Bioethanol production from sago starch
Two stage processes prior to ethanol production has been proposed which is hydrolysis and fermentation. Commercial enzymes were used in this two steps hydrolysis; α-amylase (liquefaction step) and glucoamylase (saccharification step). Optimization was carried out in both stages; hydrolysis and ferme...
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iium-50442012-02-27T09:01:50Z http://irep.iium.edu.my/5044/ Bioethanol production from sago starch Mel, Maizirwan Muhammad Nadzri, Husna Kamarudin, Mohd Hider Abd Karim, Mohd Ismail TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol Two stage processes prior to ethanol production has been proposed which is hydrolysis and fermentation. Commercial enzymes were used in this two steps hydrolysis; α-amylase (liquefaction step) and glucoamylase (saccharification step). Optimization was carried out in both stages; hydrolysis and fermentation. Three parameters are involved in optimization of % dextrose equivalent (DE); sago starch concentration (20% (w/v), 30% (w/v), 40% (w/v)), glucoamylase enzyme (52 U/g, 78 U/g, 104 U/g) and time during saccharification (1, 2, 3 hours). Three parameters are involved in optimization of ethanol; agitation (100 rpm, 150 rpm, 200 rpm), inoculums (1% (v/v), 3% (v/v), 5% (v/v)) from constant initial stock of 2.5x106 cells/mL and pH (4, 5, 6). Both optimization studies were carried out using Box-Behnken design. The experiment showed that the optimum parameters for hydrolysis study was identified to be glucoamylase (75.87 U/g), substrate concentration (28.49% (w/v)), and time (2 hours) which produced 62.15 g/L glucose as the fermentation substrate. For ethanol fermentation study, it was identified that the optimum parameters that produced 29.25 g/L were 167 rpm agitation, 3.43% (v/v) inoculums and pH 5. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/5044/1/Ethanol_from_Sago-Paper_for_ICT-NRE_Jakarta_2010_%28edited_20_Oct_2010%29.pdf Mel, Maizirwan and Muhammad Nadzri, Husna and Kamarudin, Mohd Hider and Abd Karim, Mohd Ismail (2010) Bioethanol production from sago starch. In: International Conference on Technology for New and Renewable Energy (ICT-NRE), 1 - 3 December, 2010, Jakarta Indonesia. |
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International Islamic University Malaysia |
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TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol |
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TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol Mel, Maizirwan Muhammad Nadzri, Husna Kamarudin, Mohd Hider Abd Karim, Mohd Ismail Bioethanol production from sago starch |
description |
Two stage processes prior to ethanol production has been proposed which is hydrolysis and fermentation. Commercial enzymes were used in this two steps hydrolysis; α-amylase (liquefaction step) and glucoamylase (saccharification step). Optimization was carried out in both stages; hydrolysis and fermentation. Three parameters are involved in optimization of % dextrose equivalent (DE); sago starch concentration (20% (w/v), 30% (w/v), 40% (w/v)), glucoamylase enzyme (52 U/g, 78 U/g, 104 U/g) and time during saccharification (1, 2, 3 hours). Three parameters are involved in optimization of ethanol; agitation (100 rpm, 150 rpm, 200 rpm), inoculums (1% (v/v), 3% (v/v), 5% (v/v)) from constant initial stock of 2.5x106 cells/mL and pH (4, 5, 6). Both optimization studies were carried out using Box-Behnken design. The experiment showed that the optimum parameters for hydrolysis study was identified to be glucoamylase (75.87 U/g), substrate concentration (28.49% (w/v)), and time (2 hours) which produced 62.15 g/L glucose as the fermentation substrate. For ethanol fermentation study, it was identified that the optimum parameters that produced 29.25 g/L were 167 rpm agitation, 3.43% (v/v) inoculums and pH 5. |
format |
Conference or Workshop Item |
author |
Mel, Maizirwan Muhammad Nadzri, Husna Kamarudin, Mohd Hider Abd Karim, Mohd Ismail |
author_facet |
Mel, Maizirwan Muhammad Nadzri, Husna Kamarudin, Mohd Hider Abd Karim, Mohd Ismail |
author_sort |
Mel, Maizirwan |
title |
Bioethanol production from sago starch |
title_short |
Bioethanol production from sago starch |
title_full |
Bioethanol production from sago starch |
title_fullStr |
Bioethanol production from sago starch |
title_full_unstemmed |
Bioethanol production from sago starch |
title_sort |
bioethanol production from sago starch |
publishDate |
2010 |
url |
http://irep.iium.edu.my/5044/ http://irep.iium.edu.my/5044/1/Ethanol_from_Sago-Paper_for_ICT-NRE_Jakarta_2010_%28edited_20_Oct_2010%29.pdf |
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2023-09-18T20:13:31Z |
last_indexed |
2023-09-18T20:13:31Z |
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1777407635727843328 |