03:19:22Z http://umpir.ump.edu.my/id/eprint/17304/ Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth Ahmad, Mani Malam Abdul Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman TP Chemical technology Background: A two level (23) factorial design of experiment (DOE) was employed to investigate the influence of nutrients concentrations and main operational parameters on the growth of Bacillus cereus (ATCC 14579) in a shake flask. The factorial models have been established from experimental design to study the individual and interactions effects toward the response within the selected variables nutrient concentration (4-16gl-1), temperature (300C – 420C), agitation (140rpm-200rpm) and acclimatization time (24hours-72hours). These were statistically validated using analysis of variance (ANOVA). Objective: The present study aimed to use fractional factorial design of experiment to investigate the influence of growth limiting factors to the bacterial growth in a fermenting medium of orbital shaker. Results: The results revealed that the model terms were all significant with F-value of 251.07 at (p <0.004). The model term having the most significant effect on the response was nutrient concentration. And the magnitude of the main influence is in the ascending order nutrient concentration > temperature > acclimatization time. The analysis of the experimental response indicated that the interaction of nutrient concentration and temperature had the highest influence on the response. Whereas the interaction effects of nutrient and acclimatization time was found to be statistically insignificant. Based on the R2 and adjusted R2 the estimated model terms spell high degree of relationship between observed and predicted values, thus the prediction ability of the models is maintained. Conclusion: Although the interaction models terms have significant effects, their levels were only less likely comparable to linear effects. It could therefore concluded that nutrient concentration, temperature and to some extend acclimatization time were four to greatly limit growth at a specific ranges. In general, the predicted value was in reasonable agreement with the experimental data, further confirming the very good prediction ability of the model. AENSI Publishing 2016 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/17304/1/fkksa-2016-mior-Application%20Of%20Factorial%20Design%20To%20The%20Stress.pdf Ahmad, Mani Malam and Abdul Aziz, Mohd Azoddein and Mior Ahmad Khushairi, Mohd Zahari and Mazrul Nizam, Abu Seman (2016) Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth. Australian Journal of Basic and Applied Sciences, 10 (17 Sp.). pp. 148-156. ISSN 1991-8178 http://www.ajbasweb.com/old/ajbas/2016/Special%20ICCEIB/148-156.pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ahmad, Mani Malam Abdul Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth
description	Background: A two level (23) factorial design of experiment (DOE) was employed to investigate the influence of nutrients concentrations and main operational parameters on the growth of Bacillus cereus (ATCC 14579) in a shake flask. The factorial models have been established from experimental design to study the individual and interactions effects toward the response within the selected variables nutrient concentration (4-16gl-1), temperature (300C – 420C), agitation (140rpm-200rpm) and acclimatization time (24hours-72hours). These were statistically validated using analysis of variance (ANOVA). Objective: The present study aimed to use fractional factorial design of experiment to investigate the influence of growth limiting factors to the bacterial growth in a fermenting medium of orbital shaker. Results: The results revealed that the model terms were all significant with F-value of 251.07 at (p <0.004). The model term having the most significant effect on the response was nutrient concentration. And the magnitude of the main influence is in the ascending order nutrient concentration > temperature > acclimatization time. The analysis of the experimental response indicated that the interaction of nutrient concentration and temperature had the highest influence on the response. Whereas the interaction effects of nutrient and acclimatization time was found to be statistically insignificant. Based on the R2 and adjusted R2 the estimated model terms spell high degree of relationship between observed and predicted values, thus the prediction ability of the models is maintained. Conclusion: Although the interaction models terms have significant effects, their levels were only less likely comparable to linear effects. It could therefore concluded that nutrient concentration, temperature and to some extend acclimatization time were four to greatly limit growth at a specific ranges. In general, the predicted value was in reasonable agreement with the experimental data, further confirming the very good prediction ability of the model.
format	Article
author	Ahmad, Mani Malam Abdul Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman
author_facet	Ahmad, Mani Malam Abdul Aziz, Mohd Azoddein Mior Ahmad Khushairi, Mohd Zahari Mazrul Nizam, Abu Seman
author_sort	Ahmad, Mani Malam
title	Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth
title_short	Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth
title_full	Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth
title_fullStr	Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth
title_full_unstemmed	Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth
title_sort	application of factorial design to the stress phenomenon of bacillus cereus (atcc 14579) growth
publisher	AENSI Publishing
publishDate	2016
url	http://umpir.ump.edu.my/id/eprint/17304/ http://umpir.ump.edu.my/id/eprint/17304/ http://umpir.ump.edu.my/id/eprint/17304/1/fkksa-2016-mior-Application%20Of%20Factorial%20Design%20To%20The%20Stress.pdf
first_indexed	2023-09-18T22:23:48Z
last_indexed	2023-09-18T22:23:48Z
_version_	1777415831466016768

Application Of Factorial Design To The Stress Phenomenon Of Bacillus Cereus (Atcc 14579) Growth

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