High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods

The urge of understanding soil microbial function and diversity of peatland soils either forested or non-forested has emerged since over a decade ago concerning the ongoing the ecosystem deterioration and its environmental impacts it causes. Like any other soil environments, this unique ecosystem po...

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Main Authors: Mohamad Roslan, Muhamad Aidilfitri, Mohamad, Mohd Azrul Naim, Mohd Omar, Suhaila
Format: Article
Language:English
Published: RAZI Publishing 2017
Subjects:
Online Access:http://irep.iium.edu.my/61929/
http://irep.iium.edu.my/61929/
http://irep.iium.edu.my/61929/1/HIGH-QUALITY%20DNA%20FROM%20PEAT%20SOIL%20FOR%20METAGENOMIC%20STUDIES%20%20.pdf
id iium-61929
recordtype eprints
spelling iium-619292018-02-14T08:29:54Z http://irep.iium.edu.my/61929/ High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods Mohamad Roslan, Muhamad Aidilfitri Mohamad, Mohd Azrul Naim Mohd Omar, Suhaila QR Microbiology The urge of understanding soil microbial function and diversity of peatland soils either forested or non-forested has emerged since over a decade ago concerning the ongoing the ecosystem deterioration and its environmental impacts it causes. Like any other soil environments, this unique ecosystem possesses great challenges in isolating quality DNA prerequisite for microbial ecology studies in virtue of its physicochemical characteristics. Two general approaches have been extensively applied for soil microbial DNA extraction which are termed as direct DNA extraction protocol and indirect DNA extraction protocol. The only step differentiate between these two protocols is the later includes additional cell separation method from soil microaggregates preceding DNA extraction process. Therefore, several improved and modified methods in conventional DNA extraction and purification methods are reviewed in this paper to cater all the highlighted issues as to obtain high-quality DNA for soil peatland metagenomics studies. RAZI Publishing 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61929/1/HIGH-QUALITY%20DNA%20FROM%20PEAT%20SOIL%20FOR%20METAGENOMIC%20STUDIES%20%20.pdf Mohamad Roslan, Muhamad Aidilfitri and Mohamad, Mohd Azrul Naim and Mohd Omar, Suhaila (2017) High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods. Science Heritage Journal. pp. 1-6. ISSN 2521-0858 E-ISSN 2521-0866 https://zibelinepub.com/archives/2gws2017/2gws2017-01-06.pdf
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QR Microbiology
spellingShingle QR Microbiology
Mohamad Roslan, Muhamad Aidilfitri
Mohamad, Mohd Azrul Naim
Mohd Omar, Suhaila
High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods
description The urge of understanding soil microbial function and diversity of peatland soils either forested or non-forested has emerged since over a decade ago concerning the ongoing the ecosystem deterioration and its environmental impacts it causes. Like any other soil environments, this unique ecosystem possesses great challenges in isolating quality DNA prerequisite for microbial ecology studies in virtue of its physicochemical characteristics. Two general approaches have been extensively applied for soil microbial DNA extraction which are termed as direct DNA extraction protocol and indirect DNA extraction protocol. The only step differentiate between these two protocols is the later includes additional cell separation method from soil microaggregates preceding DNA extraction process. Therefore, several improved and modified methods in conventional DNA extraction and purification methods are reviewed in this paper to cater all the highlighted issues as to obtain high-quality DNA for soil peatland metagenomics studies.
format Article
author Mohamad Roslan, Muhamad Aidilfitri
Mohamad, Mohd Azrul Naim
Mohd Omar, Suhaila
author_facet Mohamad Roslan, Muhamad Aidilfitri
Mohamad, Mohd Azrul Naim
Mohd Omar, Suhaila
author_sort Mohamad Roslan, Muhamad Aidilfitri
title High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods
title_short High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods
title_full High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods
title_fullStr High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods
title_full_unstemmed High quality DNA from peat soil for metagenomic studies: A minireview on DNA extraction methods
title_sort high quality dna from peat soil for metagenomic studies: a minireview on dna extraction methods
publisher RAZI Publishing
publishDate 2017
url http://irep.iium.edu.my/61929/
http://irep.iium.edu.my/61929/
http://irep.iium.edu.my/61929/1/HIGH-QUALITY%20DNA%20FROM%20PEAT%20SOIL%20FOR%20METAGENOMIC%20STUDIES%20%20.pdf
first_indexed 2023-09-18T21:27:51Z
last_indexed 2023-09-18T21:27:51Z
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