Methylation specific-high resolution melting analysis of DISC1

Introduction: The evidence of Disrupted in Schizophrenia 1 (DISC1) gene’s role in schizophrenia came through functional and animal studies. However the exact genetic basis that contribute to the role is still not established. As meta-analyses findings have denied DISC1 variants as the genetic basis...

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Main Authors: Abd. Rahim, Nour El Huda, Ku Zaifah, Norsidah, A.Talib, Norlelawati
Format: Conference or Workshop Item
Language:English
Published: 2014
Subjects:
Online Access:http://irep.iium.edu.my/40632/
http://irep.iium.edu.my/40632/
http://irep.iium.edu.my/40632/1/Binder1.pdf
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recordtype eprints
spelling iium-406322018-05-23T06:17:40Z http://irep.iium.edu.my/40632/ Methylation specific-high resolution melting analysis of DISC1 Abd. Rahim, Nour El Huda Ku Zaifah, Norsidah A.Talib, Norlelawati QH426 Genetics R Medicine (General) Introduction: The evidence of Disrupted in Schizophrenia 1 (DISC1) gene’s role in schizophrenia came through functional and animal studies. However the exact genetic basis that contribute to the role is still not established. As meta-analyses findings have denied DISC1 variants as the genetic basis, emphasis is now shifted to genetic defect such as copy number and epigenetic which have not been well explored. Objectives: We aim to explore the epigenetic dysregulation as the basis of DISC1 gene contribution to schizophrenia. To achieve this aim, method for quantitation of DISC1’s DNA methylation has to be established. Methods: The high resolution melting (HRM) analysis method was adopted for DNA methylation assay. Primers were designed based on Wojdacz’s protocol with some modifications. The human methylated and unmethylated control DNA samples were titrated into serial methylation percentage of 0%, 25%, 50%, 75% and 100% and were bisulfite treated. The methylation specific-high resolution melting (MS-HRM) assay for DISC1 was optimized based on its response to various percentage of DNA methylation. Results: The optimized protocol clearly demonstrated the serial methylated percentage differentiation of 0%, 25%, 50%, 75% and 100% accordingly. Conclusion: This optimized HRM protocol is to be used for the actual MS-HRM analysis of the DISC1 in schizophrenia. 2014-12-03 Conference or Workshop Item NonPeerReviewed application/pdf en http://irep.iium.edu.my/40632/1/Binder1.pdf Abd. Rahim, Nour El Huda and Ku Zaifah, Norsidah and A.Talib, Norlelawati (2014) Methylation specific-high resolution melting analysis of DISC1. In: International Medical Genetics Conference (IMGC 2014), 3-5 December 2014, Putra World Trade Centre. (Unpublished) http://imgc2014.com/
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QH426 Genetics
R Medicine (General)
spellingShingle QH426 Genetics
R Medicine (General)
Abd. Rahim, Nour El Huda
Ku Zaifah, Norsidah
A.Talib, Norlelawati
Methylation specific-high resolution melting analysis of DISC1
description Introduction: The evidence of Disrupted in Schizophrenia 1 (DISC1) gene’s role in schizophrenia came through functional and animal studies. However the exact genetic basis that contribute to the role is still not established. As meta-analyses findings have denied DISC1 variants as the genetic basis, emphasis is now shifted to genetic defect such as copy number and epigenetic which have not been well explored. Objectives: We aim to explore the epigenetic dysregulation as the basis of DISC1 gene contribution to schizophrenia. To achieve this aim, method for quantitation of DISC1’s DNA methylation has to be established. Methods: The high resolution melting (HRM) analysis method was adopted for DNA methylation assay. Primers were designed based on Wojdacz’s protocol with some modifications. The human methylated and unmethylated control DNA samples were titrated into serial methylation percentage of 0%, 25%, 50%, 75% and 100% and were bisulfite treated. The methylation specific-high resolution melting (MS-HRM) assay for DISC1 was optimized based on its response to various percentage of DNA methylation. Results: The optimized protocol clearly demonstrated the serial methylated percentage differentiation of 0%, 25%, 50%, 75% and 100% accordingly. Conclusion: This optimized HRM protocol is to be used for the actual MS-HRM analysis of the DISC1 in schizophrenia.
format Conference or Workshop Item
author Abd. Rahim, Nour El Huda
Ku Zaifah, Norsidah
A.Talib, Norlelawati
author_facet Abd. Rahim, Nour El Huda
Ku Zaifah, Norsidah
A.Talib, Norlelawati
author_sort Abd. Rahim, Nour El Huda
title Methylation specific-high resolution melting analysis of DISC1
title_short Methylation specific-high resolution melting analysis of DISC1
title_full Methylation specific-high resolution melting analysis of DISC1
title_fullStr Methylation specific-high resolution melting analysis of DISC1
title_full_unstemmed Methylation specific-high resolution melting analysis of DISC1
title_sort methylation specific-high resolution melting analysis of disc1
publishDate 2014
url http://irep.iium.edu.my/40632/
http://irep.iium.edu.my/40632/
http://irep.iium.edu.my/40632/1/Binder1.pdf
first_indexed 2023-09-18T20:58:18Z
last_indexed 2023-09-18T20:58:18Z
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