Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system

Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system

The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol f...

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Main Authors: Rahmani, Anoshep, Selamat, Jinap, Khatib, Alfi, Chin Ping, Tan
Format: Article
Language:English
Published: Taylor & Francis Group 2013
Subjects:
QD Chemistry
Online Access:http://irep.iium.edu.my/32047/
http://irep.iium.edu.my/32047/
http://irep.iium.edu.my/32047/
http://irep.iium.edu.my/32047/1/rahmani_JLCRT_2013.pdf
id iium-32047
recordtype eprints
spelling iium-320472013-09-30T02:53:13Z http://irep.iium.edu.my/32047/ Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system Rahmani, Anoshep Selamat, Jinap Khatib, Alfi Chin Ping, Tan QD Chemistry The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol followed by a multifunctional immunoaffinity AOZ column clean-up was applied for the extraction of these mycotoxins before HPLC analysis. A photochemical reactor enhance derivatization system (PHRED) was used between the HPLC column and fluorescence detector, in order to enhance the fluorescence intensity of aflatoxin B1 and G1. The best chromatographic separation for the simultaneous determination of the nominated mycotoxins was achieved under optimal HPLC conditions. Recoveries were 77.31–104.1% for cereal samples spiked at 6.5,10, and 100 ng=g for total aflatoxins (AFs), OTA, and ZEA, respectively; whereas, the precisions were 2.45–11.14%. Limits of detection (LOD) for AFs, OTA, and ZEA were 0.004–0.012,0.05, and 0.5 ng=g, respectively; whereas. limits of quantification (LOQ) were 0.015–0.05, 0.2, and 2ng=g, correspondingly. Taylor & Francis Group 2013 Article PeerReviewed application/pdf en http://irep.iium.edu.my/32047/1/rahmani_JLCRT_2013.pdf Rahmani, Anoshep and Selamat, Jinap and Khatib, Alfi and Chin Ping, Tan (2013) Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system. Journal of Liquid Chromatography & Related Technologies, 36 (5). pp. 600-617. ISSN 1082-6076 print/1520-572X online http://www.tandfonline.com/doi/full/10.1080/10826076.2012.670182#.UkjtQn_RI9k 0.1080/10826076.2012.670182
repository_type Digital Repository
institution_category Local University
institution International Islamic University Malaysia
building IIUM Repository
collection Online Access
language English
topic QD Chemistry
spellingShingle QD Chemistry
Rahmani, Anoshep
Selamat, Jinap
Khatib, Alfi
Chin Ping, Tan
Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system
description The objective of the study was to develop and validate an HPLC-FLD method, after sample preparation and derivatization, for the simultaneous determination of aflatoxins (B1, B2, G1, and G2), ochratoxin A (OTA), and zearalenone (ZEA) in cereals. A solid–liquid extraction approach using 80% methanol followed by a multifunctional immunoaffinity AOZ column clean-up was applied for the extraction of these mycotoxins before HPLC analysis. A photochemical reactor enhance derivatization system (PHRED) was used between the HPLC column and fluorescence detector, in order to enhance the fluorescence intensity of aflatoxin B1 and G1. The best chromatographic separation for the simultaneous determination of the nominated mycotoxins was achieved under optimal HPLC conditions. Recoveries were 77.31–104.1% for cereal samples spiked at 6.5,10, and 100 ng=g for total aflatoxins (AFs), OTA, and ZEA, respectively; whereas, the precisions were 2.45–11.14%. Limits of detection (LOD) for AFs, OTA, and ZEA were 0.004–0.012,0.05, and 0.5 ng=g, respectively; whereas. limits of quantification (LOQ) were 0.015–0.05, 0.2, and 2ng=g, correspondingly.
format Article
author Rahmani, Anoshep
Selamat, Jinap
Khatib, Alfi
Chin Ping, Tan
author_facet Rahmani, Anoshep
Selamat, Jinap
Khatib, Alfi
Chin Ping, Tan
author_sort Rahmani, Anoshep
title Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system
title_short Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system
title_full Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system
title_fullStr Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system
title_full_unstemmed Simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated HPLC-FLD method and PHRED derivatization system
title_sort simultaneous determination of aflatoxins, ochratoxin a, and zearalenone in cereals using a validated hplc-fld method and phred derivatization system
publisher Taylor & Francis Group
publishDate 2013
url http://irep.iium.edu.my/32047/
http://irep.iium.edu.my/32047/
http://irep.iium.edu.my/32047/
http://irep.iium.edu.my/32047/1/rahmani_JLCRT_2013.pdf
first_indexed 2023-09-18T20:46:14Z
last_indexed 2023-09-18T20:46:14Z
_version_ 1777409693650518016

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