Pneumococcal hyaluronidase: a product of enzyme in normal human flora

Hyaluronidase degrades hyaluronan or hyaluronic acid (HA), a major component of extracellular matrix in human connective tissues. Among three types of hyaluronidase family members, hyaluroglucosaminidase which is extracted from vertebrate testes has been widely used in various medical or cosmeticall...

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Main Authors:	Ahmad Yusof @ Hanafi, Hanani, Mohd Desa, Mohd Nasir, Masri, Siti Norbaya, Osman, Malina, Jamal, Farida
Format:	Conference or Workshop Item
Language:	English
Published:	2016
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/52880/ http://irep.iium.edu.my/52880/23/52880.pdf

id	iium-52880
recordtype	eprints
spelling	iium-528802017-02-21T07:27:02Z http://irep.iium.edu.my/52880/ Pneumococcal hyaluronidase: a product of enzyme in normal human flora Ahmad Yusof @ Hanafi, Hanani Mohd Desa, Mohd Nasir Masri, Siti Norbaya Osman, Malina Jamal, Farida QR Microbiology Hyaluronidase degrades hyaluronan or hyaluronic acid (HA), a major component of extracellular matrix in human connective tissues. Among three types of hyaluronidase family members, hyaluroglucosaminidase which is extracted from vertebrate testes has been widely used in various medical or cosmetically-related procedures such as ophthalmic surgery and dermatosurgery. Animal-based proteins are often associated with the issue of halal status due either to their source or the production process. Bacteria can be an alternative source of enzyme and protein as no halal issues may be involved as in the case of animal products. Bacterial hyaluronidase has been reported to be produced by various species of microorganisms, including Streptococcus pneumoniae. Most previous studies reported pathogenic pneumococci as highly active producers of hyaluronidase, while its production by normal flora pneumococci has yet to be intensively explored. The present study attempted to compare hyaluronidase activity between normal flora and pathogenic pneumococci. A total of fifteen (n=15) pathogenic pneumococci isolated from blood of invasive pneumococcal disease patient and eleven (n=11) normal flora pneumococci isolated from anterior nares of healthy individuals were screened for hyaluronidase activity using HA agar plate and HA turbidity reduction assay. All pneumococci strains produced hyaluronidase, with higher enzymatic activity in normal flora pneumococci (p = 0.024). This finding suggests that normal flora could be a selected and studied for large scale production of hyaluronidase which more likely to be a syariah compliant and halal source of the enzyme. 2016 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/52880/23/52880.pdf Ahmad Yusof @ Hanafi, Hanani and Mohd Desa, Mohd Nasir and Masri, Siti Norbaya and Osman, Malina and Jamal, Farida (2016) Pneumococcal hyaluronidase: a product of enzyme in normal human flora. In: 2nd World Congress on Integration and Islamicisation: Focus on Medical and Healthcare Science (2WCII 2016), 21st-23rd October 2016, Kuantan, Pahang. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Ahmad Yusof @ Hanafi, Hanani Mohd Desa, Mohd Nasir Masri, Siti Norbaya Osman, Malina Jamal, Farida Pneumococcal hyaluronidase: a product of enzyme in normal human flora
description	Hyaluronidase degrades hyaluronan or hyaluronic acid (HA), a major component of extracellular matrix in human connective tissues. Among three types of hyaluronidase family members, hyaluroglucosaminidase which is extracted from vertebrate testes has been widely used in various medical or cosmetically-related procedures such as ophthalmic surgery and dermatosurgery. Animal-based proteins are often associated with the issue of halal status due either to their source or the production process. Bacteria can be an alternative source of enzyme and protein as no halal issues may be involved as in the case of animal products. Bacterial hyaluronidase has been reported to be produced by various species of microorganisms, including Streptococcus pneumoniae. Most previous studies reported pathogenic pneumococci as highly active producers of hyaluronidase, while its production by normal flora pneumococci has yet to be intensively explored. The present study attempted to compare hyaluronidase activity between normal flora and pathogenic pneumococci. A total of fifteen (n=15) pathogenic pneumococci isolated from blood of invasive pneumococcal disease patient and eleven (n=11) normal flora pneumococci isolated from anterior nares of healthy individuals were screened for hyaluronidase activity using HA agar plate and HA turbidity reduction assay. All pneumococci strains produced hyaluronidase, with higher enzymatic activity in normal flora pneumococci (p = 0.024). This finding suggests that normal flora could be a selected and studied for large scale production of hyaluronidase which more likely to be a syariah compliant and halal source of the enzyme.
format	Conference or Workshop Item
author	Ahmad Yusof @ Hanafi, Hanani Mohd Desa, Mohd Nasir Masri, Siti Norbaya Osman, Malina Jamal, Farida
author_facet	Ahmad Yusof @ Hanafi, Hanani Mohd Desa, Mohd Nasir Masri, Siti Norbaya Osman, Malina Jamal, Farida
author_sort	Ahmad Yusof @ Hanafi, Hanani
title	Pneumococcal hyaluronidase: a product of enzyme in normal human flora
title_short	Pneumococcal hyaluronidase: a product of enzyme in normal human flora
title_full	Pneumococcal hyaluronidase: a product of enzyme in normal human flora
title_fullStr	Pneumococcal hyaluronidase: a product of enzyme in normal human flora
title_full_unstemmed	Pneumococcal hyaluronidase: a product of enzyme in normal human flora
title_sort	pneumococcal hyaluronidase: a product of enzyme in normal human flora
publishDate	2016
url	http://irep.iium.edu.my/52880/ http://irep.iium.edu.my/52880/23/52880.pdf
first_indexed	2023-09-18T21:14:54Z
last_indexed	2023-09-18T21:14:54Z
_version_	1777411496933851136

Pneumococcal hyaluronidase: a product of enzyme in normal human flora

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