Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts

Quassia borneensis has been traditionally used as antihypertensive agent without any scientific literature on its mechanism of action. The objective of this study was to evaluate the antiinflammatory, antioxidant and antiproliferation properties of Q. borneensis extracts. The hexane, chloroform and...

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Main Authors:	Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim, Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali
Format:	Article
Language:	English
Published:	Penerbit Universiti Kebangsaan Malaysia 2017
Online Access:	http://journalarticle.ukm.my/11456/ http://journalarticle.ukm.my/11456/ http://journalarticle.ukm.my/11456/1/16743-47128-1-SM.pdf

id	ukm-11456
recordtype	eprints
spelling	ukm-114562018-03-14T10:01:40Z http://journalarticle.ukm.my/11456/ Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali, Quassia borneensis has been traditionally used as antihypertensive agent without any scientific literature on its mechanism of action. The objective of this study was to evaluate the antiinflammatory, antioxidant and antiproliferation properties of Q. borneensis extracts. The hexane, chloroform and aqueous extracts of root and bark of Q. borneensis were subjected to nitric oxide (NO) inhibition assay in LPS-stimulated RAW 264.7 cells. Expression of inducible NO synthase (iNOS) protein level was analyzed by Western blot. The antioxidant and antiproliferative activities of the extracts on HL-60 cells were determined using Ferric Reducing Antioxidant Power (FRAP) and MTT assays, respectively. The chloroform extract of Q. borneensis root obtained by soxhlet method (CSR) significantly inhibited 97.64 ± 0.96% of NO production (p < 0.001) and suppressed iNOS expression (p < 0.05) at the highest concentration of 1.0 μg/ml. The chloroform extract of bark obtained by maceration (CMB) exhibited the highest antioxidant capacity in the absence and presence of HL-60 cells, where the FRAP value were 125.45 ± 9.10 μM FeSO4.7H2O and 181.55 ± 3.45 μM FeSO4.7H2O, respectively. The greatest inhibition of HL-60 cell proliferation was exhibited by the chloroform extract of bark obtained by soxhlet method (CSB) with the IC50 of 5.0 μg/ml. The findings suggested that the chloroform extracts of Q. borneensis possess antiinflammatory, antioxidant and antiproliferative activities. Penerbit Universiti Kebangsaan Malaysia 2017 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/11456/1/16743-47128-1-SM.pdf Firdaus Kamarulzaman, and Julenah Ag Nuddin, and Kai, Li Lim and Aishah Adam, and Ahmad Sazali Hamzah, and Ahmad Rohi Ghazali, (2017) Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts. Jurnal Sains Kesihatan Malaysia, 15 (1). pp. 33-40. ISSN 1675-8161 http://ejournal.ukm.my/jskm/issue/view/635
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Kebangasaan Malaysia
building	UKM Institutional Repository
collection	Online Access
language	English
description	Quassia borneensis has been traditionally used as antihypertensive agent without any scientific literature on its mechanism of action. The objective of this study was to evaluate the antiinflammatory, antioxidant and antiproliferation properties of Q. borneensis extracts. The hexane, chloroform and aqueous extracts of root and bark of Q. borneensis were subjected to nitric oxide (NO) inhibition assay in LPS-stimulated RAW 264.7 cells. Expression of inducible NO synthase (iNOS) protein level was analyzed by Western blot. The antioxidant and antiproliferative activities of the extracts on HL-60 cells were determined using Ferric Reducing Antioxidant Power (FRAP) and MTT assays, respectively. The chloroform extract of Q. borneensis root obtained by soxhlet method (CSR) significantly inhibited 97.64 ± 0.96% of NO production (p < 0.001) and suppressed iNOS expression (p < 0.05) at the highest concentration of 1.0 μg/ml. The chloroform extract of bark obtained by maceration (CMB) exhibited the highest antioxidant capacity in the absence and presence of HL-60 cells, where the FRAP value were 125.45 ± 9.10 μM FeSO4.7H2O and 181.55 ± 3.45 μM FeSO4.7H2O, respectively. The greatest inhibition of HL-60 cell proliferation was exhibited by the chloroform extract of bark obtained by soxhlet method (CSB) with the IC50 of 5.0 μg/ml. The findings suggested that the chloroform extracts of Q. borneensis possess antiinflammatory, antioxidant and antiproliferative activities.
format	Article
author	Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali,
spellingShingle	Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali, Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts
author_facet	Firdaus Kamarulzaman, Julenah Ag Nuddin, Kai, Li Lim Aishah Adam, Ahmad Sazali Hamzah, Ahmad Rohi Ghazali,
author_sort	Firdaus Kamarulzaman,
title	Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts
title_short	Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts
title_full	Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts
title_fullStr	Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts
title_full_unstemmed	Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts
title_sort	evaluation of antiinflammatory, antioxidant and antiproliferative activities of quassia borneensis noot. (simaroubaceae) extracts
publisher	Penerbit Universiti Kebangsaan Malaysia
publishDate	2017
url	http://journalarticle.ukm.my/11456/ http://journalarticle.ukm.my/11456/ http://journalarticle.ukm.my/11456/1/16743-47128-1-SM.pdf
first_indexed	2023-09-18T20:00:24Z
last_indexed	2023-09-18T20:00:24Z
_version_	1777406809800179712

Evaluation of antiinflammatory, antioxidant and antiproliferative activities of Quassia borneensis Noot. (Simaroubaceae) extracts

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