Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids.
A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15- diepoxyeicosanoate) were isolated from the matrix by transe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English English |
| Published: |
Penerbit UKM
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/43217/ http://irep.iium.edu.my/43217/ http://irep.iium.edu.my/43217/1/sains_malaysiana.pdf http://irep.iium.edu.my/43217/4/43217_Development%20and%20validation%20of%20gas_SCOPUS.pdf |
| Summary: | A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with
metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15-
diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good
linear dynamic range between 0.7-20 µg mL-1 with coefficients of determination (R2
) > 0.9968 and acceptable limit of
detection and limit of quantitation of 7 and 23 mg kg-1, respectively, based on linearity calculations. Analyte recoveries
were 90.84±27.24% for low concentration, 78.05±11.59% for medium concentration and 99.23±10.20% for high
concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods
stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific
migration limit of 60 mg kg-1 (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for
routine analysis for the determination of ESBO |
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