Culturing and maintaining mammalian cell culture
Mammalian cell culture refers to culturing mammalian cells in a medium that provide nutrients for cells to be able to grow in vitro under environment that closely mimic the in vivo conditions. By enabling culturing these cells outside living biological entities, investigation on intra- and interc...
| Main Authors: | , , |
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| Format: | Book Chapter |
| Language: | English English |
| Published: |
Springer Nature
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70574/ http://irep.iium.edu.my/70574/ http://irep.iium.edu.my/70574/1/70574_Culturing%20and%20maintaining%20mammalian%20cell%20culture.pdf http://irep.iium.edu.my/70574/7/70574_Culturing%20and%20maintaining%20mammalian%20cell%20culture_Scopus.pdf |
| Summary: | Mammalian cell culture refers to culturing mammalian cells in a medium
that provide nutrients for cells to be able to grow in vitro under environment that
closely mimic the in vivo conditions. By enabling culturing these cells outside living
biological entities, investigation on intra- and intercellular activities and flux;
genetic and phenotyping analysis; proteomics, study of toxicology, drug discovery
and development can be carried out without manipulation of living animals. In this
chapter, detail protocol of media preparation, cell culture maintenance and preservation
are elaborated for both types of mammalian cell culture, monolayer or suspension
cultures. Determination of number of cells is discussed as well. |
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