Growth, substrate consumption, and product formation kinetics of Phanerochaete chrysosporium and Schizophyllum commune mixed culture under solid-state fermentation of fruit peels
Kinetic analysis of solid-state fermentation (SSF) of fruit peels with Phanerochaete chrysosporium and Schizophyllum commune mixed culture was studied in flask and 7 kg capacity reactor. Modified Monod kinetic model suggested by Haldane sufficiently described microbial growth with co-efficient of de...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Springer Nature
2018
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/70260/ http://irep.iium.edu.my/70260/ http://irep.iium.edu.my/70260/ http://irep.iium.edu.my/70260/1/70260_Growth%2C%20substrate%20consumption%2C%20and%20product%20formation_article.pdf http://irep.iium.edu.my/70260/2/70260_Growth%2C%20substrate%20consumption%2C%20and%20product%20formation_scopus.pdf http://irep.iium.edu.my/70260/13/70260%20Growth%2C%20substrate%20consumption%2C%20and%20product%20formation_wos.pdf |
| Summary: | Kinetic analysis of solid-state fermentation (SSF) of fruit peels with Phanerochaete chrysosporium and Schizophyllum commune mixed culture was studied in flask and 7 kg capacity reactor. Modified Monod kinetic model suggested by Haldane sufficiently described microbial growth with co-efficient of determination (R2) reaching 0.908 at increased substrate concentration than the classical Monod model (R2 = 0.932). Leudeking–Piret model adequately described product synthesis in non-growth-dependent manner (R2 = 0.989), while substrate consumption by P. chrysosporium and S. commune fungal mixed culture was growth-dependent (R2 = 0.938). Hanes–Woolf model sufficiently represented α-amylase and cellulase enzymes synthesis (R2 = 0.911 and 0.988); α-amylase had enzyme maximum velocity (Vmax) of 25.19 IU/gds/day and rate constant (Km) of 11.55 IU/gds/day, while cellulase enzyme had Vmax of 3.05 IU/gds/day and Km of 57.47 IU/gds/day. Product yield in the reactor increased to 32.65 mg/g/day compared with 28.15 mg/g/day in shake flask. 2.5 cm media thickness was adequate for product formation within a 6 day SSF in the tray reactor. |
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