Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology
Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli, BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surf...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Springer
2012
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/ http://irep.iium.edu.my/23637/1/Bala_final_2191-0855-2-12.pdf |