Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology

Optimisation is commonly employed to find the conditions that produce the best possible response, thus minimizing the cost and time of a particular process. Response Surface Methodology (RSM) is a widely used tool in optimisation. This study reported the optimisation of enzymatic hydrolysis of mud...

Full description

Bibliographic Details
Main Authors:	Zaliha Harun, Amiza Mat Amin, Norizah Mhd Sarbon, Mohamad Khairi Mohd Zainol
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/12357/ http://journalarticle.ukm.my/12357/ http://journalarticle.ukm.my/12357/1/46_03_05.pdf

id	ukm-12357
recordtype	eprints
spelling	ukm-123572018-11-30T10:52:59Z http://journalarticle.ukm.my/12357/ Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology Zaliha Harun, Amiza Mat Amin, Norizah Mhd Sarbon, Mohamad Khairi Mohd Zainol, Optimisation is commonly employed to find the conditions that produce the best possible response, thus minimizing the cost and time of a particular process. Response Surface Methodology (RSM) is a widely used tool in optimisation. This study reported the optimisation of enzymatic hydrolysis of mud crab meat using RSM by employing a face-centered Central Composite Design (CCD) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity. Screening of commercial food grade proteinases was carried out using Alcalase®, Protamex®, Neutrase® and papain shows that Protamex® gave the highest ACEI activity. The enzymatic hydrolysis conditions used in the optimisation were temperature (45-65ºC), pH (pH 5.5-7.5), hydrolysis time (1-4 hours) and Protamex® enzyme to substrate (E/S) ratio (1-3%). It was found that quadratic model was able to explain the relationship between the hydrolysis conditions and ACEI activity. The optimum conditions were obtained at 65ºC, pH 5.6, 1% E/S ratio and 4 hours of hydrolysis time. The experimental ACEI activity (88.93±1.02%) was not significantly difference (p>0.05) with the predicted ACEI activity (91.10%). It was found that the IC50 of the mud crab hydrolysate prepared at optimum condition was 1.96±0.13 mg/mL. This study shows that RSM can be used to explain the relationship between enzymatic hydrolysis conditions of mud crab meat and its ACEI activity. Penerbit Universiti Kebangsaan Malaysia 2017-10 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/12357/1/46_03_05.pdf Zaliha Harun, and Amiza Mat Amin, and Norizah Mhd Sarbon, and Mohamad Khairi Mohd Zainol, (2017) Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology. Malaysian Applied Biology, 46 (3). pp. 33-40. ISSN 0126-8643 http://mabjournal.com/index.php?option=com_content&view=article&id=674&catid=59:current-view&Itemid=56
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Optimisation is commonly employed to find the conditions that produce the best possible response, thus minimizing the cost and time of a particular process. Response Surface Methodology (RSM) is a widely used tool in optimisation. This study reported the optimisation of enzymatic hydrolysis of mud crab meat using RSM by employing a face-centered Central Composite Design (CCD) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity. Screening of commercial food grade proteinases was carried out using Alcalase®, Protamex®, Neutrase® and papain shows that Protamex® gave the highest ACEI activity. The enzymatic hydrolysis conditions used in the optimisation were temperature (45-65ºC), pH (pH 5.5-7.5), hydrolysis time (1-4 hours) and Protamex® enzyme to substrate (E/S) ratio (1-3%). It was found that quadratic model was able to explain the relationship between the hydrolysis conditions and ACEI activity. The optimum conditions were obtained at 65ºC, pH 5.6, 1% E/S ratio and 4 hours of hydrolysis time. The experimental ACEI activity (88.93±1.02%) was not significantly difference (p>0.05) with the predicted ACEI activity (91.10%). It was found that the IC50 of the mud crab hydrolysate prepared at optimum condition was 1.96±0.13 mg/mL. This study shows that RSM can be used to explain the relationship between enzymatic hydrolysis conditions of mud crab meat and its ACEI activity.
format	Article
author	Zaliha Harun, Amiza Mat Amin, Norizah Mhd Sarbon, Mohamad Khairi Mohd Zainol,
spellingShingle	Zaliha Harun, Amiza Mat Amin, Norizah Mhd Sarbon, Mohamad Khairi Mohd Zainol, Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology
author_facet	Zaliha Harun, Amiza Mat Amin, Norizah Mhd Sarbon, Mohamad Khairi Mohd Zainol,
author_sort	Zaliha Harun,
title	Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology
title_short	Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology
title_full	Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology
title_fullStr	Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology
title_full_unstemmed	Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology
title_sort	optimisation of enzymatic protein hydrolysis of mud crab (scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (acei) activity using response surface methodology
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/12357/ http://journalarticle.ukm.my/12357/ http://journalarticle.ukm.my/12357/1/46_03_05.pdf
first_indexed	2023-09-18T20:02:25Z
last_indexed	2023-09-18T20:02:25Z
_version_	1777406937225232384

Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology

Similar Items