Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli

Breast cancer is a common disease and one of the leading causes of cancer death among women in Malaysia. Indeed, in spite of the continual research for the development of this fatal disease, the effort is hampered due to lack of understanding on the pathophysiological of the heterogenous nature of b...

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Main Author: Rosli, Erda Syerena
Format: Thesis
Language:English
Published: 2015
Subjects:
Online Access:http://ir.uitm.edu.my/id/eprint/15635/
http://ir.uitm.edu.my/id/eprint/15635/1/TM_ERDA%20SYERENA%20ROSLI%20PH%2015_5.pdf
id uitm-15635
recordtype eprints
spelling uitm-156352016-12-18T09:50:22Z http://ir.uitm.edu.my/id/eprint/15635/ Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli Rosli, Erda Syerena Cancer Breast cancer is a common disease and one of the leading causes of cancer death among women in Malaysia. Indeed, in spite of the continual research for the development of this fatal disease, the effort is hampered due to lack of understanding on the pathophysiological of the heterogenous nature of breast cancer. Therefore, metabolomics platform has been used to study the alterations of metabolic pathways which are involved in the disease state. This study aimed to discover the metabolic profiles as well as some potential biomarkers of breast cancer patients in different stages of the disease. Hence, global metabolite profiling was used. Plasma samples of 22 breast cancer patients and healthy volunteers were compared. In addition, tumour tissue and normal tissue of 12 breast cancer patients were analysed. Samples were subjected to Liquid Chromatography Mass Spectrometry Time of Flight (LCMS/QTOF). Method optimization using different extraction solvents was performed. Significant analysis (corrected p-value, p<0.05) and fold change analysis (FC^2.0) were done. All detected metabolites that have passed through applicable statistical methods were treated as potential biomarkers and were used for differential analysis. In the comparison between breast cancer patients and healthy volunteers, maltose and hypoxanthine which are involved in energy production and deoxyribonucleic acid (DNA) synthesis pathway were reported with the highest AUC value of 0.941 and 0.898, respectively. On the other hand, most fatty acids and lipid metabolites were significantly perturbated in tumour samples when compared to normal tissue samples. This relates to their importance in proliferation of cancer cells. Meanwhile, based on the patient’s estrogen receptor (ER) status, xanthine and hypoxanthine were observed to be down-regulated in ER negative tumour tissues compared to ER positive. On the other hand, spermine was up-regulated in ER negative tumour tissues. In tumour tissue grading status results, eicosanoid metabolites and spermine were found to be significantly different. From the finding, disruption of spermine in ER status and grading status of tumour tissues can be subjected to the degree of aggressiveness in tumour progression. Based on the results, we believed that these “potential biomarkers” can possibly be used as biomarkers for further study to improve the diagnosis and therapeutic strategy for breast cancer. 2015 Thesis NonPeerReviewed text en http://ir.uitm.edu.my/id/eprint/15635/1/TM_ERDA%20SYERENA%20ROSLI%20PH%2015_5.pdf Rosli, Erda Syerena (2015) Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli. Masters thesis, Universiti Teknologi MARA.
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Cancer
spellingShingle Cancer
Rosli, Erda Syerena
Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli
description Breast cancer is a common disease and one of the leading causes of cancer death among women in Malaysia. Indeed, in spite of the continual research for the development of this fatal disease, the effort is hampered due to lack of understanding on the pathophysiological of the heterogenous nature of breast cancer. Therefore, metabolomics platform has been used to study the alterations of metabolic pathways which are involved in the disease state. This study aimed to discover the metabolic profiles as well as some potential biomarkers of breast cancer patients in different stages of the disease. Hence, global metabolite profiling was used. Plasma samples of 22 breast cancer patients and healthy volunteers were compared. In addition, tumour tissue and normal tissue of 12 breast cancer patients were analysed. Samples were subjected to Liquid Chromatography Mass Spectrometry Time of Flight (LCMS/QTOF). Method optimization using different extraction solvents was performed. Significant analysis (corrected p-value, p<0.05) and fold change analysis (FC^2.0) were done. All detected metabolites that have passed through applicable statistical methods were treated as potential biomarkers and were used for differential analysis. In the comparison between breast cancer patients and healthy volunteers, maltose and hypoxanthine which are involved in energy production and deoxyribonucleic acid (DNA) synthesis pathway were reported with the highest AUC value of 0.941 and 0.898, respectively. On the other hand, most fatty acids and lipid metabolites were significantly perturbated in tumour samples when compared to normal tissue samples. This relates to their importance in proliferation of cancer cells. Meanwhile, based on the patient’s estrogen receptor (ER) status, xanthine and hypoxanthine were observed to be down-regulated in ER negative tumour tissues compared to ER positive. On the other hand, spermine was up-regulated in ER negative tumour tissues. In tumour tissue grading status results, eicosanoid metabolites and spermine were found to be significantly different. From the finding, disruption of spermine in ER status and grading status of tumour tissues can be subjected to the degree of aggressiveness in tumour progression. Based on the results, we believed that these “potential biomarkers” can possibly be used as biomarkers for further study to improve the diagnosis and therapeutic strategy for breast cancer.
format Thesis
author Rosli, Erda Syerena
author_facet Rosli, Erda Syerena
author_sort Rosli, Erda Syerena
title Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli
title_short Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli
title_full Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli
title_fullStr Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli
title_full_unstemmed Comparative metabolic changes of tissues and plasma in breast cancer patients / Erda Syerena Rosli
title_sort comparative metabolic changes of tissues and plasma in breast cancer patients / erda syerena rosli
publishDate 2015
url http://ir.uitm.edu.my/id/eprint/15635/
http://ir.uitm.edu.my/id/eprint/15635/1/TM_ERDA%20SYERENA%20ROSLI%20PH%2015_5.pdf
first_indexed 2023-09-18T22:54:18Z
last_indexed 2023-09-18T22:54:18Z
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