Molecular cloning and optimization for high level expression of cold-adapted serine protease from antarctic yeast glaciozyma antarctica PI12
Psychrophilic basidiomycete yeast, Glaciozyma antarctica strain PI12, was shown to be a protease-producer. Isolation of the PI12 protease gene from genomic and mRNA sequences allowed determination of 19 exons and 18 introns. Full-length cDNA of PI12 protease gene was amplified by rapid amplification...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Hindawi Publishing Corporation
2014
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/53050/ http://irep.iium.edu.my/53050/ http://irep.iium.edu.my/53050/ http://irep.iium.edu.my/53050/1/197938.pdf |