Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma

MGMT (O6-Methylguanine-DNA Methyltransferase) suppresses tumour development by removing alkyl adduct, while SPOCK2 (SPARC/Osteonectin CWCV and Kazal-like domains proteoglycan) abolishes the inhibition of membrane-type matrix metalloproteinases (MT-MMP) which leads to angiogenesis. Hence, MGMT methyl...

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Main Authors:	Zainuddin, Norafiza, Mohd. Ridah, Lailatul Jalilah, Omar, Aqilah Nabihah, A. Talib, Norlelawati, Muhammad, Naznin, Hussain, Faezatul Arbaeyah
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Online Access:	http://irep.iium.edu.my/54375/ http://irep.iium.edu.my/54375/ http://irep.iium.edu.my/54375/1/54375_abstract.pdf

id	iium-54375
recordtype	eprints
spelling	iium-543752017-05-15T04:09:45Z http://irep.iium.edu.my/54375/ Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma Zainuddin, Norafiza Mohd. Ridah, Lailatul Jalilah Omar, Aqilah Nabihah A. Talib, Norlelawati Muhammad, Naznin Hussain, Faezatul Arbaeyah RC0254 Neoplasms. Tumors. Oncology (including Cancer) MGMT (O6-Methylguanine-DNA Methyltransferase) suppresses tumour development by removing alkyl adduct, while SPOCK2 (SPARC/Osteonectin CWCV and Kazal-like domains proteoglycan) abolishes the inhibition of membrane-type matrix metalloproteinases (MT-MMP) which leads to angiogenesis. Hence, MGMT methylation may initiate malignant cells transformation. In contrast, SPOCK2 methylation is hypothesized not to be a common event in diffuse large B-cell lymphoma (DLBCL). In this study, we examined the methylation status of MGMT and SPOCK2 in DLBCL as in Malaysia the information is extremely lacking. A total of 88 formalin-fixed paraffin-embedded tissue of patients diagnosed with DLBCL from the year 2006 to 2013 were retrieved from Hospital Universiti Sains Malaysia and Hospital Tengku Ampuan Afzan. Methylation-specific PCR (MSP) was used to examine the methylation status of both genes. Interestingly, methylation of MGMT was detected in all the 88 DLBCL samples, whereas SPOCK2 was found to be methylated in 83 of 88 (94.3%) DLBCL cases. Our study showed a remarkably high percentage of promoter methylation of both MGMT and SPOCK2 genes. Our finding also negates initial expectation that SPOCK2 methylation would be an uncommon event in the majority of DLBCL cases. This study has shown a very high percentage of promoter methylation of MGMT and SPOCK2 in the DLBCL cases studied by MSP, using archival lymphoma tissues. Nonetheless, additional research is needed to quantitatively evaluate MGMT and SPOCK2 methylation, and to analyse gene expression and/or protein expression in order to further understand the role of MGMT and SPOCK2 methylation in the pathogenesis of DLBCL. 2016-12 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/54375/1/54375_abstract.pdf Zainuddin, Norafiza and Mohd. Ridah, Lailatul Jalilah and Omar, Aqilah Nabihah and A. Talib, Norlelawati and Muhammad, Naznin and Hussain, Faezatul Arbaeyah (2016) Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma. In: 3rd Pan Asian Biomedical Science Conference, 7-8 Dec 2016, Kuala Lumpur. (Unpublished) https://sites.google.com/site/3rdpabsc2016/
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RC0254 Neoplasms. Tumors. Oncology (including Cancer)
spellingShingle	RC0254 Neoplasms. Tumors. Oncology (including Cancer) Zainuddin, Norafiza Mohd. Ridah, Lailatul Jalilah Omar, Aqilah Nabihah A. Talib, Norlelawati Muhammad, Naznin Hussain, Faezatul Arbaeyah Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma
description	MGMT (O6-Methylguanine-DNA Methyltransferase) suppresses tumour development by removing alkyl adduct, while SPOCK2 (SPARC/Osteonectin CWCV and Kazal-like domains proteoglycan) abolishes the inhibition of membrane-type matrix metalloproteinases (MT-MMP) which leads to angiogenesis. Hence, MGMT methylation may initiate malignant cells transformation. In contrast, SPOCK2 methylation is hypothesized not to be a common event in diffuse large B-cell lymphoma (DLBCL). In this study, we examined the methylation status of MGMT and SPOCK2 in DLBCL as in Malaysia the information is extremely lacking. A total of 88 formalin-fixed paraffin-embedded tissue of patients diagnosed with DLBCL from the year 2006 to 2013 were retrieved from Hospital Universiti Sains Malaysia and Hospital Tengku Ampuan Afzan. Methylation-specific PCR (MSP) was used to examine the methylation status of both genes. Interestingly, methylation of MGMT was detected in all the 88 DLBCL samples, whereas SPOCK2 was found to be methylated in 83 of 88 (94.3%) DLBCL cases. Our study showed a remarkably high percentage of promoter methylation of both MGMT and SPOCK2 genes. Our finding also negates initial expectation that SPOCK2 methylation would be an uncommon event in the majority of DLBCL cases. This study has shown a very high percentage of promoter methylation of MGMT and SPOCK2 in the DLBCL cases studied by MSP, using archival lymphoma tissues. Nonetheless, additional research is needed to quantitatively evaluate MGMT and SPOCK2 methylation, and to analyse gene expression and/or protein expression in order to further understand the role of MGMT and SPOCK2 methylation in the pathogenesis of DLBCL.
format	Conference or Workshop Item
author	Zainuddin, Norafiza Mohd. Ridah, Lailatul Jalilah Omar, Aqilah Nabihah A. Talib, Norlelawati Muhammad, Naznin Hussain, Faezatul Arbaeyah
author_facet	Zainuddin, Norafiza Mohd. Ridah, Lailatul Jalilah Omar, Aqilah Nabihah A. Talib, Norlelawati Muhammad, Naznin Hussain, Faezatul Arbaeyah
author_sort	Zainuddin, Norafiza
title	Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma
title_short	Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma
title_full	Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma
title_fullStr	Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma
title_full_unstemmed	Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma
title_sort	methylation status of mgmt and spock2 in diffuse large b-cell lymphoma
publishDate	2016
url	http://irep.iium.edu.my/54375/ http://irep.iium.edu.my/54375/ http://irep.iium.edu.my/54375/1/54375_abstract.pdf
first_indexed	2023-09-18T21:16:55Z
last_indexed	2023-09-18T21:16:55Z
_version_	1777411624543453184

Methylation status of MGMT and SPOCK2 in diffuse large B-cell lymphoma

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