Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems

This study evaluated the efficacy of various organic solvents (80% acetone, 80% ethanol, 80% methanol) and distilled water for extracting antioxidant phenolic compounds from turmeric, curry leaf, torch ginger and lemon grass extracts. They were analyzed regarding the total phenol and flavonoid co...

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Main Authors: Sepahpour, Shabnam, Selamat, Jinap, Abdul Manap, Mohd Yazid, Khatib, Alfi, Abdull Razis, Ahmad Faizal
Format: Article
Language:English
English
English
Published: Multidisciplinary Digital Publishing Institute (MDPI) 2018
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Online Access:http://irep.iium.edu.my/64187/
http://irep.iium.edu.my/64187/
http://irep.iium.edu.my/64187/
http://irep.iium.edu.my/64187/1/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_article.pdf
http://irep.iium.edu.my/64187/2/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_scopus.pdf
http://irep.iium.edu.my/64187/13/64187_Comparative%20analysis%20of%20chemical%20composition%2C%20antioxidant%20activity%20and%20quantitative%20characterization%20of%20_WoS.pdf
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spelling iium-641872018-08-01T01:16:51Z http://irep.iium.edu.my/64187/ Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems Sepahpour, Shabnam Selamat, Jinap Abdul Manap, Mohd Yazid Khatib, Alfi Abdull Razis, Ahmad Faizal RS192 Materia Medica-Pharmaceutical Technology TP Chemical technology This study evaluated the efficacy of various organic solvents (80% acetone, 80% ethanol, 80% methanol) and distilled water for extracting antioxidant phenolic compounds from turmeric, curry leaf, torch ginger and lemon grass extracts. They were analyzed regarding the total phenol and flavonoid contents, antioxidant activity and concentration of some phenolic compounds. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and the ferric reducing antioxidant power (FRAP) assay. Quantification of phenolic compounds was carried out using high-performance liquid chromatography (HPLC). All the extracts possessed antioxidant activity, however, the different solvents showed different efficiencies in the extraction of phenolic compounds. Turmeric showed the highest DPPH values (67.83–13.78%) and FRAP (84.9–2.3 mg quercetin/g freeze-dried crude extract), followed by curry leaf, torch ginger and lemon grass. While 80% acetone was shown to be the most efficient solvent for the extraction of total phenolic compounds from turmeric, torch ginger and lemon grass (221.68, 98.10 and 28.19 mg GA/g freeze dried crude extract, respectively), for the recovery of phenolic compounds from curry leaf (92.23 mg GA/g freeze-dried crude extract), 80% ethanol was the most appropriate solvent. Results of HPLC revealed that the amount of phenolic compounds varied depending on the types of solvents used. Multidisciplinary Digital Publishing Institute (MDPI) 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64187/1/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_article.pdf application/pdf en http://irep.iium.edu.my/64187/2/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_scopus.pdf application/pdf en http://irep.iium.edu.my/64187/13/64187_Comparative%20analysis%20of%20chemical%20composition%2C%20antioxidant%20activity%20and%20quantitative%20characterization%20of%20_WoS.pdf Sepahpour, Shabnam and Selamat, Jinap and Abdul Manap, Mohd Yazid and Khatib, Alfi and Abdull Razis, Ahmad Faizal (2018) Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems. Molecules, 23 (2). pp. 1-17. ISSN 1420-3049 http://www.mdpi.com/1420-3049/23/2/402 10.3390/molecules23020402
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
topic RS192 Materia Medica-Pharmaceutical Technology
TP Chemical technology
spellingShingle RS192 Materia Medica-Pharmaceutical Technology
TP Chemical technology
Sepahpour, Shabnam
Selamat, Jinap
Abdul Manap, Mohd Yazid
Khatib, Alfi
Abdull Razis, Ahmad Faizal
Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems
description This study evaluated the efficacy of various organic solvents (80% acetone, 80% ethanol, 80% methanol) and distilled water for extracting antioxidant phenolic compounds from turmeric, curry leaf, torch ginger and lemon grass extracts. They were analyzed regarding the total phenol and flavonoid contents, antioxidant activity and concentration of some phenolic compounds. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and the ferric reducing antioxidant power (FRAP) assay. Quantification of phenolic compounds was carried out using high-performance liquid chromatography (HPLC). All the extracts possessed antioxidant activity, however, the different solvents showed different efficiencies in the extraction of phenolic compounds. Turmeric showed the highest DPPH values (67.83–13.78%) and FRAP (84.9–2.3 mg quercetin/g freeze-dried crude extract), followed by curry leaf, torch ginger and lemon grass. While 80% acetone was shown to be the most efficient solvent for the extraction of total phenolic compounds from turmeric, torch ginger and lemon grass (221.68, 98.10 and 28.19 mg GA/g freeze dried crude extract, respectively), for the recovery of phenolic compounds from curry leaf (92.23 mg GA/g freeze-dried crude extract), 80% ethanol was the most appropriate solvent. Results of HPLC revealed that the amount of phenolic compounds varied depending on the types of solvents used.
format Article
author Sepahpour, Shabnam
Selamat, Jinap
Abdul Manap, Mohd Yazid
Khatib, Alfi
Abdull Razis, Ahmad Faizal
author_facet Sepahpour, Shabnam
Selamat, Jinap
Abdul Manap, Mohd Yazid
Khatib, Alfi
Abdull Razis, Ahmad Faizal
author_sort Sepahpour, Shabnam
title Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems
title_short Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems
title_full Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems
title_fullStr Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems
title_full_unstemmed Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems
title_sort comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems
publisher Multidisciplinary Digital Publishing Institute (MDPI)
publishDate 2018
url http://irep.iium.edu.my/64187/
http://irep.iium.edu.my/64187/
http://irep.iium.edu.my/64187/
http://irep.iium.edu.my/64187/1/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_article.pdf
http://irep.iium.edu.my/64187/2/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_scopus.pdf
http://irep.iium.edu.my/64187/13/64187_Comparative%20analysis%20of%20chemical%20composition%2C%20antioxidant%20activity%20and%20quantitative%20characterization%20of%20_WoS.pdf
first_indexed 2023-09-18T21:31:02Z
last_indexed 2023-09-18T21:31:02Z
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