Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food

This study was conducted to evaluate the severity of najs (filth) contamination in ready to eat food as to confirm the definition of halal food which suppose not contaminated with najs mutawwassitah (medium filth). A total of 52 human stools samples were collected and the screening of bacteria in th...

Full description

Bibliographic Details
Main Authors:	Abu Bakar, Ibrahim, Abdul Rafa, Ainin Azwani, Abdullah Sani, Norrakiah
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	QR Microbiology TP368 Food processing and manufacture
Online Access:	http://irep.iium.edu.my/63199/ http://irep.iium.edu.my/63199/ http://irep.iium.edu.my/63199/1/63199_MICROBIOLOGICAL%20GUIDELINES-abstract.pdf

id	iium-63199
recordtype	eprints
spelling	iium-631992018-04-09T02:01:05Z http://irep.iium.edu.my/63199/ Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food Abu Bakar, Ibrahim Abdul Rafa, Ainin Azwani Abdullah Sani, Norrakiah QR Microbiology TP368 Food processing and manufacture This study was conducted to evaluate the severity of najs (filth) contamination in ready to eat food as to confirm the definition of halal food which suppose not contaminated with najs mutawwassitah (medium filth). A total of 52 human stools samples were collected and the screening of bacteria in the human stools samples were done according to traditional microbiological analysis methods. Determinations of bacterial growth curves were performed using NanoDrop 1000 UV-VIS Spectrophotometer at 630nm where the initial and end of log times for each of bacteria was determined. The growth evaluation of fecal borne bacteria in ready to eat food was done using prepared fried rice samples which were prepared at food preparation laboratory. The results showed that healthy human stools which fall under najs mutawassitah contain high amount of presumptive pathogenic bacteria specifically E. coli, S. aureus, B. cereus, Aromonas spp. and Salmonella spp. at different mean values. Average lag phase time for faecal borne bacteria is about 60 minutes (one hour). Thus consuming food before one hour should not give any significant health effect. Consuming food which contains faecal borne bacteria within one to two hours will give either no risk or low risk health effect. Consuming food after two hours has medium risk. Consuming food after three or four hours has high health risk. If the level of health risk is translated according to syariah law, RTE food which contaminated with more than 2 drops of human stools or contaminated with small amount (1-2 drops) of human stools and left over at ambient temperature more than 4 hours is considered as syubhah/makhrooh (doubtful) to be eaten. 2015 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/63199/1/63199_MICROBIOLOGICAL%20GUIDELINES-abstract.pdf Abu Bakar, Ibrahim and Abdul Rafa, Ainin Azwani and Abdullah Sani, Norrakiah (2015) Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food. In: ASEAN Food Conference 2015, 24th-26th June 2015, Pasay City, Manila Philippines. (Unpublished) https://www.facebook.com/AFC2015/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology TP368 Food processing and manufacture
spellingShingle	QR Microbiology TP368 Food processing and manufacture Abu Bakar, Ibrahim Abdul Rafa, Ainin Azwani Abdullah Sani, Norrakiah Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food
description	This study was conducted to evaluate the severity of najs (filth) contamination in ready to eat food as to confirm the definition of halal food which suppose not contaminated with najs mutawwassitah (medium filth). A total of 52 human stools samples were collected and the screening of bacteria in the human stools samples were done according to traditional microbiological analysis methods. Determinations of bacterial growth curves were performed using NanoDrop 1000 UV-VIS Spectrophotometer at 630nm where the initial and end of log times for each of bacteria was determined. The growth evaluation of fecal borne bacteria in ready to eat food was done using prepared fried rice samples which were prepared at food preparation laboratory. The results showed that healthy human stools which fall under najs mutawassitah contain high amount of presumptive pathogenic bacteria specifically E. coli, S. aureus, B. cereus, Aromonas spp. and Salmonella spp. at different mean values. Average lag phase time for faecal borne bacteria is about 60 minutes (one hour). Thus consuming food before one hour should not give any significant health effect. Consuming food which contains faecal borne bacteria within one to two hours will give either no risk or low risk health effect. Consuming food after two hours has medium risk. Consuming food after three or four hours has high health risk. If the level of health risk is translated according to syariah law, RTE food which contaminated with more than 2 drops of human stools or contaminated with small amount (1-2 drops) of human stools and left over at ambient temperature more than 4 hours is considered as syubhah/makhrooh (doubtful) to be eaten.
format	Conference or Workshop Item
author	Abu Bakar, Ibrahim Abdul Rafa, Ainin Azwani Abdullah Sani, Norrakiah
author_facet	Abu Bakar, Ibrahim Abdul Rafa, Ainin Azwani Abdullah Sani, Norrakiah
author_sort	Abu Bakar, Ibrahim
title	Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food
title_short	Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food
title_full	Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food
title_fullStr	Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food
title_full_unstemmed	Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food
title_sort	microbiological guidelines of medium najs (filth) contamination in halal ready to eat food
publishDate	2015
url	http://irep.iium.edu.my/63199/ http://irep.iium.edu.my/63199/ http://irep.iium.edu.my/63199/1/63199_MICROBIOLOGICAL%20GUIDELINES-abstract.pdf
first_indexed	2023-09-18T21:29:39Z
last_indexed	2023-09-18T21:29:39Z
_version_	1777412424790441984

Microbiological guidelines of medium najs (filth) contamination in Halal ready to eat food

Similar Items