Experimental design and statistical analysis of protein buffer to purify hydrolases from the skim latex of hevea brasiliensis
A lipid acyl hydrolase (LAH) enzyme has been purified from skim latex serum, a waste product of Hevea brasiliensis, using DEAE Sepharose™ Fast Flow ion exchange chromatography. The ~45kDa active LAH was expressed by spectrophotometric study and SDS-PAGE. One-Factor-at-A-Time (OFAT) method and Centra...
| Main Authors: | , |
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| Format: | Article |
| Language: | English English |
| Published: |
AENSI Publications
2014
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/ http://irep.iium.edu.my/39221/1/P_46.pdf http://irep.iium.edu.my/39221/4/39221_Experimental%20design%20and%20statistical_scopus.pdf |