Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet

Introduction: The collagen can be basically extracted by chemical and enzymatic hydrolysis. In industry, the chemical hydrolysis method is more commonly used. However, the enzymatic hydrolysis is more effective when the high nutritional value product will be produces based on the extracted materi...

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Main Authors:	Mokhtar, Nur Dhiya, Abdul Wahab, Widya, Ab. Hadi, Hazrina, Abu Hassan, Muhamad Shafiq, Haron, Normah, Muhamad Bunnori, Noraslinda
Format:	Article
Language:	English
Published:	IIUM Press 2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/32641/ http://irep.iium.edu.my/32641/ http://irep.iium.edu.my/32641/1/32641.pdf

id	iium-32641
recordtype	eprints
spelling	iium-326412017-01-16T06:33:59Z http://irep.iium.edu.my/32641/ Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet Mokhtar, Nur Dhiya Abdul Wahab, Widya Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Haron, Normah Muhamad Bunnori, Noraslinda RS Pharmacy and materia medica Introduction: The collagen can be basically extracted by chemical and enzymatic hydrolysis. In industry, the chemical hydrolysis method is more commonly used. However, the enzymatic hydrolysis is more effective when the high nutritional value product will be produces based on the extracted material. Chicken (Gallus gallus domesticus) feet are used as a source of bird collagen. Methods: Optimization of collagen extraction was investigated by using three different methods which were soaked in 5% lactic acid for 36 hours, 0.5 mol/L acetic acid with 0.1% papain and 0.5 mol/L acetic acid with 0.1% bromelain. Various properties such as percentage of end yield, concentration of protein, pH, swelling percentage and SDS-PAGE patterns of collagen from chicken feet were evaluated in order to determine the best method of extraction. Results: Type I collagen was considered as a major component of chicken feet collagen, which indicated by β chain and two distinct α-chains which are α1 and α2 that observed from the electrophoresis pattern of collagen extracted from all three methods. The lactic acid method showed the highest percentage of end yield and concentration of protein compared to the other two methods. However, lactic acid method showed the lowest swelling percentage and higher pH value. Conclusions: According to these results, soaked in 5% lactic acid for 36 hours is possible as the best collagen extraction method from chicken feet. IIUM Press 2016-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32641/1/32641.pdf Mokhtar, Nur Dhiya and Abdul Wahab, Widya and Ab. Hadi, Hazrina and Abu Hassan, Muhamad Shafiq and Haron, Normah and Muhamad Bunnori, Noraslinda (2016) Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet. International Medical Journal Malaysia (IMJM), 15 (Supplement issue). p. 37. E-ISSN 1823-4631 http://iiumedic.net/imjm/v1/download/Volume%2015%20Supplement/MRS2016-ABSTRACT-BOOK-37.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Mokhtar, Nur Dhiya Abdul Wahab, Widya Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Haron, Normah Muhamad Bunnori, Noraslinda Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
description	Introduction: The collagen can be basically extracted by chemical and enzymatic hydrolysis. In industry, the chemical hydrolysis method is more commonly used. However, the enzymatic hydrolysis is more effective when the high nutritional value product will be produces based on the extracted material. Chicken (Gallus gallus domesticus) feet are used as a source of bird collagen. Methods: Optimization of collagen extraction was investigated by using three different methods which were soaked in 5% lactic acid for 36 hours, 0.5 mol/L acetic acid with 0.1% papain and 0.5 mol/L acetic acid with 0.1% bromelain. Various properties such as percentage of end yield, concentration of protein, pH, swelling percentage and SDS-PAGE patterns of collagen from chicken feet were evaluated in order to determine the best method of extraction. Results: Type I collagen was considered as a major component of chicken feet collagen, which indicated by β chain and two distinct α-chains which are α1 and α2 that observed from the electrophoresis pattern of collagen extracted from all three methods. The lactic acid method showed the highest percentage of end yield and concentration of protein compared to the other two methods. However, lactic acid method showed the lowest swelling percentage and higher pH value. Conclusions: According to these results, soaked in 5% lactic acid for 36 hours is possible as the best collagen extraction method from chicken feet.
format	Article
author	Mokhtar, Nur Dhiya Abdul Wahab, Widya Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Haron, Normah Muhamad Bunnori, Noraslinda
author_facet	Mokhtar, Nur Dhiya Abdul Wahab, Widya Ab. Hadi, Hazrina Abu Hassan, Muhamad Shafiq Haron, Normah Muhamad Bunnori, Noraslinda
author_sort	Mokhtar, Nur Dhiya
title	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_short	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_full	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_fullStr	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_full_unstemmed	Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet
title_sort	extraction optimization and properties of collagen from chicken (gallus gallus domesticus) feet
publisher	IIUM Press
publishDate	2016
url	http://irep.iium.edu.my/32641/ http://irep.iium.edu.my/32641/ http://irep.iium.edu.my/32641/1/32641.pdf
first_indexed	2023-09-18T20:47:07Z
last_indexed	2023-09-18T20:47:07Z
_version_	1777409749160034304

Extraction optimization and properties of collagen from chicken (Gallus gallus domesticus) feet

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