High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods
The urge of understanding soil microbial function and diversity of peatland soils either forested or non-forested has emerged since over a decade ago concerning the ongoing the ecosystem deterioration and its environmental impacts it causes. Like any other soil environments, this unique ecosystem po...
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iium-619292018-02-14T08:29:54Z http://irep.iium.edu.my/61929/ High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods Mohamad Roslan, Muhamad Aidilfitri Mohamad, Mohd Azrul Naim Mohd Omar, Suhaila QR Microbiology The urge of understanding soil microbial function and diversity of peatland soils either forested or non-forested has emerged since over a decade ago concerning the ongoing the ecosystem deterioration and its environmental impacts it causes. Like any other soil environments, this unique ecosystem possesses great challenges in isolating quality DNA prerequisite for microbial ecology studies in virtue of its physicochemical characteristics. Two general approaches have been extensively applied for soil microbial DNA extraction which are termed as direct DNA extraction protocol and indirect DNA extraction protocol. The only step differentiate between these two protocols is the later includes additional cell separation method from soil microaggregates preceding DNA extraction process. Therefore, several improved and modified methods in conventional DNA extraction and purification methods are reviewed in this paper to cater all the highlighted issues as to obtain high-quality DNA for soil peatland metagenomics studies. RAZI Publishing 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61929/1/HIGH-QUALITY%20DNA%20FROM%20PEAT%20SOIL%20FOR%20METAGENOMIC%20STUDIES%20%20.pdf Mohamad Roslan, Muhamad Aidilfitri and Mohamad, Mohd Azrul Naim and Mohd Omar, Suhaila (2017) High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods. Science Heritage Journal. pp. 1-6. ISSN 2521-0858 E-ISSN 2521-0866 https://zibelinepub.com/archives/2gws2017/2gws2017-01-06.pdf |
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QR Microbiology Mohamad Roslan, Muhamad Aidilfitri Mohamad, Mohd Azrul Naim Mohd Omar, Suhaila High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods |
description |
The urge of understanding soil microbial function and diversity of peatland soils either forested or non-forested has emerged since over a decade ago concerning the ongoing the ecosystem deterioration and its environmental impacts it causes. Like any other soil environments, this unique ecosystem possesses great challenges in isolating quality DNA prerequisite for microbial ecology studies in virtue of its physicochemical characteristics. Two general approaches have been extensively applied for soil microbial DNA extraction which are termed as direct DNA extraction protocol and indirect DNA extraction protocol. The only step differentiate between these two protocols is the later includes additional cell separation method from soil microaggregates preceding DNA extraction process. Therefore, several improved and modified methods in conventional DNA extraction and purification methods are reviewed in this paper to cater all the highlighted issues as to obtain high-quality DNA for soil peatland metagenomics studies. |
format |
Article |
author |
Mohamad Roslan, Muhamad Aidilfitri Mohamad, Mohd Azrul Naim Mohd Omar, Suhaila |
author_facet |
Mohamad Roslan, Muhamad Aidilfitri Mohamad, Mohd Azrul Naim Mohd Omar, Suhaila |
author_sort |
Mohamad Roslan, Muhamad Aidilfitri |
title |
High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods |
title_short |
High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods |
title_full |
High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods |
title_fullStr |
High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods |
title_full_unstemmed |
High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods |
title_sort |
high quality dna from peat soil for metagenomic studies: a minireview on dna extraction methods |
publisher |
RAZI Publishing |
publishDate |
2017 |
url |
http://irep.iium.edu.my/61929/ http://irep.iium.edu.my/61929/ http://irep.iium.edu.my/61929/1/HIGH-QUALITY%20DNA%20FROM%20PEAT%20SOIL%20FOR%20METAGENOMIC%20STUDIES%20%20.pdf |
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2023-09-18T21:27:51Z |
last_indexed |
2023-09-18T21:27:51Z |
_version_ |
1777412311725637632 |