Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002

Microalgae lipid is a promising feedstock for biodiesel production. The objective of this work was to screen growth factors affecting mutant Synechococcus sp. (PCC 7002) for high lipid production. The effect of 4 factors; nitrogen-phosporus- potassium (NPK) concentration, light intensity, temperatur...

Full description

Bibliographic Details
Main Authors: Shari, Ummi S. Halmi, Zainal, Mus'ab, Azmi, Azlin Suhaida, Amid, Azura, Jimat, Dzun Noraini, Ahmad Raus, Raha, Muyibi, Suleyman Aremu, Mustafa, Iswaibah, Salleh, Noraini
Format: Conference or Workshop Item
Language:English
English
Published: 2013
Subjects:
Online Access:http://irep.iium.edu.my/35078/
http://irep.iium.edu.my/35078/1/Ummi-screening_process_parameters-lipid.pdf
http://irep.iium.edu.my/35078/2/CIABiotech13_Tentative_Program.pdf
id iium-35078
recordtype eprints
spelling iium-350782014-02-12T01:58:42Z http://irep.iium.edu.my/35078/ Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002 Shari, Ummi S. Halmi Zainal, Mus'ab Azmi, Azlin Suhaida Amid, Azura Jimat, Dzun Noraini Ahmad Raus, Raha Muyibi, Suleyman Aremu Mustafa, Iswaibah Salleh, Noraini TP248.13 Biotechnology Microalgae lipid is a promising feedstock for biodiesel production. The objective of this work was to screen growth factors affecting mutant Synechococcus sp. (PCC 7002) for high lipid production. The effect of 4 factors; nitrogen-phosporus- potassium (NPK) concentration, light intensity, temperature and NaNO3 concentration on mutant strain growth and lipid production were studied. Design Expert Version 8.0 was used to analyze data. The experimental design selected was Min-Run Res IV which consists of 12 runs and the response surfaces measured were specific growth rate and yield of lipid. The extraction of lipid was conducted by chloroform/methanol solvents system. Based on the study, mutant Synechococcus sp. (PCC 7002) gave the highest specific growth rate; 0.3811 h-1 at 0% NPK, 2500 lux, 40oC and 1% NaNO3. Besides, the highest yield of lipid was obtained at condition of 0% NPK, 3500 lux, 30oC and 1% NaNO3. It implies that, NPK did not influence both growth and lipid production of mutant strain. On the other hand, NaNO3 plays important roles in both growth and lipid production. 2013-12-07 Conference or Workshop Item PeerReviewed application/pdf en http://irep.iium.edu.my/35078/1/Ummi-screening_process_parameters-lipid.pdf application/pdf en http://irep.iium.edu.my/35078/2/CIABiotech13_Tentative_Program.pdf Shari, Ummi S. Halmi and Zainal, Mus'ab and Azmi, Azlin Suhaida and Amid, Azura and Jimat, Dzun Noraini and Ahmad Raus, Raha and Muyibi, Suleyman Aremu and Mustafa, Iswaibah and Salleh, Noraini (2013) Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002. In: National Conference on Industry-Academia Joint Initiatives in Biotechnology 2013, 5 - 7 December 2013, Equatorial Hotel, Cameron Highlands.
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Shari, Ummi S. Halmi
Zainal, Mus'ab
Azmi, Azlin Suhaida
Amid, Azura
Jimat, Dzun Noraini
Ahmad Raus, Raha
Muyibi, Suleyman Aremu
Mustafa, Iswaibah
Salleh, Noraini
Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002
description Microalgae lipid is a promising feedstock for biodiesel production. The objective of this work was to screen growth factors affecting mutant Synechococcus sp. (PCC 7002) for high lipid production. The effect of 4 factors; nitrogen-phosporus- potassium (NPK) concentration, light intensity, temperature and NaNO3 concentration on mutant strain growth and lipid production were studied. Design Expert Version 8.0 was used to analyze data. The experimental design selected was Min-Run Res IV which consists of 12 runs and the response surfaces measured were specific growth rate and yield of lipid. The extraction of lipid was conducted by chloroform/methanol solvents system. Based on the study, mutant Synechococcus sp. (PCC 7002) gave the highest specific growth rate; 0.3811 h-1 at 0% NPK, 2500 lux, 40oC and 1% NaNO3. Besides, the highest yield of lipid was obtained at condition of 0% NPK, 3500 lux, 30oC and 1% NaNO3. It implies that, NPK did not influence both growth and lipid production of mutant strain. On the other hand, NaNO3 plays important roles in both growth and lipid production.
format Conference or Workshop Item
author Shari, Ummi S. Halmi
Zainal, Mus'ab
Azmi, Azlin Suhaida
Amid, Azura
Jimat, Dzun Noraini
Ahmad Raus, Raha
Muyibi, Suleyman Aremu
Mustafa, Iswaibah
Salleh, Noraini
author_facet Shari, Ummi S. Halmi
Zainal, Mus'ab
Azmi, Azlin Suhaida
Amid, Azura
Jimat, Dzun Noraini
Ahmad Raus, Raha
Muyibi, Suleyman Aremu
Mustafa, Iswaibah
Salleh, Noraini
author_sort Shari, Ummi S. Halmi
title Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002
title_short Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002
title_full Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002
title_fullStr Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002
title_full_unstemmed Screening of the process parameters for lipid production using mutant Synechococcus sp. PCC 7002
title_sort screening of the process parameters for lipid production using mutant synechococcus sp. pcc 7002
publishDate 2013
url http://irep.iium.edu.my/35078/
http://irep.iium.edu.my/35078/1/Ummi-screening_process_parameters-lipid.pdf
http://irep.iium.edu.my/35078/2/CIABiotech13_Tentative_Program.pdf
first_indexed 2023-09-18T20:50:23Z
last_indexed 2023-09-18T20:50:23Z
_version_ 1777409955090923520