Responses of different strains of saccharomyces cerevisiae to osmotic stress

During fermentation cells are subjected to various kinds of stress. One of the stresses concerned is high osmotic environment, which cells need to encounter in order to continue growing. To understand how cells adapt to this stress condition, information from genome, proteome and metabolome levels a...

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Main Authors:	Noorhisham Tan Kofli, Keisuke Nagahisa, Suteaki Shioya
Format:	Article
Published:	Universiti Kebangsaan Malaysia 2006
Online Access:	http://journalarticle.ukm.my/3983/ http://journalarticle.ukm.my/3983/

id	ukm-3983
recordtype	eprints
spelling	ukm-39832012-03-28T03:16:11Z http://journalarticle.ukm.my/3983/ Responses of different strains of saccharomyces cerevisiae to osmotic stress Noorhisham Tan Kofli, Keisuke Nagahisa, Suteaki Shioya, During fermentation cells are subjected to various kinds of stress. One of the stresses concerned is high osmotic environment, which cells need to encounter in order to continue growing. To understand how cells adapt to this stress condition, information from genome, proteome and metabolome levels are crucial. In yeast cells, it was report that they produce glycerol to avoid depletion of water in the cell that could lead to cell shrinkage and eventually death. Thus, investigation of physiological responses were executed by shake flask method using three different Saccharomyces cerevisiae strains namely s288c, IFO2347 and FY834 which were grown in yeast potato dextrose (YPD) medium under the treatment of sodium chloride (NaCl) and sorbitol at 1M concentration to create the osmotic condition. These agents were added into the medium after 5 hours of fermentation when the cells reached exponential phase and carbon source is still available. The results proved that addition of both NaCl and sorbitol created the osmotic condition during growth resulted in higher accumulation of glycerol and trehalose when compared to the control in all strains. Among these strains, production of glycerol (g glycerol/g cell dry weight) was found highest in IFO2347, followed by s288c and FY834. Universiti Kebangsaan Malaysia 2006-12 Article PeerReviewed Noorhisham Tan Kofli, and Keisuke Nagahisa, and Suteaki Shioya, (2006) Responses of different strains of saccharomyces cerevisiae to osmotic stress. Sains Malaysiana, 35 (2). pp. 9-15. ISSN 0126-6039 http://www.ukm.my/jsm/english_journals/vol35num2_2006/vol35num2_06page9-15.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
description	During fermentation cells are subjected to various kinds of stress. One of the stresses concerned is high osmotic environment, which cells need to encounter in order to continue growing. To understand how cells adapt to this stress condition, information from genome, proteome and metabolome levels are crucial. In yeast cells, it was report that they produce glycerol to avoid depletion of water in the cell that could lead to cell shrinkage and eventually death. Thus, investigation of physiological responses were executed by shake flask method using three different Saccharomyces cerevisiae strains namely s288c, IFO2347 and FY834 which were grown in yeast potato dextrose (YPD) medium under the treatment of sodium chloride (NaCl) and sorbitol at 1M concentration to create the osmotic condition. These agents were added into the medium after 5 hours of fermentation when the cells reached exponential phase and carbon source is still available. The results proved that addition of both NaCl and sorbitol created the osmotic condition during growth resulted in higher accumulation of glycerol and trehalose when compared to the control in all strains. Among these strains, production of glycerol (g glycerol/g cell dry weight) was found highest in IFO2347, followed by s288c and FY834.
format	Article
author	Noorhisham Tan Kofli, Keisuke Nagahisa, Suteaki Shioya,
spellingShingle	Noorhisham Tan Kofli, Keisuke Nagahisa, Suteaki Shioya, Responses of different strains of saccharomyces cerevisiae to osmotic stress
author_facet	Noorhisham Tan Kofli, Keisuke Nagahisa, Suteaki Shioya,
author_sort	Noorhisham Tan Kofli,
title	Responses of different strains of saccharomyces cerevisiae to osmotic stress
title_short	Responses of different strains of saccharomyces cerevisiae to osmotic stress
title_full	Responses of different strains of saccharomyces cerevisiae to osmotic stress
title_fullStr	Responses of different strains of saccharomyces cerevisiae to osmotic stress
title_full_unstemmed	Responses of different strains of saccharomyces cerevisiae to osmotic stress
title_sort	responses of different strains of saccharomyces cerevisiae to osmotic stress
publisher	Universiti Kebangsaan Malaysia
publishDate	2006
url	http://journalarticle.ukm.my/3983/ http://journalarticle.ukm.my/3983/
first_indexed	2023-09-18T19:40:19Z
last_indexed	2023-09-18T19:40:19Z
_version_	1777405546157047808

Responses of different strains of saccharomyces cerevisiae to osmotic stress

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