Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk

E. coli O157:H7 is associated with life threatening diseases such as hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Raw milk is considered a high risk food as it is highly nutritious and serves as an ideal medium for bacterial growth. The aim...

Full description

Bibliographic Details
Main Authors:	Lye, Y. L., L., Afsah-Hejri, Chang, W.S., Loo, YY, Puspanadan, S, Kuan, CH, Goh, SG, Shahril, N, ., Rukayadi, Khatib, Alfi, John, YHT, ., Nishibuchi, Nakaguchi, Y, Son, R
Format:	Article
Language:	English
Published:	Faculty of Food Science and Technology, Universiti Putra Malaysia 2013
Subjects:	S Agriculture (General)
Online Access:	http://irep.iium.edu.my/32203/ http://irep.iium.edu.my/32203/ http://irep.iium.edu.my/32203/1/Ying_ling_et_al._IFRJ_2013.pdf

id	iium-32203
recordtype	eprints
spelling	iium-322032013-10-04T06:05:04Z http://irep.iium.edu.my/32203/ Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk Lye, Y. L. L., Afsah-Hejri Chang, W.S. Loo, YY Puspanadan, S Kuan, CH Goh, SG Shahril, N ., Rukayadi Khatib, Alfi John, YHT ., Nishibuchi Nakaguchi, Y Son, R S Agriculture (General) E. coli O157:H7 is associated with life threatening diseases such as hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Raw milk is considered a high risk food as it is highly nutritious and serves as an ideal medium for bacterial growth. The aim of this study was to investigate the prevalence of E. coli O157:H7 in raw cow, goat and buffalo milk samples. MPN-PCR method targeting the major virulence rfbE gene and fliCH7 gene of E. coli O157:H7 was used. Total of 177 raw milk samples were collected from local dairy farms in the state of Selangor, Malaysia. The highest prevalence of E. coli O157:H7 was found in raw cow milk (8.75%) followed by raw goat milk (7.32%) and raw buffalo milk (1.79%). The estimated quantity of E. coli O157:H7 in raw cow, goat and buffalo milk ranged from <30 MPN/g to 120 MPN/g. In raw cow and goat milk samples examined contain E. coli O157:H7 microbial load ranged from 30 to 120 MPN/g and 30 to 36 MPN/g, respectively. E. coli O157:H7 microbial load in buffalo milk samples was found to be the lowest, only 30 MPN/g. Results of this research provide useful information on biosafety of E. coli O157:H7 in raw milk marketed in Malaysia. Faculty of Food Science and Technology, Universiti Putra Malaysia 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32203/1/Ying_ling_et_al._IFRJ_2013.pdf Lye, Y. L. and L., Afsah-Hejri and Chang, W.S. and Loo, YY and Puspanadan, S and Kuan, CH and Goh, SG and Shahril, N and ., Rukayadi and Khatib, Alfi and John, YHT and ., Nishibuchi and Nakaguchi, Y and Son, R (2013) Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk. International Food Research Journal, 20 (2). pp. 1001-1005. ISSN 19854668 (P), 22317546 (O) http://www.ifrj.upm.edu.my/20%20%2802%29%202013/69%20IFRJ%2020%20%2802%29%202013%20Ying%20ling%20%28481%29.pdf
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	S Agriculture (General)
spellingShingle	S Agriculture (General) Lye, Y. L. L., Afsah-Hejri Chang, W.S. Loo, YY Puspanadan, S Kuan, CH Goh, SG Shahril, N ., Rukayadi Khatib, Alfi John, YHT ., Nishibuchi Nakaguchi, Y Son, R Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk
description	E. coli O157:H7 is associated with life threatening diseases such as hemorrhagic colitis (HC), hemolytic uremic syndrome (HUS) and thrombotic thrombocytopenic purpura (TTP). Raw milk is considered a high risk food as it is highly nutritious and serves as an ideal medium for bacterial growth. The aim of this study was to investigate the prevalence of E. coli O157:H7 in raw cow, goat and buffalo milk samples. MPN-PCR method targeting the major virulence rfbE gene and fliCH7 gene of E. coli O157:H7 was used. Total of 177 raw milk samples were collected from local dairy farms in the state of Selangor, Malaysia. The highest prevalence of E. coli O157:H7 was found in raw cow milk (8.75%) followed by raw goat milk (7.32%) and raw buffalo milk (1.79%). The estimated quantity of E. coli O157:H7 in raw cow, goat and buffalo milk ranged from <30 MPN/g to 120 MPN/g. In raw cow and goat milk samples examined contain E. coli O157:H7 microbial load ranged from 30 to 120 MPN/g and 30 to 36 MPN/g, respectively. E. coli O157:H7 microbial load in buffalo milk samples was found to be the lowest, only 30 MPN/g. Results of this research provide useful information on biosafety of E. coli O157:H7 in raw milk marketed in Malaysia.
format	Article
author	Lye, Y. L. L., Afsah-Hejri Chang, W.S. Loo, YY Puspanadan, S Kuan, CH Goh, SG Shahril, N ., Rukayadi Khatib, Alfi John, YHT ., Nishibuchi Nakaguchi, Y Son, R
author_facet	Lye, Y. L. L., Afsah-Hejri Chang, W.S. Loo, YY Puspanadan, S Kuan, CH Goh, SG Shahril, N ., Rukayadi Khatib, Alfi John, YHT ., Nishibuchi Nakaguchi, Y Son, R
author_sort	Lye, Y. L.
title	Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk
title_short	Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk
title_full	Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk
title_fullStr	Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk
title_full_unstemmed	Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk
title_sort	risk of escherichia coli o157:h7 transmission linked to the consumption of raw milk
publisher	Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate	2013
url	http://irep.iium.edu.my/32203/ http://irep.iium.edu.my/32203/ http://irep.iium.edu.my/32203/1/Ying_ling_et_al._IFRJ_2013.pdf
first_indexed	2023-09-18T20:46:29Z
last_indexed	2023-09-18T20:46:29Z
_version_	1777409708847529984

Risk of Escherichia coli O157:H7 transmission linked to the consumption of raw milk

Similar Items