Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity

Lycopene, one of the most widely used carotenoid is an efficient antioxidant and singlet oxygen quencher. Increasing demand of lycopene in the nutraceutical and drug industry has directed the researchers to produce lycopene with cost effective methods in large scale to meet the growing demand. The...

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Main Authors:	Jamal, Parveen, Akbar, Iqrah, Hashim, Yumi Zuhanis Has-Yun, Jaswir, Irwandi
Format:	Article
Language:	English
Published:	OMICS International 2016
Subjects:	T Technology (General) TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/51248/ http://irep.iium.edu.my/51248/ http://irep.iium.edu.my/51248/ http://irep.iium.edu.my/51248/1/Parveen_Iqra_paper.pdf

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recordtype	eprints
spelling	iium-512482016-07-26T08:44:45Z http://irep.iium.edu.my/51248/ Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity Jamal, Parveen Akbar, Iqrah Hashim, Yumi Zuhanis Has-Yun Jaswir, Irwandi T Technology (General) TP248.13 Biotechnology Lycopene, one of the most widely used carotenoid is an efficient antioxidant and singlet oxygen quencher. Increasing demand of lycopene in the nutraceutical and drug industry has directed the researchers to produce lycopene with cost effective methods in large scale to meet the growing demand. Thermal processing liberates this carotenoid from complexes with proteins and thus increases its bioaccessibilty. The comparison of lycopene content was explored amongst four fruit peels; guava, papaya, watermelon and red dragon fruit to choose for the best source. Lycopene content was measured using both UV–vis spectrophotometer and identified using high performance liquid chromatography (HPLC) Papaya, a tropical fruit showed tremendous potential as an alternative source and was selected to conduct further investigation. Response surface methodology (RSM) using faced centered composite design (FCCCD) was applied to study the interaction between the most contributing factors i.e., temperature, time and solid-solvent ratio with maximum lycopene yield of 103.1 mg/kg, the DPPH and FRAP equals to 81.85% and 836.46 μM Fe (II)/L respectively and a higher TPC of 1735.1 mg/L GAE at temperature 120°C, time of 5 hours in a solid-solvent ratio of 1:40 g/ ml. While lycopene yield of 74.538 mg/ kg exhibits DPPH scavenging activity of 91.14%; FRAP value of 954 μM Fe(II)/L and TPC content equals 1409.42 mg/L GAE at a temperature of 120°C for 4 hours extraction time with a solid-solvent ratio of 1:30 g/ml. The lycopene oleoresin was saponified using a mixture of propylene glycol and aqueous alkali to obtain lycopene crystals. The substantially pure lycopene crystals so obtained are fit for human consumption and were identified with High Performance Liquid Chromatography revealing that the major constituents of the lycopene oleoresin after saponification were lycopene and β-carotene which constitute 69.879% and 30.121% of the total oleoresin respectively. OMICS International 2016-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/51248/1/Parveen_Iqra_paper.pdf Jamal, Parveen and Akbar, Iqrah and Hashim, Yumi Zuhanis Has-Yun and Jaswir, Irwandi (2016) Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity. Journal of Food Processing & Technology, 7 (4). pp. 1-7. ISSN 2157-7110 http://www.omicsonline.org/open-access/process-development-for-maximum-lycopene-production-from-selected-fruit-waste-and-its-antioxidant-and-antiradical-activity-2157-7110-1000576.pdf 10.4172/2157-7110.1000576
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	T Technology (General) TP248.13 Biotechnology
spellingShingle	T Technology (General) TP248.13 Biotechnology Jamal, Parveen Akbar, Iqrah Hashim, Yumi Zuhanis Has-Yun Jaswir, Irwandi Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity
description	Lycopene, one of the most widely used carotenoid is an efficient antioxidant and singlet oxygen quencher. Increasing demand of lycopene in the nutraceutical and drug industry has directed the researchers to produce lycopene with cost effective methods in large scale to meet the growing demand. Thermal processing liberates this carotenoid from complexes with proteins and thus increases its bioaccessibilty. The comparison of lycopene content was explored amongst four fruit peels; guava, papaya, watermelon and red dragon fruit to choose for the best source. Lycopene content was measured using both UV–vis spectrophotometer and identified using high performance liquid chromatography (HPLC) Papaya, a tropical fruit showed tremendous potential as an alternative source and was selected to conduct further investigation. Response surface methodology (RSM) using faced centered composite design (FCCCD) was applied to study the interaction between the most contributing factors i.e., temperature, time and solid-solvent ratio with maximum lycopene yield of 103.1 mg/kg, the DPPH and FRAP equals to 81.85% and 836.46 μM Fe (II)/L respectively and a higher TPC of 1735.1 mg/L GAE at temperature 120°C, time of 5 hours in a solid-solvent ratio of 1:40 g/ ml. While lycopene yield of 74.538 mg/ kg exhibits DPPH scavenging activity of 91.14%; FRAP value of 954 μM Fe(II)/L and TPC content equals 1409.42 mg/L GAE at a temperature of 120°C for 4 hours extraction time with a solid-solvent ratio of 1:30 g/ml. The lycopene oleoresin was saponified using a mixture of propylene glycol and aqueous alkali to obtain lycopene crystals. The substantially pure lycopene crystals so obtained are fit for human consumption and were identified with High Performance Liquid Chromatography revealing that the major constituents of the lycopene oleoresin after saponification were lycopene and β-carotene which constitute 69.879% and 30.121% of the total oleoresin respectively.
format	Article
author	Jamal, Parveen Akbar, Iqrah Hashim, Yumi Zuhanis Has-Yun Jaswir, Irwandi
author_facet	Jamal, Parveen Akbar, Iqrah Hashim, Yumi Zuhanis Has-Yun Jaswir, Irwandi
author_sort	Jamal, Parveen
title	Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity
title_short	Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity
title_full	Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity
title_fullStr	Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity
title_full_unstemmed	Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity
title_sort	process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity
publisher	OMICS International
publishDate	2016
url	http://irep.iium.edu.my/51248/ http://irep.iium.edu.my/51248/ http://irep.iium.edu.my/51248/ http://irep.iium.edu.my/51248/1/Parveen_Iqra_paper.pdf
first_indexed	2023-09-18T21:12:32Z
last_indexed	2023-09-18T21:12:32Z
_version_	1777411348535181312

Process development for maximum lycopene production from selected fruit waste and its antioxidant and antiradical activity

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