Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed

Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed

A study on the anti-inflammatory role of ethanolic precipitates and supernatants of water soluble extracts of 5 brown seaweed species of Malaysian origin (Sargassum duplicatum, Sargassum binderi, Sargassum fulvellum, Padina australis and Turbinaria turbinata) on inhibition of Nitric oxide (NO) secr...

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Main Authors: Monsur, Hammed Ademola, Jaswir, Irwandi, Simsek, Senay, Amid, Azura, Alam, Md Zahangir, Tawakalit Tope, Asiyanbi-Hammed
Format: Article
Language:English
English
English
Published: Elsevier Inc. 2014
Subjects:
QK Botany
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/36623/
http://irep.iium.edu.my/36623/
http://irep.iium.edu.my/36623/
http://irep.iium.edu.my/36623/4/cytotoxicity.pdf
http://irep.iium.edu.my/36623/7/36623-Cytotoxicity%20and%20inhibition%20of%20nitric%20oxide%20syntheses%20in%20LPS%20induced%20macrophage_SCOPUS.pdf
http://irep.iium.edu.my/36623/8/36623-Cytotoxicity%20and%20inhibition%20of%20nitric%20oxide%20syntheses%20in%20LPS%20induced%20macrophage_WOS.pdf
id iium-36623
recordtype eprints
spelling iium-366232017-08-03T07:27:23Z http://irep.iium.edu.my/36623/ Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed Monsur, Hammed Ademola Jaswir, Irwandi Simsek, Senay Amid, Azura Alam, Md Zahangir Tawakalit Tope, Asiyanbi-Hammed QK Botany TP248.13 Biotechnology A study on the anti-inflammatory role of ethanolic precipitates and supernatants of water soluble extracts of 5 brown seaweed species of Malaysian origin (Sargassum duplicatum, Sargassum binderi, Sargassum fulvellum, Padina australis and Turbinaria turbinata) on inhibition of Nitric oxide (NO) secretion in lipopolysaccharide (LPS) induced RAW264.7 murine macrophage was conducted. Results showed that fractions obtained varied in yield and chemical compositions, such as uronic acid, sulfate ion, monosaccharide, polyphenol and total sugar. In terms of yield percentage, precipitate obtained from T. turbinata contained the highest, w25%. All precipitates contained reasonable amount of fucose, mannose, galactose, glucose and xylose, but trace amount of arabinose. The precipitates inhibited NO secretion in a dose dependent manner. Precipitate of P. australis and T. turbinata, dosed at 200 mg/ml, down-regulated (more than 80%) NO secretion. Among the supernates, that of T. turbinata, which contain highest fucose, gave highest inhibition of NO synthesis. Results also showed that all the extracts obtained gave more than 80% cell proliferation except the precipitate of S. duplicatum. Data from this study also indicated that water soluble extracts of seaweed can be explored for alternative anti-inflammatory drug. Elsevier Inc. 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36623/4/cytotoxicity.pdf application/pdf en http://irep.iium.edu.my/36623/7/36623-Cytotoxicity%20and%20inhibition%20of%20nitric%20oxide%20syntheses%20in%20LPS%20induced%20macrophage_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/36623/8/36623-Cytotoxicity%20and%20inhibition%20of%20nitric%20oxide%20syntheses%20in%20LPS%20induced%20macrophage_WOS.pdf Monsur, Hammed Ademola and Jaswir, Irwandi and Simsek, Senay and Amid, Azura and Alam, Md Zahangir and Tawakalit Tope, Asiyanbi-Hammed (2014) Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed. Food Hydrocolloids, 42. pp. 269-274. ISSN 0268-005X http://www.sciencedirect.com/science/article/pii/S0268005X1400071X 10.1016/j.foodhyd.2014.02.021
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
English
topic QK Botany
TP248.13 Biotechnology
spellingShingle QK Botany
TP248.13 Biotechnology
Monsur, Hammed Ademola
Jaswir, Irwandi
Simsek, Senay
Amid, Azura
Alam, Md Zahangir
Tawakalit Tope, Asiyanbi-Hammed
Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed
description A study on the anti-inflammatory role of ethanolic precipitates and supernatants of water soluble extracts of 5 brown seaweed species of Malaysian origin (Sargassum duplicatum, Sargassum binderi, Sargassum fulvellum, Padina australis and Turbinaria turbinata) on inhibition of Nitric oxide (NO) secretion in lipopolysaccharide (LPS) induced RAW264.7 murine macrophage was conducted. Results showed that fractions obtained varied in yield and chemical compositions, such as uronic acid, sulfate ion, monosaccharide, polyphenol and total sugar. In terms of yield percentage, precipitate obtained from T. turbinata contained the highest, w25%. All precipitates contained reasonable amount of fucose, mannose, galactose, glucose and xylose, but trace amount of arabinose. The precipitates inhibited NO secretion in a dose dependent manner. Precipitate of P. australis and T. turbinata, dosed at 200 mg/ml, down-regulated (more than 80%) NO secretion. Among the supernates, that of T. turbinata, which contain highest fucose, gave highest inhibition of NO synthesis. Results also showed that all the extracts obtained gave more than 80% cell proliferation except the precipitate of S. duplicatum. Data from this study also indicated that water soluble extracts of seaweed can be explored for alternative anti-inflammatory drug.
format Article
author Monsur, Hammed Ademola
Jaswir, Irwandi
Simsek, Senay
Amid, Azura
Alam, Md Zahangir
Tawakalit Tope, Asiyanbi-Hammed
author_facet Monsur, Hammed Ademola
Jaswir, Irwandi
Simsek, Senay
Amid, Azura
Alam, Md Zahangir
Tawakalit Tope, Asiyanbi-Hammed
author_sort Monsur, Hammed Ademola
title Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed
title_short Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed
title_full Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed
title_fullStr Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed
title_full_unstemmed Cytotoxicity and inhibition of nitric oxide syntheses in LPS induced macrophage by water soluble fractions of brown seaweed
title_sort cytotoxicity and inhibition of nitric oxide syntheses in lps induced macrophage by water soluble fractions of brown seaweed
publisher Elsevier Inc.
publishDate 2014
url http://irep.iium.edu.my/36623/
http://irep.iium.edu.my/36623/
http://irep.iium.edu.my/36623/
http://irep.iium.edu.my/36623/4/cytotoxicity.pdf
http://irep.iium.edu.my/36623/7/36623-Cytotoxicity%20and%20inhibition%20of%20nitric%20oxide%20syntheses%20in%20LPS%20induced%20macrophage_SCOPUS.pdf
http://irep.iium.edu.my/36623/8/36623-Cytotoxicity%20and%20inhibition%20of%20nitric%20oxide%20syntheses%20in%20LPS%20induced%20macrophage_WOS.pdf
first_indexed 2023-09-18T20:52:27Z
last_indexed 2023-09-18T20:52:27Z
_version_ 1777410084974886912

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