Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin

Proteases were extracted from starfruit at maturity Index 2 (unripe, light green) and Index 7 (very ripe, orange) and partially purified using acetone and 40% ammonium sulfate precipitations. Higher yield and proteolytic activity were observed for proteases purified using acetone than 40% ammonium...

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Main Authors:	Ismail, Normah, Zainuddin, Ezzana Zuraini
Format:	Article
Language:	English
Published:	Institute of Research Management and Innovation (IRMI) 2015
Subjects:	Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate Acetone
Online Access:	http://ir.uitm.edu.my/id/eprint/13581/ http://ir.uitm.edu.my/id/eprint/13581/1/AJ_NORMAH%20ISMAIL%20SRJ%2015%201.pdf

id	uitm-13581
recordtype	eprints
spelling	uitm-135812016-05-30T05:07:44Z http://ir.uitm.edu.my/id/eprint/13581/ Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin Ismail, Normah Zainuddin, Ezzana Zuraini Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate Acetone Proteases were extracted from starfruit at maturity Index 2 (unripe, light green) and Index 7 (very ripe, orange) and partially purified using acetone and 40% ammonium sulfate precipitations. Higher yield and proteolytic activity were observed for proteases purified using acetone than 40% ammonium sulfate. As for maturity index, yield and protein concentration of proteases from Index 2 were higher than those from Index 7. SDS-PAGE result showed intense bands for acetone proteases while a distinct band at 50 kDa was observed in all the proteases. Enzyme activity decreased during the seven days storage at 4°C with minimum relative activity of 70%o achieved for acetone proteases at day seven. This study suggested that acetone precipitation is more effective method for purifying starfruit protease based on the yield and proteolytic activity compared to using 40%) ammonium sulphate precipitation. In order to obtain higher protein concentration and proteolytic activity, starfruit at the unripe stage, Index 2 is a better raw material than Index 7 to be used for protease production. Institute of Research Management and Innovation (IRMI) 2015 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/13581/1/AJ_NORMAH%20ISMAIL%20SRJ%2015%201.pdf Ismail, Normah and Zainuddin, Ezzana Zuraini (2015) Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin. Scientific Research Journal, 12 (1). pp. 11-23. ISSN 1675-7009
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Teknologi MARA
building	UiTM Institutional Repository
collection	Online Access
language	English
topic	Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate Acetone
spellingShingle	Oxalidaceae (Wood sorrel) Proteolytic enzymes. Proteases Ammonium sulfate Acetone Ismail, Normah Zainuddin, Ezzana Zuraini Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin
description	Proteases were extracted from starfruit at maturity Index 2 (unripe, light green) and Index 7 (very ripe, orange) and partially purified using acetone and 40% ammonium sulfate precipitations. Higher yield and proteolytic activity were observed for proteases purified using acetone than 40% ammonium sulfate. As for maturity index, yield and protein concentration of proteases from Index 2 were higher than those from Index 7. SDS-PAGE result showed intense bands for acetone proteases while a distinct band at 50 kDa was observed in all the proteases. Enzyme activity decreased during the seven days storage at 4°C with minimum relative activity of 70%o achieved for acetone proteases at day seven. This study suggested that acetone precipitation is more effective method for purifying starfruit protease based on the yield and proteolytic activity compared to using 40%) ammonium sulphate precipitation. In order to obtain higher protein concentration and proteolytic activity, starfruit at the unripe stage, Index 2 is a better raw material than Index 7 to be used for protease production.
format	Article
author	Ismail, Normah Zainuddin, Ezzana Zuraini
author_facet	Ismail, Normah Zainuddin, Ezzana Zuraini
author_sort	Ismail, Normah
title	Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin
title_short	Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin
title_full	Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin
title_fullStr	Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin
title_full_unstemmed	Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin
title_sort	effects of precipitation methods on the properties of protease extracted from starfruit (averrhoa carambola l.) of different maturity index / normah ismail and ezzana zuraini zainuddin
publisher	Institute of Research Management and Innovation (IRMI)
publishDate	2015
url	http://ir.uitm.edu.my/id/eprint/13581/ http://ir.uitm.edu.my/id/eprint/13581/1/AJ_NORMAH%20ISMAIL%20SRJ%2015%201.pdf
first_indexed	2023-09-18T22:50:03Z
last_indexed	2023-09-18T22:50:03Z
_version_	1777417483476533248

Effects of precipitation methods on the properties of protease extracted from starfruit (Averrhoa carambola L.) of different maturity index / Normah Ismail and Ezzana Zuraini Zainuddin

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