Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis

Osteoarthritis (OA) is a degenerative joint diseases that progressed as a function of age. Previous studies have shown that the structural changes of osteoarthritic subchondral bone are associated with rapid bone remodelling, and differed between its two regions; subchondral bone plate (Sbp) and tra...

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Main Authors:	Akman, Akma Azmiera, Abdul Ghani, Radiah, Zamli, Zaitunnatakhin
Format:	Monograph
Language:	English
Published:	2018
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/66185/ http://irep.iium.edu.my/66185/1/Profile%20of%20Final%20Report%20RAGS%202015%20-%20Zaitunnatakhin%20Zamli.pdf

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recordtype	eprints
spelling	iium-661852018-10-27T15:02:37Z http://irep.iium.edu.my/66185/ Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis Akman, Akma Azmiera Abdul Ghani, Radiah Zamli, Zaitunnatakhin R Medicine (General) Osteoarthritis (OA) is a degenerative joint diseases that progressed as a function of age. Previous studies have shown that the structural changes of osteoarthritic subchondral bone are associated with rapid bone remodelling, and differed between its two regions; subchondral bone plate (Sbp) and trabecular bone (Tb). Despite of extensive studies in human and animal models of OA, the molecular mechanisms that underlying the differences between these two regions have yet to be determined. Hence, the present study aims to determine the structural changes and molecular mechanisms of Sbp and Tb during development of OA in Dunkin Hartley guinea pigs. To achieve these aims, the Sbp and Tb thickness of the tibia was determined by using a micro-computed tomography (micro-CT) at 10, 20 and 30 weeks of age. The level of bone resorption (i.e. Receptor activator of NF-κB ligand) and formation (i.e. osteoprotegerin) markers was quantified by using immunohistochemistry technique. The severity of the disease was microscopically scored by histopathology technique. The results showed that Sbp and Tb thickness were greater in the medial than the lateral side of tibial plateau, and increased significantly with ageing (p ≤ 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp was initially increased, before being decreased at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. The data also showed that the AC degeneration was more pronounced in the medial than the lateral side, and worsen with ageing. Interestingly, a strong correlation was observed between the Sbp thickness and OPG/RANKL ratio with microscopic OA scores (r = 0.5, p ≤ 0.05), suggesting that the Sbp plays an important role in the pathogenesis of OA. 2018-09 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/66185/1/Profile%20of%20Final%20Report%20RAGS%202015%20-%20Zaitunnatakhin%20Zamli.pdf Akman, Akma Azmiera and Abdul Ghani, Radiah and Zamli, Zaitunnatakhin (2018) Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis. Project Report. UNSPECIFIED. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Akman, Akma Azmiera Abdul Ghani, Radiah Zamli, Zaitunnatakhin Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis
description	Osteoarthritis (OA) is a degenerative joint diseases that progressed as a function of age. Previous studies have shown that the structural changes of osteoarthritic subchondral bone are associated with rapid bone remodelling, and differed between its two regions; subchondral bone plate (Sbp) and trabecular bone (Tb). Despite of extensive studies in human and animal models of OA, the molecular mechanisms that underlying the differences between these two regions have yet to be determined. Hence, the present study aims to determine the structural changes and molecular mechanisms of Sbp and Tb during development of OA in Dunkin Hartley guinea pigs. To achieve these aims, the Sbp and Tb thickness of the tibia was determined by using a micro-computed tomography (micro-CT) at 10, 20 and 30 weeks of age. The level of bone resorption (i.e. Receptor activator of NF-κB ligand) and formation (i.e. osteoprotegerin) markers was quantified by using immunohistochemistry technique. The severity of the disease was microscopically scored by histopathology technique. The results showed that Sbp and Tb thickness were greater in the medial than the lateral side of tibial plateau, and increased significantly with ageing (p ≤ 0.01). In addition, across the time points, the OPG/RANKL ratio of medial Sbp was initially increased, before being decreased at the final time point. In contrast, the OPG/RANKL ratio of medial Tb was initially constant but then decreased at 30 weeks of age. The data also showed that the AC degeneration was more pronounced in the medial than the lateral side, and worsen with ageing. Interestingly, a strong correlation was observed between the Sbp thickness and OPG/RANKL ratio with microscopic OA scores (r = 0.5, p ≤ 0.05), suggesting that the Sbp plays an important role in the pathogenesis of OA.
format	Monograph
author	Akman, Akma Azmiera Abdul Ghani, Radiah Zamli, Zaitunnatakhin
author_facet	Akman, Akma Azmiera Abdul Ghani, Radiah Zamli, Zaitunnatakhin
author_sort	Akman, Akma Azmiera
title	Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis
title_short	Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis
title_full	Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis
title_fullStr	Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis
title_full_unstemmed	Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis
title_sort	structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis
publishDate	2018
url	http://irep.iium.edu.my/66185/ http://irep.iium.edu.my/66185/1/Profile%20of%20Final%20Report%20RAGS%202015%20-%20Zaitunnatakhin%20Zamli.pdf
first_indexed	2023-09-18T21:33:55Z
last_indexed	2023-09-18T21:33:55Z
_version_	1777412694002892800

Structural changes and molecular mechanisms of bone remodelling in two different regions of subchondral bone during early osteoarthritis

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