Screening of various parts of phaleria macrocarpa plant for α-glucosidase inhibitory activity
Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α -glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa . Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room tempera...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English English English |
| Published: |
Wiley-Blackwell
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/46843/ http://irep.iium.edu.my/46843/ http://irep.iium.edu.my/46843/ http://irep.iium.edu.my/46843/1/46843_Screening%20of%20various%20parts%20of%20phaleria%20macrocarpa.pdf http://irep.iium.edu.my/46843/2/46843_Screening%20of%20various%20parts%20of%20phaleria%20macrocarpa_WOS.pdf http://irep.iium.edu.my/46843/3/46843_Screening%20of%20various%20parts%20of%20phaleria%20macrocarpa_SCOPUS.pdf |
| Summary: | Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the
α -glucosidase inhibitory activity of different
parts (fruit flesh, leaves and stem) of
P. macrocarpa . Methanol (polar) and
n-hexane (nonpolar) extracts, obtained by room temperature solvent extraction, were evaluated for in vitro
α-glucosidase activity inhibition. The compounds were
identified by using gas chromatography-mass spectrometry (GC-MS) according to their similarity index of >70%, which might be responsible for α-glucosidase
inhibitory activity. The methanol extract of the fruit flesh had the highest yield (25.6±0.5%), whereas the n-hexane extract of the stem is more effective against
α-glucosidase activity (IC500.8±0.1μg/mL). The fruit flesh (IC501.3±0.2μg/mL)and leaves (IC501.6±0.6μg/mL) had also well effectively. The identified metabo-lites are predominantly phenolics, carbohydrates, triterpenes and organic acids,such as D-fructose, squalene,α
-linolenic acid and α-D-glucopyranoside. In-depth
chemical profiling using GC-MS was performed for the first time for this plant to assess the likely compounds present in the extract that could be associated with
anti-hyperglycemic activity. Of the three parts tested, every part indicates the potential α-glucosidase inhibitory activity and hexane extract of stem showed more inhibitory activity among all extracts. Thus,P. macrocarpa can attenuate hyperglycemia by potently inhibiting carbohydrate hydrolyzing enzymes, making it a viable plant as a source of natural compounds for the management of type 2
diabetes mellitus. |
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