Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma

DNA methylation silences the gene through addition of methyl group. p16, a tumor suppressor gene that inhibits cyclin-dependent kinase, inactivates the Rb protein and blocks G1 phase in a normal cell cycle. A DNA repair gene, MGMT removes alkyl adduct to a cysteine residue within the protein...

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Main Authors:	Mohd. Ridah, Lailatul Jalilah, Zainuddin, Norafiza, A. Talib, Norlelawati, Hussain, Faezahtul Arbaeyah
Format:	Conference or Workshop Item
Language:	English
Published:	2015
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/54383/ http://irep.iium.edu.my/54383/1/ICBHSR.pdf

id	iium-54383
recordtype	eprints
spelling	iium-543832017-01-20T03:48:31Z http://irep.iium.edu.my/54383/ Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma Mohd. Ridah, Lailatul Jalilah Zainuddin, Norafiza A. Talib, Norlelawati Hussain, Faezahtul Arbaeyah RC0254 Neoplasms. Tumors. Oncology (including Cancer) DNA methylation silences the gene through addition of methyl group. p16, a tumor suppressor gene that inhibits cyclin-dependent kinase, inactivates the Rb protein and blocks G1 phase in a normal cell cycle. A DNA repair gene, MGMT removes alkyl adduct to a cysteine residue within the protein, thus preventing lethal cross-links. p16 and MGMT methylation has been reported to associate with DLBCL. A member of the extracellular chondroitin and heparin sulfate proteoglycans, SPOCK2 functions mainly in extracellular matrix for cell adhesion. Uniquely, SPOCK2 (testican 2) abolishes the inhibition of membrane-type 1-matrix metalloproteinase by other testican family which might enhance the angiogenesis. In this study, we aimed to screen for aberrantly methylated genes which might contribute to the pathogenesis of DLBCL using methylation specific PCR (MSP). p16 methylation was identified in 64 (73%) of 88 samples. On the other hand, SPOCK2 was found to be unmethylated in 30 (34%) samples. Interestingly, MGMT methylation was detected in all cases. We also found an association between p16 methylation status with patients aged >50 years old (p= 0.023). This finding is parallel with an animal study showing that aging increases p16 methylation. No association was found between the methylation of other genes with age. Unmethylation of SPOCK2 might cause testican 2 expressions, which has been suggested to contribute toward malignant behaviour. MGMT was reported to be methylated among cancer patients who smoke, drink and are non-vegetarian. Thus, it is hypothesized that lifestyle might affect MGMT methylation in this study population. 2015-01 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54383/1/ICBHSR.pdf Mohd. Ridah, Lailatul Jalilah and Zainuddin, Norafiza and A. Talib, Norlelawati and Hussain, Faezahtul Arbaeyah (2015) Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma. In: International Conference on Biomedical and Health Sciences Research 2015, 25-27 Jan 2015, Putrajaya. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Mohd. Ridah, Lailatul Jalilah Zainuddin, Norafiza A. Talib, Norlelawati Hussain, Faezahtul Arbaeyah Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma
description	DNA methylation silences the gene through addition of methyl group. p16, a tumor suppressor gene that inhibits cyclin-dependent kinase, inactivates the Rb protein and blocks G1 phase in a normal cell cycle. A DNA repair gene, MGMT removes alkyl adduct to a cysteine residue within the protein, thus preventing lethal cross-links. p16 and MGMT methylation has been reported to associate with DLBCL. A member of the extracellular chondroitin and heparin sulfate proteoglycans, SPOCK2 functions mainly in extracellular matrix for cell adhesion. Uniquely, SPOCK2 (testican 2) abolishes the inhibition of membrane-type 1-matrix metalloproteinase by other testican family which might enhance the angiogenesis. In this study, we aimed to screen for aberrantly methylated genes which might contribute to the pathogenesis of DLBCL using methylation specific PCR (MSP). p16 methylation was identified in 64 (73%) of 88 samples. On the other hand, SPOCK2 was found to be unmethylated in 30 (34%) samples. Interestingly, MGMT methylation was detected in all cases. We also found an association between p16 methylation status with patients aged >50 years old (p= 0.023). This finding is parallel with an animal study showing that aging increases p16 methylation. No association was found between the methylation of other genes with age. Unmethylation of SPOCK2 might cause testican 2 expressions, which has been suggested to contribute toward malignant behaviour. MGMT was reported to be methylated among cancer patients who smoke, drink and are non-vegetarian. Thus, it is hypothesized that lifestyle might affect MGMT methylation in this study population.
format	Conference or Workshop Item
author	Mohd. Ridah, Lailatul Jalilah Zainuddin, Norafiza A. Talib, Norlelawati Hussain, Faezahtul Arbaeyah
author_facet	Mohd. Ridah, Lailatul Jalilah Zainuddin, Norafiza A. Talib, Norlelawati Hussain, Faezahtul Arbaeyah
author_sort	Mohd. Ridah, Lailatul Jalilah
title	Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma
title_short	Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma
title_full	Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma
title_fullStr	Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma
title_full_unstemmed	Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma
title_sort	methylation-specific pcr revealed aberrant promoter gene methylation of p16, mgmt and spock2 in diffuse large b-cell lymphoma
publishDate	2015
url	http://irep.iium.edu.my/54383/ http://irep.iium.edu.my/54383/1/ICBHSR.pdf
first_indexed	2023-09-18T21:16:56Z
last_indexed	2023-09-18T21:16:56Z
_version_	1777411625577349120

Methylation-specific PCR Revealed Aberrant Promoter Gene Methylation of p16, MGMT and SPOCK2 in Diffuse Large B-cell Lymphoma

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