Association of MMP-9 gene polymorphisms with nephrolithiasis patients

Background: Nephrolithiasis is one of the causes which lead to chronic kidney disease (CKD). Matrix metalloproteinases (MMPs) are endopeptidases degrading extracellular matrix which correlate with the pathogenesis of atherosclerosis. The current study was designed to analyze the association of (R...

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Main Authors:	Mehde, Atheer Awad, Mehdi, Wesen Adel, Yusof, Faridah, Raus, Raha Bt. Ahmad, Zainal Abidin, Zaima Azira, Ghazali, Hamid, Abd Rahman, Azlina
Format:	Article
Language:	English English English
Published:	Wiley-Liss Inc. 2017
Subjects:	R Medicine (General)
Online Access:	http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/8/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with_scopus.pdf http://irep.iium.edu.my/55862/15/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with%20nephrolithiasis%20patients_WoS.pdf http://irep.iium.edu.my/55862/20/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with%20nephrolithiasis%20patients.pdf

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spelling	iium-558622018-07-31T00:48:35Z http://irep.iium.edu.my/55862/ Association of MMP-9 gene polymorphisms with nephrolithiasis patients Mehde, Atheer Awad Mehdi, Wesen Adel Yusof, Faridah Raus, Raha Bt. Ahmad Zainal Abidin, Zaima Azira Ghazali, Hamid Abd Rahman, Azlina R Medicine (General) Background: Nephrolithiasis is one of the causes which lead to chronic kidney disease (CKD). Matrix metalloproteinases (MMPs) are endopeptidases degrading extracellular matrix which correlate with the pathogenesis of atherosclerosis. The current study was designed to analyze the association of (R279Q, C1562T) polymorphism of MMP-9 with nephrolithiasis patients. Methods: Genotyping of MMP-9/R279Q and of MMP-9/C1562T polymorphism were carried out by PCR-based restriction digestion method. Serum level of MMP-9, oxidative stress marker, MDA, and uric acid were measured in patients and control. Results: Allele frequencies of the MMP-9/C1562T polymorphism for C and T allele were 71.25% and 28.75% in patients, 87.08% and 12.92% in control respectively. The homozygote TT was more frequent in the nephrolithiasis patients group, while T allele frequency was significantly higher in the nephrolithiasis patients group than in the control group. The patients with CT and TT genotype showed a significant increase in serum MMP-9, Total Oxidant Status (TOS), Oxidative Stress Index (OSI), Malondialdehyde (MDA), and uric acid when compared to CC genotype in patients with nephrolithiasis. The R279Q polymorphism site with regard to the relationship with nephrolithiasis was not significant. Conclusion: The result indicates that patients with TT genotype had an increased risk of stones. Also, the results demonstrate that TT allele of the C1562T polymorphism in the MMP-9gene is related with an increase of oxidative stress in nephrolithiasis patients and may possibly impose a risk for cardiovascular diseases in patients with TT genotype of MMP-9. Wiley-Liss Inc. 2017 Article PeerReviewed application/pdf en http://irep.iium.edu.my/55862/8/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with_scopus.pdf application/pdf en http://irep.iium.edu.my/55862/15/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with%20nephrolithiasis%20patients_WoS.pdf application/pdf en http://irep.iium.edu.my/55862/20/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with%20nephrolithiasis%20patients.pdf Mehde, Atheer Awad and Mehdi, Wesen Adel and Yusof, Faridah and Raus, Raha Bt. Ahmad and Zainal Abidin, Zaima Azira and Ghazali, Hamid and Abd Rahman, Azlina (2017) Association of MMP-9 gene polymorphisms with nephrolithiasis patients. Journal of Clinical Laboratory Analysis, 32 (1). pp. 1-6. ISSN 0887-8013 E-ISSN 1098-2825 http://onlinelibrary.wiley.com/doi/10.1002/jcla.22173/abstract 10.1002/jcla.22173
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Mehde, Atheer Awad Mehdi, Wesen Adel Yusof, Faridah Raus, Raha Bt. Ahmad Zainal Abidin, Zaima Azira Ghazali, Hamid Abd Rahman, Azlina Association of MMP-9 gene polymorphisms with nephrolithiasis patients
description	Background: Nephrolithiasis is one of the causes which lead to chronic kidney disease (CKD). Matrix metalloproteinases (MMPs) are endopeptidases degrading extracellular matrix which correlate with the pathogenesis of atherosclerosis. The current study was designed to analyze the association of (R279Q, C1562T) polymorphism of MMP-9 with nephrolithiasis patients. Methods: Genotyping of MMP-9/R279Q and of MMP-9/C1562T polymorphism were carried out by PCR-based restriction digestion method. Serum level of MMP-9, oxidative stress marker, MDA, and uric acid were measured in patients and control. Results: Allele frequencies of the MMP-9/C1562T polymorphism for C and T allele were 71.25% and 28.75% in patients, 87.08% and 12.92% in control respectively. The homozygote TT was more frequent in the nephrolithiasis patients group, while T allele frequency was significantly higher in the nephrolithiasis patients group than in the control group. The patients with CT and TT genotype showed a significant increase in serum MMP-9, Total Oxidant Status (TOS), Oxidative Stress Index (OSI), Malondialdehyde (MDA), and uric acid when compared to CC genotype in patients with nephrolithiasis. The R279Q polymorphism site with regard to the relationship with nephrolithiasis was not significant. Conclusion: The result indicates that patients with TT genotype had an increased risk of stones. Also, the results demonstrate that TT allele of the C1562T polymorphism in the MMP-9gene is related with an increase of oxidative stress in nephrolithiasis patients and may possibly impose a risk for cardiovascular diseases in patients with TT genotype of MMP-9.
format	Article
author	Mehde, Atheer Awad Mehdi, Wesen Adel Yusof, Faridah Raus, Raha Bt. Ahmad Zainal Abidin, Zaima Azira Ghazali, Hamid Abd Rahman, Azlina
author_facet	Mehde, Atheer Awad Mehdi, Wesen Adel Yusof, Faridah Raus, Raha Bt. Ahmad Zainal Abidin, Zaima Azira Ghazali, Hamid Abd Rahman, Azlina
author_sort	Mehde, Atheer Awad
title	Association of MMP-9 gene polymorphisms with nephrolithiasis patients
title_short	Association of MMP-9 gene polymorphisms with nephrolithiasis patients
title_full	Association of MMP-9 gene polymorphisms with nephrolithiasis patients
title_fullStr	Association of MMP-9 gene polymorphisms with nephrolithiasis patients
title_full_unstemmed	Association of MMP-9 gene polymorphisms with nephrolithiasis patients
title_sort	association of mmp-9 gene polymorphisms with nephrolithiasis patients
publisher	Wiley-Liss Inc.
publishDate	2017
url	http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/ http://irep.iium.edu.my/55862/8/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with_scopus.pdf http://irep.iium.edu.my/55862/15/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with%20nephrolithiasis%20patients_WoS.pdf http://irep.iium.edu.my/55862/20/55862_Association%20of%20MMP-9%20gene%20polymorphisms%20with%20nephrolithiasis%20patients.pdf
first_indexed	2023-09-18T21:18:49Z
last_indexed	2023-09-18T21:18:49Z
_version_	1777411743913345024

Association of MMP-9 gene polymorphisms with nephrolithiasis patients

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