Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters
E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures...
Main Authors: | , |
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Format: | Article |
Language: | English English |
Published: |
Open science Publisher
2017
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Subjects: | |
Online Access: | http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/1/Japs_Tg_Affaro.pdf http://irep.iium.edu.my/57890/7/57890_Investigation%20on%20plasmid%20DNA%20separation_SCOPUS.pdf |