Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin

In order to optimize the extraction of gelatin from pangasius catfish skin, a response surface method (RSM) involving a Central Composite Design (CCD) was applied. Four variables, namely NaOH concentration (0-0.3 N), acetic acid concentration (0.025-0.125 N), extraction time (2-4 h) and extraction t...

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Main Authors:	Mahmoodani, Fatemeh, Ardekani, Venus Sanaei, See, Siau Fern, Salma Mohamad Yusop, Abdul Salam Babji
Format:	Article
Language:	English
Published:	Universiti Kebangsaan Malaysia 2014
Online Access:	http://journalarticle.ukm.my/7254/ http://journalarticle.ukm.my/7254/ http://journalarticle.ukm.my/7254/1/05_Fatemeh.pdf

id	ukm-7254
recordtype	eprints
spelling	ukm-72542016-12-14T06:43:32Z http://journalarticle.ukm.my/7254/ Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin Mahmoodani, Fatemeh Ardekani, Venus Sanaei See, Siau Fern Salma Mohamad Yusop, Abdul Salam Babji, In order to optimize the extraction of gelatin from pangasius catfish skin, a response surface method (RSM) involving a Central Composite Design (CCD) was applied. Four variables, namely NaOH concentration (0-0.3 N), acetic acid concentration (0.025-0.125 N), extraction time (2-4 h) and extraction temperature (40-80°C) were selected as independent variables for the optimization using RSM. The dependent variable was calculated by hydroxyproline recovery. The optimum conditions for extraction were produced by a pre-treatment of 0.2 N NaOH and 0.1 N acetic acid along with hot water extraction at 63.7°C for 2.41 h. The results showed that the predicted response by RSM (68.53%) closely matched the experimental response of 68.16%. The results indicated that the extracted gelatin possessed high gel strength (438 g) and high content of imino acid (proline and hydroxyproline) (18.01%) with a viscosity of 4.67 mPa s. The results showed that RSM was a great optimizing tool for extraction of gelatin from pangasius catfish skin. The gelatin was also proven to have significantly (p<0.05) higher quality of physicochemical properties than those from bovine skin gelatin. Universiti Kebangsaan Malaysia 2014-07 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/7254/1/05_Fatemeh.pdf Mahmoodani, Fatemeh and Ardekani, Venus Sanaei and See, Siau Fern and Salma Mohamad Yusop, and Abdul Salam Babji, (2014) Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin. Sains Malaysiana, 43 (7). pp. 995-1002. ISSN 0126-6039 http://www.ukm.my/jsm/
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	In order to optimize the extraction of gelatin from pangasius catfish skin, a response surface method (RSM) involving a Central Composite Design (CCD) was applied. Four variables, namely NaOH concentration (0-0.3 N), acetic acid concentration (0.025-0.125 N), extraction time (2-4 h) and extraction temperature (40-80°C) were selected as independent variables for the optimization using RSM. The dependent variable was calculated by hydroxyproline recovery. The optimum conditions for extraction were produced by a pre-treatment of 0.2 N NaOH and 0.1 N acetic acid along with hot water extraction at 63.7°C for 2.41 h. The results showed that the predicted response by RSM (68.53%) closely matched the experimental response of 68.16%. The results indicated that the extracted gelatin possessed high gel strength (438 g) and high content of imino acid (proline and hydroxyproline) (18.01%) with a viscosity of 4.67 mPa s. The results showed that RSM was a great optimizing tool for extraction of gelatin from pangasius catfish skin. The gelatin was also proven to have significantly (p<0.05) higher quality of physicochemical properties than those from bovine skin gelatin.
format	Article
author	Mahmoodani, Fatemeh Ardekani, Venus Sanaei See, Siau Fern Salma Mohamad Yusop, Abdul Salam Babji,
spellingShingle	Mahmoodani, Fatemeh Ardekani, Venus Sanaei See, Siau Fern Salma Mohamad Yusop, Abdul Salam Babji, Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin
author_facet	Mahmoodani, Fatemeh Ardekani, Venus Sanaei See, Siau Fern Salma Mohamad Yusop, Abdul Salam Babji,
author_sort	Mahmoodani, Fatemeh
title	Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin
title_short	Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin
title_full	Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin
title_fullStr	Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin
title_full_unstemmed	Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin
title_sort	optimization of extraction and physicochemical properties of gelatin from pangasius catfish (pangasius sutchi) skin
publisher	Universiti Kebangsaan Malaysia
publishDate	2014
url	http://journalarticle.ukm.my/7254/ http://journalarticle.ukm.my/7254/ http://journalarticle.ukm.my/7254/1/05_Fatemeh.pdf
first_indexed	2023-09-18T19:49:10Z
last_indexed	2023-09-18T19:49:10Z
_version_	1777406103312662528

Optimization of extraction and physicochemical properties of gelatin from pangasius catfish (Pangasius sutchi) skin

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