Bioethanol production from sago starch

Two stage processes prior to ethanol production has been proposed which is hydrolysis and fermentation. Commercial enzymes were used in this two steps hydrolysis; α-amylase (liquefaction step) and glucoamylase (saccharification step). Optimization was carried out in both stages; hydrolysis and ferme...

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Main Authors: Mel, Maizirwan, Muhammad Nadzri, Husna, Kamarudin, Mohd Hider, Abd Karim, Mohd Ismail
Format: Conference or Workshop Item
Language:English
Published: 2010
Subjects:
Online Access:http://irep.iium.edu.my/5044/
http://irep.iium.edu.my/5044/1/Ethanol_from_Sago-Paper_for_ICT-NRE_Jakarta_2010_%28edited_20_Oct_2010%29.pdf
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recordtype eprints
spelling iium-50442012-02-27T09:01:50Z http://irep.iium.edu.my/5044/ Bioethanol production from sago starch Mel, Maizirwan Muhammad Nadzri, Husna Kamarudin, Mohd Hider Abd Karim, Mohd Ismail TP155 Chemical engineering TP248.13 Biotechnology TP315 Fuel TP500 Fermentation industries. Beverages. Alcohol Two stage processes prior to ethanol production has been proposed which is hydrolysis and fermentation. Commercial enzymes were used in this two steps hydrolysis; α-amylase (liquefaction step) and glucoamylase (saccharification step). Optimization was carried out in both stages; hydrolysis and fermentation. Three parameters are involved in optimization of % dextrose equivalent (DE); sago starch concentration (20% (w/v), 30% (w/v), 40% (w/v)), glucoamylase enzyme (52 U/g, 78 U/g, 104 U/g) and time during saccharification (1, 2, 3 hours). Three parameters are involved in optimization of ethanol; agitation (100 rpm, 150 rpm, 200 rpm), inoculums (1% (v/v), 3% (v/v), 5% (v/v)) from constant initial stock of 2.5x106 cells/mL and pH (4, 5, 6). Both optimization studies were carried out using Box-Behnken design. The experiment showed that the optimum parameters for hydrolysis study was identified to be glucoamylase (75.87 U/g), substrate concentration (28.49% (w/v)), and time (2 hours) which produced 62.15 g/L glucose as the fermentation substrate. For ethanol fermentation study, it was identified that the optimum parameters that produced 29.25 g/L were 167 rpm agitation, 3.43% (v/v) inoculums and pH 5. 2010 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/5044/1/Ethanol_from_Sago-Paper_for_ICT-NRE_Jakarta_2010_%28edited_20_Oct_2010%29.pdf Mel, Maizirwan and Muhammad Nadzri, Husna and Kamarudin, Mohd Hider and Abd Karim, Mohd Ismail (2010) Bioethanol production from sago starch. In: International Conference on Technology for New and Renewable Energy (ICT-NRE), 1 - 3 December, 2010, Jakarta Indonesia.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic TP155 Chemical engineering
TP248.13 Biotechnology
TP315 Fuel
TP500 Fermentation industries. Beverages. Alcohol
spellingShingle TP155 Chemical engineering
TP248.13 Biotechnology
TP315 Fuel
TP500 Fermentation industries. Beverages. Alcohol
Mel, Maizirwan
Muhammad Nadzri, Husna
Kamarudin, Mohd Hider
Abd Karim, Mohd Ismail
Bioethanol production from sago starch
description Two stage processes prior to ethanol production has been proposed which is hydrolysis and fermentation. Commercial enzymes were used in this two steps hydrolysis; α-amylase (liquefaction step) and glucoamylase (saccharification step). Optimization was carried out in both stages; hydrolysis and fermentation. Three parameters are involved in optimization of % dextrose equivalent (DE); sago starch concentration (20% (w/v), 30% (w/v), 40% (w/v)), glucoamylase enzyme (52 U/g, 78 U/g, 104 U/g) and time during saccharification (1, 2, 3 hours). Three parameters are involved in optimization of ethanol; agitation (100 rpm, 150 rpm, 200 rpm), inoculums (1% (v/v), 3% (v/v), 5% (v/v)) from constant initial stock of 2.5x106 cells/mL and pH (4, 5, 6). Both optimization studies were carried out using Box-Behnken design. The experiment showed that the optimum parameters for hydrolysis study was identified to be glucoamylase (75.87 U/g), substrate concentration (28.49% (w/v)), and time (2 hours) which produced 62.15 g/L glucose as the fermentation substrate. For ethanol fermentation study, it was identified that the optimum parameters that produced 29.25 g/L were 167 rpm agitation, 3.43% (v/v) inoculums and pH 5.
format Conference or Workshop Item
author Mel, Maizirwan
Muhammad Nadzri, Husna
Kamarudin, Mohd Hider
Abd Karim, Mohd Ismail
author_facet Mel, Maizirwan
Muhammad Nadzri, Husna
Kamarudin, Mohd Hider
Abd Karim, Mohd Ismail
author_sort Mel, Maizirwan
title Bioethanol production from sago starch
title_short Bioethanol production from sago starch
title_full Bioethanol production from sago starch
title_fullStr Bioethanol production from sago starch
title_full_unstemmed Bioethanol production from sago starch
title_sort bioethanol production from sago starch
publishDate 2010
url http://irep.iium.edu.my/5044/
http://irep.iium.edu.my/5044/1/Ethanol_from_Sago-Paper_for_ICT-NRE_Jakarta_2010_%28edited_20_Oct_2010%29.pdf
first_indexed 2023-09-18T20:13:31Z
last_indexed 2023-09-18T20:13:31Z
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