Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres

A simple, rapid, precise and highly accurate RP-HPLC method was developed and validated for determination of alpha-mangostin content extracted from PLGA-microspheres. Method was developed using a silica-based deactivated C-18 column (4.6×100 mm, 3 μm) with a mobile phase of 70-80 % v/v acetonitril...

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Main Authors:	Elsaid Ali, Aimen Abdo, Muhammad, Taher, Helaluddin , Abul Bashar Mohammed, Mohamed, Farahidah
Format:	Article
Language:	English
Published:	OMICS Publishing Group 2012
Subjects:	RS Pharmacy and materia medica
Online Access:	http://irep.iium.edu.my/30074/ http://irep.iium.edu.my/30074/ http://irep.iium.edu.my/30074/ http://irep.iium.edu.my/30074/1/Development_and_validation_of_analyticcal_method_by_RP-HPLC_for_quantification.pdf

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spelling	iium-300742013-10-17T04:00:39Z http://irep.iium.edu.my/30074/ Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres Elsaid Ali, Aimen Abdo Muhammad, Taher Helaluddin , Abul Bashar Mohammed Mohamed, Farahidah RS Pharmacy and materia medica A simple, rapid, precise and highly accurate RP-HPLC method was developed and validated for determination of alpha-mangostin content extracted from PLGA-microspheres. Method was developed using a silica-based deactivated C-18 column (4.6×100 mm, 3 μm) with a mobile phase of 70-80 % v/v acetonitrile (A) and 0.1% v/v orthophosphoric acid (B), with the following pre-determined timed-gradient program: 70% (A) isocratic for 6 min, 70-75% (A) in 1.2 min, 75-80% (A) in 0.4 min, 80% (A) isocratic for 2.4 min, 80-70% (A) in 0.4 min, finally 70% (A)isocratic for 5 min, with a flow rate of 1 mL/min, detected at 320 nm by a UV detector. Linearity was obtained over the range of 1-200 μg/mL with r2=0.9995. The precision was achieved based on repeatability and intermediate precision with RSD of 0.13-0.6% and 0.57-1.2%, respectively. Percent recovery of 100.55% to 103.82% with RSD 0.086 - 0.15 implied high accuracy of the method. Limit of detection and limit of quantitation were 0.038 and 0.121 μg/ml, respectively suggesting good sensitivity of the method. The method is envisaged to be effectively used for routine quality control assay for encapsulated alpha-mangostin in PLGA microspheres. OMICS Publishing Group 2012 Article PeerReviewed application/pdf en http://irep.iium.edu.my/30074/1/Development_and_validation_of_analyticcal_method_by_RP-HPLC_for_quantification.pdf Elsaid Ali, Aimen Abdo and Muhammad, Taher and Helaluddin , Abul Bashar Mohammed and Mohamed, Farahidah (2012) Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres. Journal of Analytical & Bioanalytical Techniques, 3 (7). pp. 1-5. ISSN 2155-9872 http://www.omicsonline.org/2155-9872/2155-9872-3-155.digital/2155-9872-3-155.html 10.4172/2155-9872.1000155
repository_type	Digital Repository
institution_category	Local University
institution	International Islamic University Malaysia
building	IIUM Repository
collection	Online Access
language	English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Elsaid Ali, Aimen Abdo Muhammad, Taher Helaluddin , Abul Bashar Mohammed Mohamed, Farahidah Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres
description	A simple, rapid, precise and highly accurate RP-HPLC method was developed and validated for determination of alpha-mangostin content extracted from PLGA-microspheres. Method was developed using a silica-based deactivated C-18 column (4.6×100 mm, 3 μm) with a mobile phase of 70-80 % v/v acetonitrile (A) and 0.1% v/v orthophosphoric acid (B), with the following pre-determined timed-gradient program: 70% (A) isocratic for 6 min, 70-75% (A) in 1.2 min, 75-80% (A) in 0.4 min, 80% (A) isocratic for 2.4 min, 80-70% (A) in 0.4 min, finally 70% (A)isocratic for 5 min, with a flow rate of 1 mL/min, detected at 320 nm by a UV detector. Linearity was obtained over the range of 1-200 μg/mL with r2=0.9995. The precision was achieved based on repeatability and intermediate precision with RSD of 0.13-0.6% and 0.57-1.2%, respectively. Percent recovery of 100.55% to 103.82% with RSD 0.086 - 0.15 implied high accuracy of the method. Limit of detection and limit of quantitation were 0.038 and 0.121 μg/ml, respectively suggesting good sensitivity of the method. The method is envisaged to be effectively used for routine quality control assay for encapsulated alpha-mangostin in PLGA microspheres.
format	Article
author	Elsaid Ali, Aimen Abdo Muhammad, Taher Helaluddin , Abul Bashar Mohammed Mohamed, Farahidah
author_facet	Elsaid Ali, Aimen Abdo Muhammad, Taher Helaluddin , Abul Bashar Mohammed Mohamed, Farahidah
author_sort	Elsaid Ali, Aimen Abdo
title	Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres
title_short	Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres
title_full	Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres
title_fullStr	Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres
title_full_unstemmed	Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres
title_sort	development and validation of analytical method by rp-hplc for quantification of alpha-mangostin encapsulated in plga microspheres
publisher	OMICS Publishing Group
publishDate	2012
url	http://irep.iium.edu.my/30074/ http://irep.iium.edu.my/30074/ http://irep.iium.edu.my/30074/ http://irep.iium.edu.my/30074/1/Development_and_validation_of_analyticcal_method_by_RP-HPLC_for_quantification.pdf
first_indexed	2023-09-18T20:44:09Z
last_indexed	2023-09-18T20:44:09Z
_version_	1777409562595295232

Development and validation of analytical method by RP-HPLC for quantification of Alpha-Mangostin Encapsulated in PLGA microspheres

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