Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design

Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from...

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Main Authors:	Jamal, Parveen, Azmi, Saiful Mohammad Nizam, Amid, Azura, Mohd. Salleh, Hamzah, Hashim, Yumi Zuhanis Has-Yun
Format:	Article
Language:	English English
Published:	AENSI Publications 2014
Subjects:	T Technology (General) TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/36413/ http://irep.iium.edu.my/36413/ http://irep.iium.edu.my/36413/1/734-743_Parveen_.pdf http://irep.iium.edu.my/36413/4/36413_Development%20and%20improvement%20of%20anti-gout_SCOPUS.pdf

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spelling	iium-364132017-09-19T04:30:51Z http://irep.iium.edu.my/36413/ Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun T Technology (General) TP248.13 Biotechnology Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from the leaves of Morinda elliptica was performed by using 70% methanol. Optimization of process parameters such as extraction temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of sample to solvent (1g/ml) at five levels was carried out using central composite design (CCD) for the improvement of activity to treat gout. The analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity was improved at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized conditions were verified and the percentage of XO inhibitory activity obtained was 88.93%. The results are encouraging to formulate food, nutraceutical or pharmaceutical products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to irresponsive synthetic XOI. AENSI Publications 2014 Article PeerReviewed application/pdf en http://irep.iium.edu.my/36413/1/734-743_Parveen_.pdf application/pdf en http://irep.iium.edu.my/36413/4/36413_Development%20and%20improvement%20of%20anti-gout_SCOPUS.pdf Jamal, Parveen and Azmi, Saiful Mohammad Nizam and Amid, Azura and Mohd. Salleh, Hamzah and Hashim, Yumi Zuhanis Has-Yun (2014) Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design. Advances in Environmental Biology, 8 (3). pp. 734-743. ISSN 1998-1066 (O), 1995-0756 (P) http://www.aensiweb.com/old/aeb/2014/734-743.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English English
topic	T Technology (General) TP248.13 Biotechnology
spellingShingle	T Technology (General) TP248.13 Biotechnology Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
description	Xanthine oxidase (XO) is a key enzyme in hyperuricemia, catalyzing the oxidation of hypoxanthine to xanthine and then to uric acid. Excess serum accumulated with uric acid leads to a type of arthritis known as gout. In this study, development of process conditions for XO inhibitory activity from the leaves of Morinda elliptica was performed by using 70% methanol. Optimization of process parameters such as extraction temperature (ºC), extraction time (h), agitation speed (rpm) and ratio of sample to solvent (1g/ml) at five levels was carried out using central composite design (CCD) for the improvement of activity to treat gout. The analysis of variance demonstrated that the model F-value of 18.31 showed the significance of the model with R2 of 97.71%. The analysis revealed that the percentage of XO inhibitory activity was improved at 32 ºC, 30 h, 125 rpm and 1 g/15 ml of solvent. The optimized conditions were verified and the percentage of XO inhibitory activity obtained was 88.93%. The results are encouraging to formulate food, nutraceutical or pharmaceutical products incorporating natural xanthine oxidase inhibitor (XOI), an alternative to irresponsive synthetic XOI.
format	Article
author	Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun
author_facet	Jamal, Parveen Azmi, Saiful Mohammad Nizam Amid, Azura Mohd. Salleh, Hamzah Hashim, Yumi Zuhanis Has-Yun
author_sort	Jamal, Parveen
title	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_short	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_full	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_fullStr	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_full_unstemmed	Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
title_sort	development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design
publisher	AENSI Publications
publishDate	2014
url	http://irep.iium.edu.my/36413/ http://irep.iium.edu.my/36413/ http://irep.iium.edu.my/36413/1/734-743_Parveen_.pdf http://irep.iium.edu.my/36413/4/36413_Development%20and%20improvement%20of%20anti-gout_SCOPUS.pdf
first_indexed	2023-09-18T20:52:10Z
last_indexed	2023-09-18T20:52:10Z
_version_	1777410066678284288

Development and improvement of anti-gout property from aqueous-methanol extract of morinda elliptica using central composite design

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