Optimisation of enzymatic protein hydrolysis of mud crab (Scylla sp.) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity using response surface methodology
Optimisation is commonly employed to find the conditions that produce the best possible response, thus minimizing the cost and time of a particular process. Response Surface Methodology (RSM) is a widely used tool in optimisation. This study reported the optimisation of enzymatic hydrolysis of mud...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2017
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| Online Access: | http://journalarticle.ukm.my/12357/ http://journalarticle.ukm.my/12357/ http://journalarticle.ukm.my/12357/1/46_03_05.pdf |
| Summary: | Optimisation is commonly employed to find the conditions that produce the best possible response, thus minimizing the cost
and time of a particular process. Response Surface Methodology (RSM) is a widely used tool in optimisation. This study
reported the optimisation of enzymatic hydrolysis of mud crab meat using RSM by employing a face-centered Central
Composite Design (CCD) to obtain maximum angiotensin-converting enzyme inhibitory (ACEI) activity. Screening of
commercial food grade proteinases was carried out using Alcalase®, Protamex®, Neutrase® and papain shows that Protamex®
gave the highest ACEI activity. The enzymatic hydrolysis conditions used in the optimisation were temperature (45-65ºC),
pH (pH 5.5-7.5), hydrolysis time (1-4 hours) and Protamex® enzyme to substrate (E/S) ratio (1-3%). It was found that
quadratic model was able to explain the relationship between the hydrolysis conditions and ACEI activity. The optimum
conditions were obtained at 65ºC, pH 5.6, 1% E/S ratio and 4 hours of hydrolysis time. The experimental ACEI activity
(88.93±1.02%) was not significantly difference (p>0.05) with the predicted ACEI activity (91.10%). It was found that the
IC50 of the mud crab hydrolysate prepared at optimum condition was 1.96±0.13 mg/mL. This study shows that RSM can be
used to explain the relationship between enzymatic hydrolysis conditions of mud crab meat and its ACEI activity. |
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