Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC)

Xanthine oxidase (XO) is an enzyme that catalyses the metabolism of hypoxanthine and xanthine into uric acid. It is responsible for the medical condition known as gout, which is caused by the deposition of uric acid in the joints leading to painful inflammation. Inhibition of XO leads to remission i...

Full description

Bibliographic Details
Main Authors:	Azmi, Saiful Mohammad Nizam, Jamal, Parveen, Amid, Azura
Format:	Article
Language:	English
Published:	INSI Publications 2012
Subjects:	TA164 Bioengineering TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/9760/ http://irep.iium.edu.my/9760/ http://irep.iium.edu.my/9760/4/117-122_9760.pdf

id	iium-9760
recordtype	eprints
spelling	iium-97602012-06-15T01:58:05Z http://irep.iium.edu.my/9760/ Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC) Azmi, Saiful Mohammad Nizam Jamal, Parveen Amid, Azura TA164 Bioengineering TP248.13 Biotechnology Xanthine oxidase (XO) is an enzyme that catalyses the metabolism of hypoxanthine and xanthine into uric acid. It is responsible for the medical condition known as gout, which is caused by the deposition of uric acid in the joints leading to painful inflammation. Inhibition of XO leads to remission in gout. Carica papaya is a member of the small family Caricaceae allied to the Passifloraceae. In folk medicine, Carica papaya has been used as an important traditional herbal medicine due to its vast bioactive compounds found including kaempferol, quercetin, 5, 7-dimethoxycoumarin, alkaloids, carpaine and pseudocarpaine. In this study, the dried mature leaves of Carica papaya was extracted with distilled water, optimized to obtain higher XO inhibitory activity, and the extract was subjected to reversed-phase flash column chromatography (RPFCC) and high performance thin layer chromatography (HPTLC) for the purification. Preliminary identification by chemical screening conducted on the optimized sample of distilled water extract of Carica papaya leaves showed the presence of several secondary metabolites, mainly, flavonoids, alkaloids, tannins, saponins and xanthine alkaloids, terpenoids and glycosides which could partially explain the pharmacological properties of this plant and demonstrates its importance in alimentation and daily intake especially for gout patient. INSI Publications 2012-01 Article PeerReviewed application/pdf en http://irep.iium.edu.my/9760/4/117-122_9760.pdf Azmi, Saiful Mohammad Nizam and Jamal, Parveen and Amid, Azura (2012) Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC). Australian Journal of Basic and Applied Sciences, 6 (1). pp. 117-122. ISSN 1991-8178 http://www.insipub.com/ajbas/2012/January/117-122.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	TA164 Bioengineering TP248.13 Biotechnology
spellingShingle	TA164 Bioengineering TP248.13 Biotechnology Azmi, Saiful Mohammad Nizam Jamal, Parveen Amid, Azura Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC)
description	Xanthine oxidase (XO) is an enzyme that catalyses the metabolism of hypoxanthine and xanthine into uric acid. It is responsible for the medical condition known as gout, which is caused by the deposition of uric acid in the joints leading to painful inflammation. Inhibition of XO leads to remission in gout. Carica papaya is a member of the small family Caricaceae allied to the Passifloraceae. In folk medicine, Carica papaya has been used as an important traditional herbal medicine due to its vast bioactive compounds found including kaempferol, quercetin, 5, 7-dimethoxycoumarin, alkaloids, carpaine and pseudocarpaine. In this study, the dried mature leaves of Carica papaya was extracted with distilled water, optimized to obtain higher XO inhibitory activity, and the extract was subjected to reversed-phase flash column chromatography (RPFCC) and high performance thin layer chromatography (HPTLC) for the purification. Preliminary identification by chemical screening conducted on the optimized sample of distilled water extract of Carica papaya leaves showed the presence of several secondary metabolites, mainly, flavonoids, alkaloids, tannins, saponins and xanthine alkaloids, terpenoids and glycosides which could partially explain the pharmacological properties of this plant and demonstrates its importance in alimentation and daily intake especially for gout patient.
format	Article
author	Azmi, Saiful Mohammad Nizam Jamal, Parveen Amid, Azura
author_facet	Azmi, Saiful Mohammad Nizam Jamal, Parveen Amid, Azura
author_sort	Azmi, Saiful Mohammad Nizam
title	Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC)
title_short	Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC)
title_full	Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC)
title_fullStr	Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC)
title_full_unstemmed	Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC)
title_sort	purification of xanthine oxidase inhibitor from carica papaya leaves using reversed phase flash column chromatography (rpfcc) - high performance thin layer chromatography (hptlc)
publisher	INSI Publications
publishDate	2012
url	http://irep.iium.edu.my/9760/ http://irep.iium.edu.my/9760/ http://irep.iium.edu.my/9760/4/117-122_9760.pdf
first_indexed	2023-09-18T20:19:22Z
last_indexed	2023-09-18T20:19:22Z
_version_	1777408003445620736

Purification of Xanthine Oxidase Inhibitor from Carica papaya leaves using Reversed Phase Flash Column Chromatography (RPFCC) - High Performance Thin Layer Chromatography (HPTLC)

Similar Items