Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques

The distinctive fragrance and pleasing odor of the agarwood came from the resinous materials impregnated in the phloem of the tree as a response to infection. Artificial inoculants have been developed by ?trial and error? methods to boost the resin formation. These artificial inoculants have been us...

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Main Authors:	Nuge, Tamrin, Hashim, Yumi Zuhanis Has-Yun, Amid, Azura, Jaswir, Irwandi, Jamal, Parveen, Mohd Salleh, Hamzah
Format:	Article
Language:	English English
Published:	Journal of Pure and Applied Microbiology 2014
Subjects:	Q Science (General)
Online Access:	http://irep.iium.edu.my/51372/ http://irep.iium.edu.my/51372/3/51372.pdf http://irep.iium.edu.my/51372/6/51372_Identification%20of%20microbes%20in%20inoculant.SCOPUSpdf.pdf

id	iium-51372
recordtype	eprints
spelling	iium-513722017-12-21T07:56:18Z http://irep.iium.edu.my/51372/ Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques Nuge, Tamrin Hashim, Yumi Zuhanis Has-Yun Amid, Azura Jaswir, Irwandi Jamal, Parveen Mohd Salleh, Hamzah Q Science (General) The distinctive fragrance and pleasing odor of the agarwood came from the resinous materials impregnated in the phloem of the tree as a response to infection. Artificial inoculants have been developed by ?trial and error? methods to boost the resin formation. These artificial inoculants have been used widely in this region despite no strong scientific data and that they contribute to most of the cost for agarwood resin production. In collaboration with an agarwood industry player, a research work was initiated to isolate and identify the microorganisms present in an inoculant that has been proven effective in boosting the agarwood resin formation. Fifteen clones were constructed by extracting and amplifying the 16S rDNA and ITS sequences from the pure cultures isolated from the inoculant. Three genera of microorganisms (Bacillus, Paenabacillus, Monascus) have been successfully identified. Two bacteria clones were phylogenetically affiliated with Paenibacillus sp. and four clones were associated with Bacillus sp. as well as three fungi clones were associated with Monascus sp. All the identified species are spore-forming microorganisms. The genera of Bacillus and Paenabacillus were able to utilize carbohydrate and protein from the tree as their energy sources. These two genera may be crucial to boost resin formation in agarwood. Journal of Pure and Applied Microbiology 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51372/3/51372.pdf application/pdf en http://irep.iium.edu.my/51372/6/51372_Identification%20of%20microbes%20in%20inoculant.SCOPUSpdf.pdf Nuge, Tamrin and Hashim, Yumi Zuhanis Has-Yun and Amid, Azura and Jaswir, Irwandi and Jamal, Parveen and Mohd Salleh, Hamzah (2014) Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques. Journal of Pure and Applied Microbiology, 8 (May (Special edition)). pp. 769-776. ISSN 0973-7510
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Nuge, Tamrin Hashim, Yumi Zuhanis Has-Yun Amid, Azura Jaswir, Irwandi Jamal, Parveen Mohd Salleh, Hamzah Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques
description	The distinctive fragrance and pleasing odor of the agarwood came from the resinous materials impregnated in the phloem of the tree as a response to infection. Artificial inoculants have been developed by ?trial and error? methods to boost the resin formation. These artificial inoculants have been used widely in this region despite no strong scientific data and that they contribute to most of the cost for agarwood resin production. In collaboration with an agarwood industry player, a research work was initiated to isolate and identify the microorganisms present in an inoculant that has been proven effective in boosting the agarwood resin formation. Fifteen clones were constructed by extracting and amplifying the 16S rDNA and ITS sequences from the pure cultures isolated from the inoculant. Three genera of microorganisms (Bacillus, Paenabacillus, Monascus) have been successfully identified. Two bacteria clones were phylogenetically affiliated with Paenibacillus sp. and four clones were associated with Bacillus sp. as well as three fungi clones were associated with Monascus sp. All the identified species are spore-forming microorganisms. The genera of Bacillus and Paenabacillus were able to utilize carbohydrate and protein from the tree as their energy sources. These two genera may be crucial to boost resin formation in agarwood.
format	Article
author	Nuge, Tamrin Hashim, Yumi Zuhanis Has-Yun Amid, Azura Jaswir, Irwandi Jamal, Parveen Mohd Salleh, Hamzah
author_facet	Nuge, Tamrin Hashim, Yumi Zuhanis Has-Yun Amid, Azura Jaswir, Irwandi Jamal, Parveen Mohd Salleh, Hamzah
author_sort	Nuge, Tamrin
title	Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques
title_short	Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques
title_full	Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques
title_fullStr	Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques
title_full_unstemmed	Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques
title_sort	identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques
publisher	Journal of Pure and Applied Microbiology
publishDate	2014
url	http://irep.iium.edu.my/51372/ http://irep.iium.edu.my/51372/3/51372.pdf http://irep.iium.edu.my/51372/6/51372_Identification%20of%20microbes%20in%20inoculant.SCOPUSpdf.pdf
first_indexed	2023-09-18T21:12:43Z
last_indexed	2023-09-18T21:12:43Z
_version_	1777411359919570944

Identification of microbes in inoculant for agarwood (gaharu) formation using molecular biological techniques

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