Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting

Phaleria macrocarpa, known as “Mahkota Dewa”, is a widely used medicinal plant in Malaysia. This study focused on the characterization of α-glucosidase inhibitory activity of P. macrocarpa extracts using Fourier transform infrared spectroscopy (FTIR)-based metabolomics. P. macrocarpa and its extract...

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Main Authors:	Easmin, Sabina, Sarker, Md. Zaidul Islam, Ghafoor, Kashif, Ferdosh, Sahena, Md. Jaffri, Juliana, Ali, Md. Eaqub, Mirhosseini, Hamed, Al-Juhaimi, Fahad Y., Perumal, Vikneswari, Khatib, Alfi
Format:	Article
Language:	English English English
Published:	Elsevier 2017
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/53769/ http://irep.iium.edu.my/53769/ http://irep.iium.edu.my/53769/ http://irep.iium.edu.my/53769/12/53769-Rapid%20investigation.pdf http://irep.iium.edu.my/53769/13/53769-Rapid%20investigation_SCOPUS.pdf http://irep.iium.edu.my/53769/24/53769_Rapid%20investigation%20of%20%CE%B1-glucosidase_WOS.pdf

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spelling	iium-537692019-03-05T03:40:01Z http://irep.iium.edu.my/53769/ Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting Easmin, Sabina Sarker, Md. Zaidul Islam Ghafoor, Kashif Ferdosh, Sahena Md. Jaffri, Juliana Ali, Md. Eaqub Mirhosseini, Hamed Al-Juhaimi, Fahad Y. Perumal, Vikneswari Khatib, Alfi RS Pharmacy and materia medica Phaleria macrocarpa, known as “Mahkota Dewa”, is a widely used medicinal plant in Malaysia. This study focused on the characterization of α-glucosidase inhibitory activity of P. macrocarpa extracts using Fourier transform infrared spectroscopy (FTIR)-based metabolomics. P. macrocarpa and its extracts contain thousands of compounds having synergistic effect. Generally, their variability exists, and there are many active components in meager amounts. Thus, the conventional measurement methods of a single component for the quality control are time consuming, laborious, expensive, and unreliable. It is of great interest to develop a rapid prediction method for herbal quality control to investigate the α-glucosidase inhibitory activity of P. macrocarpa by multicomponent analyses. In this study, a rapid and simple analytical method was developed using FTIR spectroscopy-based fingerprinting. A total of 36 extracts of different ethanol concentrations were prepared and tested on inhibitory potential and fingerprinted using FTIR spectroscopy, coupled with chemometrics of orthogonal partial least square (OPLS) at the 4000–400 cm−1 frequency region and resolution of 4 cm−1. The OPLS model generated the highest regression coefficient with R2Y = 0.98 and Q2Y = 0.70, lowest root mean square error estimation = 17.17, and root mean square error of cross validation = 57.29. A five-component (1+4+0) predictive model was build up to correlate FTIR spectra with activity, and the responsible functional groups, such as –CH, –NH, –COOH, and –OH, were identified for the bioactivity. A successful multivariate model was constructed using FTIR-attenuated total reflection as a simple and rapid technique to predict the inhibitory activity. Elsevier 2017-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/53769/12/53769-Rapid%20investigation.pdf application/pdf en http://irep.iium.edu.my/53769/13/53769-Rapid%20investigation_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/53769/24/53769_Rapid%20investigation%20of%20%CE%B1-glucosidase_WOS.pdf Easmin, Sabina and Sarker, Md. Zaidul Islam and Ghafoor, Kashif and Ferdosh, Sahena and Md. Jaffri, Juliana and Ali, Md. Eaqub and Mirhosseini, Hamed and Al-Juhaimi, Fahad Y. and Perumal, Vikneswari and Khatib, Alfi (2017) Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting. Journal of Food and Drug analysis, 25 (2). pp. 306-315. ISSN 1021-9498 E-ISSN 1551-7616 https://ac.els-cdn.com/S1021949816301466/1-s2.0-S1021949816301466-main.pdf?_tid=5ec02b60-af17-11e7-a530-00000aacb361&acdnat=1507790237_51b8942c86707cf5e0a121fb628967df 10.1016/j.jfda.2016.09.007
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Easmin, Sabina Sarker, Md. Zaidul Islam Ghafoor, Kashif Ferdosh, Sahena Md. Jaffri, Juliana Ali, Md. Eaqub Mirhosseini, Hamed Al-Juhaimi, Fahad Y. Perumal, Vikneswari Khatib, Alfi Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting
description	Phaleria macrocarpa, known as “Mahkota Dewa”, is a widely used medicinal plant in Malaysia. This study focused on the characterization of α-glucosidase inhibitory activity of P. macrocarpa extracts using Fourier transform infrared spectroscopy (FTIR)-based metabolomics. P. macrocarpa and its extracts contain thousands of compounds having synergistic effect. Generally, their variability exists, and there are many active components in meager amounts. Thus, the conventional measurement methods of a single component for the quality control are time consuming, laborious, expensive, and unreliable. It is of great interest to develop a rapid prediction method for herbal quality control to investigate the α-glucosidase inhibitory activity of P. macrocarpa by multicomponent analyses. In this study, a rapid and simple analytical method was developed using FTIR spectroscopy-based fingerprinting. A total of 36 extracts of different ethanol concentrations were prepared and tested on inhibitory potential and fingerprinted using FTIR spectroscopy, coupled with chemometrics of orthogonal partial least square (OPLS) at the 4000–400 cm−1 frequency region and resolution of 4 cm−1. The OPLS model generated the highest regression coefficient with R2Y = 0.98 and Q2Y = 0.70, lowest root mean square error estimation = 17.17, and root mean square error of cross validation = 57.29. A five-component (1+4+0) predictive model was build up to correlate FTIR spectra with activity, and the responsible functional groups, such as –CH, –NH, –COOH, and –OH, were identified for the bioactivity. A successful multivariate model was constructed using FTIR-attenuated total reflection as a simple and rapid technique to predict the inhibitory activity.
format	Article
author	Easmin, Sabina Sarker, Md. Zaidul Islam Ghafoor, Kashif Ferdosh, Sahena Md. Jaffri, Juliana Ali, Md. Eaqub Mirhosseini, Hamed Al-Juhaimi, Fahad Y. Perumal, Vikneswari Khatib, Alfi
author_facet	Easmin, Sabina Sarker, Md. Zaidul Islam Ghafoor, Kashif Ferdosh, Sahena Md. Jaffri, Juliana Ali, Md. Eaqub Mirhosseini, Hamed Al-Juhaimi, Fahad Y. Perumal, Vikneswari Khatib, Alfi
author_sort	Easmin, Sabina
title	Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting
title_short	Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting
title_full	Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting
title_fullStr	Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting
title_full_unstemmed	Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting
title_sort	rapid investigation of a-glucosidase inhibitory activity of phaleria macrocarpa extracts using ftir-atr based fingerprinting
publisher	Elsevier
publishDate	2017
url	http://irep.iium.edu.my/53769/ http://irep.iium.edu.my/53769/ http://irep.iium.edu.my/53769/ http://irep.iium.edu.my/53769/12/53769-Rapid%20investigation.pdf http://irep.iium.edu.my/53769/13/53769-Rapid%20investigation_SCOPUS.pdf http://irep.iium.edu.my/53769/24/53769_Rapid%20investigation%20of%20%CE%B1-glucosidase_WOS.pdf
first_indexed	2023-09-18T21:16:03Z
last_indexed	2023-09-18T21:16:03Z
_version_	1777411569397792768

Rapid investigation of a-glucosidase inhibitory activity of Phaleria macrocarpa extracts using FTIR-ATR based fingerprinting

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