Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody

Competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of porcine gelatin in edible bird's nest (EBN). Three ELISAs were developed by using polyclonal rabbit antibodies against porcine species-specific amino acid sequences of collagen a2 (I) chain (pAb1...

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Main Authors:	Tukiran, Nur Azira, Ismail, Amin, Mustafa, Shuhaimi, Hamid, Muhajir
Format:	Article
Language:	English English English
Published:	Elsevier Ltd 2016
Subjects:	TP Chemical technology
Online Access:	http://irep.iium.edu.my/54627/ http://irep.iium.edu.my/54627/ http://irep.iium.edu.my/54627/ http://irep.iium.edu.my/54627/1/Food%20Control%202016%2059%20561%20-%20566.pdf http://irep.iium.edu.my/54627/7/54627_Determination%20of%20porcine%20gelatin_WOS.pdf http://irep.iium.edu.my/54627/8/54627_Determination%20of%20porcine%20gelatin_SCOPUS.pdf

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recordtype	eprints
spelling	iium-546272017-07-18T00:41:19Z http://irep.iium.edu.my/54627/ Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir TP Chemical technology Competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of porcine gelatin in edible bird's nest (EBN). Three ELISAs were developed by using polyclonal rabbit antibodies against porcine species-specific amino acid sequences of collagen a2 (I) chain (pAb1 and pAb2) and a1 (I) chain (pAb3). The limit of detection (IC15) of the three ELISAs was 0.033, 0.082 and 0.052 mg/mL respectively. The median inhibitory concentration (IC50) of pAb1, pAb2 and pAb3 was 0.265, 0.394 and 0.228 mg/mL respectively, as well as able to recognise porcine and bovine gelatins. pAb1 showed slight cross-reactivity with cave nest and egg white, while pAb2 exhibited slight cross-reactivity with blood cave nest and egg white. No cross-reactivity was observed with EBNs and egg white for pAb3. The recoveries of porcine gelatin spiked EBNs were in the range of 62.8e125.4% with intra- and inter-day coefficient of variants (CVs) of 2.9e5.4% and 4.7e9.6% respectively when using pAb3. Taking into account all abovementioned factors, pAb3 appeared sufficient for EBN authentication. Elsevier Ltd 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54627/1/Food%20Control%202016%2059%20561%20-%20566.pdf application/pdf en http://irep.iium.edu.my/54627/7/54627_Determination%20of%20porcine%20gelatin_WOS.pdf application/pdf en http://irep.iium.edu.my/54627/8/54627_Determination%20of%20porcine%20gelatin_SCOPUS.pdf Tukiran, Nur Azira and Ismail, Amin and Mustafa, Shuhaimi and Hamid, Muhajir (2016) Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody. Food Control, 59. pp. 561-566. ISSN 0956-7135 E-ISSN 1873-7129 http://www.sciencedirect.com/science/article/pii/S0956713515300682 10.1016/j.foodcont.2015.06.039
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody
description	Competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of porcine gelatin in edible bird's nest (EBN). Three ELISAs were developed by using polyclonal rabbit antibodies against porcine species-specific amino acid sequences of collagen a2 (I) chain (pAb1 and pAb2) and a1 (I) chain (pAb3). The limit of detection (IC15) of the three ELISAs was 0.033, 0.082 and 0.052 mg/mL respectively. The median inhibitory concentration (IC50) of pAb1, pAb2 and pAb3 was 0.265, 0.394 and 0.228 mg/mL respectively, as well as able to recognise porcine and bovine gelatins. pAb1 showed slight cross-reactivity with cave nest and egg white, while pAb2 exhibited slight cross-reactivity with blood cave nest and egg white. No cross-reactivity was observed with EBNs and egg white for pAb3. The recoveries of porcine gelatin spiked EBNs were in the range of 62.8e125.4% with intra- and inter-day coefficient of variants (CVs) of 2.9e5.4% and 4.7e9.6% respectively when using pAb3. Taking into account all abovementioned factors, pAb3 appeared sufficient for EBN authentication.
format	Article
author	Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir
author_facet	Tukiran, Nur Azira Ismail, Amin Mustafa, Shuhaimi Hamid, Muhajir
author_sort	Tukiran, Nur Azira
title	Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody
title_short	Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody
title_full	Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody
title_fullStr	Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody
title_full_unstemmed	Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody
title_sort	determination of porcine gelatin in edible bird's nest by competitive indirect elisa based on anti-peptide polyclonal antibody
publisher	Elsevier Ltd
publishDate	2016
url	http://irep.iium.edu.my/54627/ http://irep.iium.edu.my/54627/ http://irep.iium.edu.my/54627/ http://irep.iium.edu.my/54627/1/Food%20Control%202016%2059%20561%20-%20566.pdf http://irep.iium.edu.my/54627/7/54627_Determination%20of%20porcine%20gelatin_WOS.pdf http://irep.iium.edu.my/54627/8/54627_Determination%20of%20porcine%20gelatin_SCOPUS.pdf
first_indexed	2023-09-18T21:17:16Z
last_indexed	2023-09-18T21:17:16Z
_version_	1777411646197596160

Determination of porcine gelatin in edible bird's nest by competitive indirect ELISA based on anti-peptide polyclonal antibody

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