A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis
PRDM9 binding localizes almost all meiotic recombination sites in humans and mice. However, most PRDM9-bound loci do not become recombination hotspots. To explore factors that affect binding and subsequent recombination outcomes, we mapped human PRDM9 binding sites in a transfected human cell line a...
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| Language: | English English |
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eLife Sciences Publications
2017
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| Online Access: | http://irep.iium.edu.my/59029/ http://irep.iium.edu.my/59029/ http://irep.iium.edu.my/59029/ http://irep.iium.edu.my/59029/13/59029-A%20map%20of%20human%20PRDM9%20binding.pdf http://irep.iium.edu.my/59029/7/59029-A%20map%20of%20human%20PRDM9%20binding_scopus.pdf |
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iium-590292018-03-28T01:02:31Z http://irep.iium.edu.my/59029/ A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis Altemose, Nicolas Noor, Nudrat Bitoun, Emmanuelle Tumian, Afidalina Imbeault, Michaël Chapman, J. Ross Aricescu, A. Radu Myers, Simon R. QH301 Biology QH426 Genetics PRDM9 binding localizes almost all meiotic recombination sites in humans and mice. However, most PRDM9-bound loci do not become recombination hotspots. To explore factors that affect binding and subsequent recombination outcomes, we mapped human PRDM9 binding sites in a transfected human cell line and measured PRDM9-induced histone modifications. These data reveal varied DNA-binding modalities of PRDM9. We also find that human PRDM9 frequently binds promoters, despite their low recombination rates, and it can activate expression of a small number of genes including CTCFL and VCX. Furthermore, we identify specific sequence motifs that predict consistent, localized meiotic recombination suppression around a subset of PRDM9 binding sites. These motifs strongly associate with KRAB-ZNF protein binding, TRIM28 recruitment, and specific histone modifications. Finally, we demonstrate that, in addition to binding DNA, PRDM9's zinc fingers also mediate its multimerization, and we show that a pair of highly diverged alleles preferentially form homo-multimers. eLife Sciences Publications 2017-10-26 Article PeerReviewed application/pdf en http://irep.iium.edu.my/59029/13/59029-A%20map%20of%20human%20PRDM9%20binding.pdf application/pdf en http://irep.iium.edu.my/59029/7/59029-A%20map%20of%20human%20PRDM9%20binding_scopus.pdf Altemose, Nicolas and Noor, Nudrat and Bitoun, Emmanuelle and Tumian, Afidalina and Imbeault, Michaël and Chapman, J. Ross and Aricescu, A. Radu and Myers, Simon R. (2017) A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis. eLife, 6. pp. 1-46. E-ISSN 2050-084X https://elifesciences.org/articles/28383 10.7554/eLife.28383 |
| repository_type |
Digital Repository |
| institution_category |
Local University |
| institution |
International Islamic University Malaysia |
| building |
IIUM Repository |
| collection |
Online Access |
| language |
English English |
| topic |
QH301 Biology QH426 Genetics |
| spellingShingle |
QH301 Biology QH426 Genetics Altemose, Nicolas Noor, Nudrat Bitoun, Emmanuelle Tumian, Afidalina Imbeault, Michaël Chapman, J. Ross Aricescu, A. Radu Myers, Simon R. A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis |
| description |
PRDM9 binding localizes almost all meiotic recombination sites in humans and mice. However, most PRDM9-bound loci do not become recombination hotspots. To explore factors that affect binding and subsequent recombination outcomes, we mapped human PRDM9 binding sites in a transfected human cell line and measured PRDM9-induced histone modifications. These data reveal varied DNA-binding modalities of PRDM9. We also find that human PRDM9 frequently binds promoters, despite their low recombination rates, and it can activate expression of a small number of genes including CTCFL and VCX. Furthermore, we identify specific sequence motifs that predict consistent, localized meiotic recombination suppression around a subset of PRDM9 binding sites. These motifs strongly associate with KRAB-ZNF protein binding, TRIM28 recruitment, and specific histone modifications. Finally, we demonstrate that, in addition to binding DNA, PRDM9's zinc fingers also mediate its multimerization, and we show that a pair of highly diverged alleles preferentially form homo-multimers. |
| format |
Article |
| author |
Altemose, Nicolas Noor, Nudrat Bitoun, Emmanuelle Tumian, Afidalina Imbeault, Michaël Chapman, J. Ross Aricescu, A. Radu Myers, Simon R. |
| author_facet |
Altemose, Nicolas Noor, Nudrat Bitoun, Emmanuelle Tumian, Afidalina Imbeault, Michaël Chapman, J. Ross Aricescu, A. Radu Myers, Simon R. |
| author_sort |
Altemose, Nicolas |
| title |
A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis |
| title_short |
A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis |
| title_full |
A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis |
| title_fullStr |
A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis |
| title_full_unstemmed |
A map of human PRDM9 binding provides evidence for novel behaviors of PRDM9 and other zinc-finger proteins in meiosis |
| title_sort |
map of human prdm9 binding provides evidence for novel behaviors of prdm9 and other zinc-finger proteins in meiosis |
| publisher |
eLife Sciences Publications |
| publishDate |
2017 |
| url |
http://irep.iium.edu.my/59029/ http://irep.iium.edu.my/59029/ http://irep.iium.edu.my/59029/ http://irep.iium.edu.my/59029/13/59029-A%20map%20of%20human%20PRDM9%20binding.pdf http://irep.iium.edu.my/59029/7/59029-A%20map%20of%20human%20PRDM9%20binding_scopus.pdf |
| first_indexed |
2023-09-18T21:23:34Z |
| last_indexed |
2023-09-18T21:23:34Z |
| _version_ |
1777412041998336000 |