Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal

In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of 0.5 and 3% using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties s...

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Main Authors: Ismail, Normah, Mahmod, Juliana, Mustafa Kamal, Awatif Khairul Fatihin
Format: Article
Language:English
Published: Research Management Institute (RMI) 2014
Subjects:
Online Access:http://ir.uitm.edu.my/id/eprint/12794/
http://ir.uitm.edu.my/id/eprint/12794/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2014.pdf
id uitm-12794
recordtype eprints
spelling uitm-127942016-05-26T03:06:01Z http://ir.uitm.edu.my/id/eprint/12794/ Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal Ismail, Normah Mahmod, Juliana Mustafa Kamal, Awatif Khairul Fatihin Physical and theoretical chemistry Clams In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of 0.5 and 3% using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties such as solubility, emulsifying properties and water and oil holding capacity. Degree of hydrolysis (DH), yield, functional and antioxidant properties were influenced by the hydrolysis time and E/S ratio. Higher enzyme concentration (E/S 3%) and longer hydrolysis time increased the DH. Yield was higher at E/S 3% but reduced with hydrolysis time. Longer hydrolysis time produced more soluble hydrolysate and higher metal chelating activity but lower in emulsifying properties and DPPH activity. Higher enzyme concentration resulted in increase only in solubility and metal chelating activity. This study revealed that enzymatic hydrolysis using alcalase should be performed at shorter hydrolysis time using intermediate concentration of enzyme (E/S between 0.5 to 3%) in order to produce angelwing clam hydrolysate with collectively good functional and antioxidant properties. Research Management Institute (RMI) 2014 Article PeerReviewed text en http://ir.uitm.edu.my/id/eprint/12794/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2014.pdf Ismail, Normah and Mahmod, Juliana and Mustafa Kamal, Awatif Khairul Fatihin (2014) Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal. Scientific Research Journal, 11 (1). pp. 29-49. ISSN 1675-7009
repository_type Digital Repository
institution_category Local University
institution Universiti Teknologi MARA
building UiTM Institutional Repository
collection Online Access
language English
topic Physical and theoretical chemistry
Clams
spellingShingle Physical and theoretical chemistry
Clams
Ismail, Normah
Mahmod, Juliana
Mustafa Kamal, Awatif Khairul Fatihin
Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
description In this study, Hydrolysate from angelwing clam (Pholas orientalis) was produced at 0, 1, 2 and 3 hrs and E/S ratio of 0.5 and 3% using alcalase where the pH and temperature were kept constant at pH 8.5 and 60°C, respectively. The hydrolysates were analysed for antioxidant and functional properties such as solubility, emulsifying properties and water and oil holding capacity. Degree of hydrolysis (DH), yield, functional and antioxidant properties were influenced by the hydrolysis time and E/S ratio. Higher enzyme concentration (E/S 3%) and longer hydrolysis time increased the DH. Yield was higher at E/S 3% but reduced with hydrolysis time. Longer hydrolysis time produced more soluble hydrolysate and higher metal chelating activity but lower in emulsifying properties and DPPH activity. Higher enzyme concentration resulted in increase only in solubility and metal chelating activity. This study revealed that enzymatic hydrolysis using alcalase should be performed at shorter hydrolysis time using intermediate concentration of enzyme (E/S between 0.5 to 3%) in order to produce angelwing clam hydrolysate with collectively good functional and antioxidant properties.
format Article
author Ismail, Normah
Mahmod, Juliana
Mustafa Kamal, Awatif Khairul Fatihin
author_facet Ismail, Normah
Mahmod, Juliana
Mustafa Kamal, Awatif Khairul Fatihin
author_sort Ismail, Normah
title Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_short Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_full Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_fullStr Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_full_unstemmed Functional and antioxidant properties of angelwing clam (Pholas Orientalis) hydrolysate produced using alcalase / Normah Ismail, Juliana Mahmod and Awatif Khairul Fatihin Mustafa Kamal
title_sort functional and antioxidant properties of angelwing clam (pholas orientalis) hydrolysate produced using alcalase / normah ismail, juliana mahmod and awatif khairul fatihin mustafa kamal
publisher Research Management Institute (RMI)
publishDate 2014
url http://ir.uitm.edu.my/id/eprint/12794/
http://ir.uitm.edu.my/id/eprint/12794/1/AJ_NORMAH%20ISMAIL%20%282%29%20SRJ%2014.pdf
first_indexed 2023-09-18T22:49:26Z
last_indexed 2023-09-18T22:49:26Z
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