Purification, characterization and kinetic properties of alpha-naphthyl acetate esterase from wheat flour (Triticum aestivum)
Plant-esterase from wheat flour was purified via aqueous two-phase system (ATPS). The enzyme crude extract was first filtered through a polyethersulfone OmegaTM ultrafiltration membrane, followed by two-step ATPS with PEG1000, NaH2PO4 and (NH4)2SO4. Based on previous studies, the first ATPS cons...
Main Authors: | , |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2018
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Subjects: | |
Online Access: | http://irep.iium.edu.my/32983/ http://irep.iium.edu.my/32983/ http://irep.iium.edu.my/32983/1/32983_Purification%2C%20characterization%20and%20kinetic.pdf |